A Strain of Eubacterium mucilage and Its Application

A technology of slime Eubacterium and Bacteroides monomorpha, which is applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of environmental pollution and high cost of chemical synthesis, and achieve the effects of no environmental pollution, simple process and low production cost

Inactive Publication Date: 2015-11-18
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main way to obtain enterodiol is chemical synthesis, but the cost of chemical synthesis is high and pollutes the environment

Method used

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  • A Strain of Eubacterium mucilage and Its Application
  • A Strain of Eubacterium mucilage and Its Application
  • A Strain of Eubacterium mucilage and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1. Isolation and Identification of Eubacterium limosum ZL-II

[0078] 1. Isolation of Eubacterium limosum ZL-II

[0079] Collect fresh human or mouse feces samples, enrich them with cooked meat medium to obtain intestinal flora samples, inoculate them into 5 mL of carbon-free medium, add 100 mg of flaxseed meal (particle size 200 μm~1000 μm) as the substrate, 37 ℃ anaerobic culture for 48h. Take 10 μL of carbon source-free medium bacterial solution, and make 10-fold serial dilutions. take 10 －6Spread the two-fold dilution on the plate and incubate anaerobically at 37°C for 24h. Pick a single colony and inoculate it into 5 mL of carbon-free medium, add 100 mg of flaxseed meal (particle size: 200 μm to 1000 μm) as a substrate, and perform HPLC detection after 2 days of cultivation to screen single colonies that can produce enterodiol. Take 10 μL of the active bacteria solution and add it to the cooked meat liquid medium to enrich the bacteria, 10 -5 The doubl...

Embodiment 2

[0095] Example 2. Application of Eubacterium limosum ZL-IICGMCCNo.6846

[0096] This example relates to the application of Eubacterium limosum ZL-IICGMCCNo.6846 in the production of enterodiol, during which it involves the application of Eubacterium limosum ZL-IICGMCCNo.6846 in the production of 4,4′-dihydroxyenterediol ( DHEND) applications. details as follows:

[0097] 1. Research on the production of enterodiol from linseed meal by a mixture of three strains of bacteria (1)

[0098] 1. Bacteria enrichment: Take Eubacteriumlimosum ZL-IICGMCCNo.6846 bacterial liquid (the cell content is 5×10 7 cfu / mL), Bacteroides uniformis Strain-ICGMCCNo.4897 bacterial liquid (cell content of 7×10 7 cfu / mL) and Eggerthellalenta (Eggerthellalenta) ZL-IIICGMCCNo.6847 bacterial liquid (cell content of 7×10 7 cfu / mL) each 0.5mL, mixed to obtain a mixed bacterial liquid. Take 0.5 mL of the mixed bacterial solution, inoculate it into 5 mL of anaerobic liquid medium, and incubate anaerobicall...

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Abstract

The invention discloses a Eubacterium limosum and its application. The Eubacterium limosum is Eubacterium limosum ZL-II concretely, and has a preservation number of CGMCC No.6846 in China General Microbiological Culture Collection Center, and the application of the Eubacterium limosum is the application of the Eubacterium limosum ZL-II with the preservation number of CGMCC No.6846 in enterodiol production concretely. The Eubacterium limosum ZL-II with the preservation number of CGMCC No.6846 plays a great role in the preparation of a phytoestrogen component enterodiol by biologically converting a natural product. An enterodiol production technology provided by the invention has the advantages of simplicity, low production cost, no environmental pollution, suitableness for large scale production, and important economic benefit.

Description

technical field [0001] The present invention relates to a mucilage eubacterium and its application, in particular to a mucilage eubacterium and its application in producing enterodiol. Background technique [0002] In recent years, phytoestrogens have attracted widespread attention due to their extensive physiological and pharmacological effects. Enterodiol (END) is an internationally recognized phytoestrogen, which has bidirectional regulatory effects of estrogen-like and anti-estrogen, as well as various pharmacological activities such as tumor inhibition, anti-oxidation, prevention and treatment of cardiovascular diseases. [0003] Enterodiol mainly exists in mammals and is also known as mammalian lignans. At present, the main way to obtain enterodiol is chemical synthesis, but the cost of chemical synthesis is high and pollutes the environment. The results of the study show that the human intestinal flora can convert a variety of lignans in plants into enterodiols, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/00C12P39/00C12P7/22C12R1/01
Inventor 杨东辉刘树林朱红云李淼鑫
Owner PEKING UNIV
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