Evaluation method for rough skin

An evaluation method, skin technology, applied in the direction of material inspection products, instruments, disease diagnosis, etc., can solve the problem of unconfirmed expression of fibroblasts, etc., and achieve the effect of less burden

Active Publication Date: 2014-05-21
FUAN KERU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

On the other hand, expression in fibroblasts was not confirmed

Method used

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  • Evaluation method for rough skin
  • Evaluation method for rough skin
  • Evaluation method for rough skin

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Experimental program
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Embodiment

[0061] 1. Determination of the expression level of galectin-7 in various parts of the human body

[0062]

[0063] Samples were collected from the cheeks, inner forearms, and buttocks of 35 healthy, normal-skinned men and women, including those with atopic dermatitis (AD) in the past.

[0064]

[0065] A stratum corneum test piece (ASAHIBIOMED) was pasted on the cheeks, arms, and buttocks of the test subject, and the stratum corneum was collected. Use the glass bead method (a method in which the analyte, glass beads with a diameter of about 2 mm, and the extraction solution are placed in a container to extract by shaking) from the cuticle test piece using 500 μl of T-PER extraction buffer (Tissue Protein Extraction Reagent (Tissue Protein Extraction) Reagent) manufactured by Thermo Fisher Scientific Co., Ltd. (Thermo Scientific) (product #78510)) was used to extract the stratum corneum protein. The amount of protein in each sample was determined using Pierce BCA protein A...

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Abstract

The utility model aims to provide a novel evaluation method which uses a biochemical index that does not cause load to a user and can easily and truly evaluate whether the skin is the rough skin. A keratoderma is collected invasively through an adhesive tape stripping method. Degree of the rough skin is evaluated through measuring expression amount of galectin-7 contained therein.

Description

technical field [0001] The present invention relates to an evaluation method for rough skin. Background technique [0002] The skin of the human body is often in contact with the outside world and is subject to various stimuli. Its representative stimulus is ultraviolet light. Even if ultraviolet rays do not cause significant changes in the appearance of the skin, they can induce DNA cleavage in skin cells, denaturation of collagen, etc., and cause skin aging. UV rays constantly stress the skin. In addition, for women, daily activities such as washing the face and putting on makeup can also irritate the skin, and when such external stimuli accumulate on the skin, it will form a state that is considered rough skin. Rough skin refers to a temporary symptom, but the state where the symptom persists is called "rough skin". [0003] "Rough skin" is a general term for the persistent state of dry, parched skin symptoms, the most prominent clinical manifestation of which is scal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCA61B10/02G01N33/5091G01N33/6881G01N2800/40
Inventor 安田知永禹幸玉
Owner FUAN KERU
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