Method for preparing rice nuclear male-sterile line through hybridization

Abstract

The invention discloses a method for preparing a rice nuclear male-sterile line through hybridization. Perpetual nuclear male-sterile rice is obtained by hybridizing a photoperiod-temperature sensitive nuclear male-sterile line with a low-temperature sensitive nuclear male-sterile line with identical agronomic trait. Hybrid rice is prepared by taking the perpetual nuclear male-sterile rice as a female parent and the conventional rice as a male parent. The perpetual nuclear male-sterile line is applied to rice hybrid advantage utilization, so that the shortcoming that hybrid seed production failure caused by instable fertility of a sterile line obtained by a two-line method is overcome, and suppression caused by cytoplasm of a hybrid rice sterile line obtained by a three-line method can be avoided; the rice utilization rate is improved favorably, and the high-quality and high-yield hybrid rice can be easily cultivated; safe and sustainable development of the hybrid rice is propelled.

Description

technical field [0001] The invention relates to a method for preparing a rice nuclear sterile line by hybridization. Background technique [0002] The technical core of three-line hybrid rice is hybridization to prepare cytoplasmic sterile lines. In 1970, Yuan Longping, Li Bihu and others discovered a cytoplasmic male sterile plant in Hainan wild rice. In 1973, the technical system of hybrid seed breeding of sterile line and maintainer line and hybrid seed breeding of sterile line and restorer line was successfully researched, that is, three-line matching. With the large-scale promotion of three-line hybrid rice, it has made great contributions to my country's food security. However, because the cytoplasmic male sterility used by the three-line method is restricted by the restoration relationship, it is difficult to breed new CMS lines and new restorer lines, and the utilization rate of rice resources is lower than 10%. With the successful research of photothermo-sensitiv...

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Problems solved by technology

[0003] The invention purpose of the present invention has two: the one, provide a kind of method of hybridization preparation rice nuclear male sterile line, aim to solve the fertility instability of the male sterile line in the existing two-line hybrid rice, that is, when hybrid seed production Due to the influence of environmental factors, the sterile line restores the fertility and leads to the failure of hybrid seed production; the second is to avoid the restriction of the cytoplasm of the three-line method, so as to improve the utilization rate of rice resources and promote the selection of more high-yield and high-quality hybrid rice varieties

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