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Application of depolymerized holothurian glycosaminolycan in medicine preparation

A technology of glycosaminoglycan and sea cucumber, which is applied in the application field of depolymerized sea cucumber glycosaminoglycan in pharmaceuticals, and can solve problems such as strong use monitoring, potential safety hazards, and difficulty in optimal dosage

Inactive Publication Date: 2014-06-04
SHANGHAI KAIRUN BIOLOGY MEDICINE LIMITED LIABILITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while highly effective, warfarin carries a risk of serious and even fatal bleeding
In addition, due to the large individual differences in pharmacokinetics and the influence of diet, and the complex drug interactions, it is difficult to use optimal doses of warfarin in clinical practice. In addition, heparin and low molecular weight heparin drugs, Clinical use can easily lead to bleeding, and people with different constitutions need to be monitored closely
Therefore, the anticoagulant drugs currently used clinically all have certain side effects. In view of the aging population, the increase in the incidence of thrombotic diseases, and the widespread use of existing anticoagulant drugs in the clinical prevention and treatment of thrombotic diseases and the seriousness of its security risks

Method used

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  • Application of depolymerized holothurian glycosaminolycan in medicine preparation
  • Application of depolymerized holothurian glycosaminolycan in medicine preparation
  • Application of depolymerized holothurian glycosaminolycan in medicine preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Extraction of Sea Cucumber Glycosaminoglycans

[0040] Weigh 3 kg of Yuzu sea cucumber medicinal material and soak in water overnight. Drain the water from the body wall of sea cucumbers, mince them, weigh them and add water to 27Kg, place them in a water bath at 58°C, adjust the pH to 7.8±0.2, add 60ml of hydrolytic protease Alcalase (Novozymes (Shenyang) Biotechnology Co., Ltd. Company) stirring, enzymatic hydrolysis for 4 hours, inactivation above 85°C for 7 minutes, cooling to 49°C±2°C, adding sodium hydroxide to adjust the pH to 7.9±0.2, and adding 0.2-0.25% compound pancreatin (Wuxi City Xuemei Enzyme Preparation Technology Co., Ltd. (Xuemei Brand) stirred for 4.5 hours, boiled for 6 minutes, and cooled. Centrifuge at 4°C, collect the supernatant, add hydrochloric acid to adjust the pH to 2.6±0.2, refrigerate at 4°C for 2.5 hours, centrifuge, collect the supernatant, add sodium hydroxide to adjust the pH to 6.9±0.2, add 0.9 times (v / v) Ethanol, stand overnig...

Embodiment 2

[0051] The extraction of sea cucumber glycosaminoglycans is the same as in Example 1.

[0052] The preparation method of depolymerized sea cucumber glycosaminoglycan is as follows:

[0053] Dissolve the above sea cucumber glycosaminoglycan product with 5.1% acetic acid, add hydrogen peroxide with a weight concentration of 30%, so that the weight concentration of hydrogen peroxide in the solution is 5.1%, and depolymerize at 60°C for 25 hours;

[0054] Neutralize the depolymerization solution to neutral with sodium hydroxide, add ethanol with 4 times the volume of the depolymerization solution for alcohol precipitation, let stand, centrifuge, collect the precipitate, and obtain the crude product of depolymerized sea cucumber glycosaminoglycan;

[0055] Dry the crude product, dissolve it in water with 5 times the weight of the crude product, pass it through a sephadex-G100 column, elute with 0.05mol / l sodium chloride for 5 column volumes, collect the eluate with a molecular weig...

Embodiment 3

[0059] The extraction of sea cucumber glycosaminoglycans is the same as in Example 1.

[0060] The preparation method of depolymerized sea cucumber glycosaminoglycan is as follows:

[0061] Dissolve the above-mentioned sea cucumber glycosaminoglycan product with 4.9% acetic acid, add hydrogen peroxide with a weight concentration of 30%, so that the weight concentration of hydrogen peroxide in the solution is 5.3%, and depolymerize at 58°C for 20 minutes;

[0062] Neutralize the depolymerization solution to neutral with sodium hydroxide, add ethanol with 4 times the volume of the depolymerization solution for alcohol precipitation, let stand, centrifuge, collect the precipitate, and obtain the crude product of depolymerized sea cucumber glycosaminoglycan;

[0063] Dry the crude product, dissolve it in water with 5 times the weight of the crude product, pass through a sephadex-G100 column, elute with 0.05 mol / l sodium chloride for 5 column volumes, collect the eluate with a mole...

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Abstract

The invention discloses an application of depolymerized holothurian glycosaminolycan in medicine preparation. The weight-average molecular weight of the depolymerized holothurian glycosaminolycan is 51,000Da to 54,500Da. The test on animals proves that when a certain dosage (3-100mg / kg) of the depolymerized holothurian glycosaminolycan, the weight-average molecular weight of which is 51,000Da to 54,500Da, is subcutaneously injected, the depolymerized holothurian glycosaminolycan has obvious anti-freezing activity. The invention gives full play to the advantages of the traditional Chinese medicine by adopting the modern science and technology. Lots of experimental studies prove that the preparation can be used for safely and effectively preventing and treating thrombotic diseases including deep venous thrombosis, thrombophilia or venous thromboembolism of pregnant women, blood clot formation during hematodialysis and the like.

Description

technical field [0001] The invention relates to a medical application of depolymerized sea cucumber glycosaminoglycan, in particular to the application of depolymerized sea cucumber glycosaminoglycan with a weight average molecular weight of 51,000Da to 54,500Da in the preparation of medicines for preventing and treating thrombotic diseases, including the treatment of deep Venous thrombosis, and its application in the prevention of thrombosis during hemodialysis treatment and the prevention and treatment of thrombosis after surgery. Background technique [0002] Thrombotic diseases refer to diseases caused by thrombosis or thromboembolism. Thrombosis: The process by which the formed components of blood form an embolus locally in a blood vessel or in the inner lining of the heart. According to the composition, it can be divided into: platelet thrombus, red blood cell thrombus, fibrin thrombus, and mixed thrombus; according to the part of formation, it can be divided into: ve...

Claims

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Application Information

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IPC IPC(8): A61K31/726A61P7/02A61P9/10
Inventor 王志国刘全海董玉琼
Owner SHANGHAI KAIRUN BIOLOGY MEDICINE LIMITED LIABILITY
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