Microfluidic solution concentration generation chip

Abstract

The present invention discloses a microfluidic solution concentration generation chip, which comprises a culture layer arranged in a laminated manner, an elastic separation membrane layer and a driver layer, wherein the elastic separation membrane layer is positioned between the culture layer and the driver layer, a plurality of culture units are distributed on the culture layer in a parallel manner, first drive groove channels are distributed on the drive layer, the first drive groove channel and the culture unit form a cross on a first position, the culture unit is separated into the upper culture unit and the lower culture unit with the first position, and the volumes of the lower culture units of each culture unit are different. With the microfluidic solution concentration generation chip, a plurality of solution concentrations can be rapidly generated, the microorganism culture can be performed in the generated different solution concentrations, the occupation area is small, array is easily achieved, the accurate flow rate control or the long time balance is not required to be performed, sample injection is simple, the particular gradient concentration can be obtained according to requirements, and the diverse gradient concentration requirements can be met.

Description

technical field [0001] The invention belongs to the field of microfluidics, in particular to a microfluidic solution concentration generating chip. Background technique [0002] In the process of industrial microbial fermentation production, efficient microbial transformation is jointly determined by high-yield strains and optimal culture conditions. Only by optimizing the culture conditions of high-yield strains can the ultimate goal of high yield be achieved. It can be said that the optimization of culture conditions is one of the keys to industrial microorganism research. However, there are significant differences in the culture conditions of different microorganisms. Not only do they have different requirements for the types and contents of the main carbon sources, nitrogen sources, and phosphorus sources, but they also have diverse requirements for other trace components, such as growth factors such as vitamins, and metal elements. Therefore, the optimization of cultur...

AI Technical Summary

Problems solved by technology

However, the structure of each unit in the reaction array is the same, and the concentration of the solution produced by mixing the units is the same, which cannot be directly used to generate various solution concentrations in batches, and cannot be directly used for diversified concentration gradient experiments.

Images