Method for identifying soybean aphid physiological races

A technology for soybean aphids and physiological races, applied in animal husbandry and other fields, can solve the problems of lack of aphid-resistant gene soybean resources and heavy identification workload, and achieve reliable identification results, strong operability, and easy-to-obtain materials

Inactive Publication Date: 2014-06-25
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
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Problems solved by technology

The identification of soybean aphid biotypes is very important for the targeted selection of resistant soybean varieties and the cultivation of resistant soybean resources. However, there is no s

Method used

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  • Method for identifying soybean aphid physiological races
  • Method for identifying soybean aphid physiological races
  • Method for identifying soybean aphid physiological races

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Identification of Physiological Races of Shanghai Soybean Aphid and Jinan Soybean Aphid

[0026] 1. In the greenhouse, isolate two "independent spaces" that are far apart and separated by double-layer gauze (it can also be two independent small greenhouses). 7 soybean identification hosts (Donnong 47, Williams82, Dowling, PI200538, PI567543C, PI567301B, P203) were sown in 1:1:1 peat, vermiculite, and perlite), and 5 replicates were set for each identification host. After about 12 days, the soybean seedlings grow to the V1 growth stage, that is, the first three compound leaves are fully expanded;

[0027] 2. Artificial inoculation of aphids. The Shanghai and Jinan soybean aphids cultivated on the susceptible soybean variety "Dongnong 47" were inoculated with 5 aphids to the fronts of three compound leaves of 7 soybean plants with a wet brush, and the aphids were inoculated. Afterwards, water management according to conventional cultivation measures;

[0028] 3. 14 days...

Embodiment 2

[0044] Identification of Physiological Races of Changchun Soybean Aphid and Guiyang Soybean Aphid

[0045]1. In the greenhouse, isolate two "independent spaces" that are far apart and separated by double-layer gauze (it can also be two independent small greenhouses). 7 soybean identification hosts (Donnong 47, Williams82, Dowling, PI200538, PI567543C, PI567301B, P203) were sown in 1:1:1 peat, vermiculite, and perlite), and 5 replicates were set for each identification host. After about 12 days, the soybean seedlings grow to the V1 growth stage, that is, the first three compound leaves are fully expanded;

[0046] 2. Artificial inoculation of aphids. The Changchun and Guiyang soybean aphids cultivated on the susceptible soybean variety "Dongnong 47" were inoculated with 5 aphids to the fronts of three compound leaves of 7 soybean plants with a wet brush, and the aphids were inoculated. Afterwards, water management according to conventional cultivation measures;

[0047] 3. 14...

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Abstract

The invention provides a method for identifying soybean aphid physiological races. The method for combining identification of the number of bred aphids with a differential host under a greenhouse condition and representation of plant diseases is adopted, the soybean aphid physiological races from different sources can be identified fast, targeted soybean aphid race resisting resource screening, new variety breeding and other studies are carried out, bases are provided for determining the target of comprehensive preventing and treating of the soybean aphids and aphid resisting breeding, and the identification method is fast and high in operability, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of identification of plant diseases and insect pests, and in particular relates to identification of physiological races of soybean aphids and identification of aphid resistance, in particular to a method for identification of physiological races of soybean aphids. Background technique [0002] Soybean aphid [Aphis glycines Matsumura (Hemiptera: Aphididae)] is one of the main pests of soybean seedlings, belonging to Hemiptera Aphididae. Before 2000, soybean aphids mainly occurred in China, the Philippines, Thailand, North Korea, South Korea, Indonesia and Russia, and then successively invaded the United States, Canada and Australia. With the intensification of climate warming, soybean aphids are becoming more and more serious and have become a worldwide agricultural pest of widespread concern. The soybean aphid is an oligophagous, heterohost insect. The identified summer hosts in my country include soybean...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 钟云鹏王彪闫军辉姚陆铭武天龙
Owner SHANGHAI JIAO TONG UNIV
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