Method for preparing rice nuclear male-sterile line through hybridization

A technology of nuclear sterile line and hybrid rice, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of unstable fertility of sterile lines, etc., and achieve the goal of overcoming unstable fertility and unfreedom , the effect of expanding the utilization rate

Inactive Publication Date: 2014-07-02
HUNAN NORMAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0003] The invention purpose of the present invention has two: the one, provide a kind of method of hybridization preparation rice nuclear male sterile line, aim to solve the fertility instability of the male sterile line in the existing two-line hybrid rice, that is, when hybrid seed production Due to the influence of environ

Method used

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  • Method for preparing rice nuclear male-sterile line through hybridization
  • Method for preparing rice nuclear male-sterile line through hybridization

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Embodiment Construction

[0010] See figure 1 with figure 2 , the method for hybridization of the present invention to prepare the rice GMS line is as follows:

[0011] First use backcross technology to prepare rice photothermosensitive genic male sterile lines and low temperature sensitive genic male sterile lines with completely consistent agronomic traits, and then use photothermosensitive genic male sterile lines and low temperature sensitive genic male sterile lines to produce lifelong genic male sterile lines Rice, orthogonal or reverse crossing is not restricted, and can be determined according to needs. Hybrid rice is obtained by crossing the life-long male sterile rice and conventional rice.

[0012] The rice lifelong male sterile line (female parent) provided by the invention can be hybridized with any conventional rice (male parent), and the hybrid F1 generation is normally fertile.

[0013] The following are tests and results of the present invention:

[0014] Experiment 1: The ric...

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Abstract

The invention discloses a method for preparing a rice nuclear male-sterile line through hybridization. Perpetual nuclear male-sterile rice is obtained by hybridizing a photoperiod-temperature sensitive nuclear male-sterile line with a low-temperature sensitive nuclear male-sterile line with identical agronomic trait. Hybrid rice is prepared by taking the perpetual nuclear male-sterile rice as a female parent and the conventional rice as a male parent. The perpetual nuclear male-sterile line is applied to rice hybrid advantage utilization, so that the shortcoming that hybrid seed production failure caused by instable fertility of a sterile line obtained by a two-line method is overcome, and suppression caused by cytoplasm of a hybrid rice sterile line obtained by a three-line method can be avoided; the rice utilization rate is improved favorably, and the high-quality and high-yield hybrid rice can be easily cultivated; safe and sustainable development of the hybrid rice is propelled.

Description

technical field [0001] The invention relates to a method for preparing a rice nuclear sterile line by hybridization. Background technique [0002] The technical core of three-line hybrid rice is hybridization to prepare cytoplasmic sterile lines. In 1970, Yuan Longping, Li Bihu and others discovered a cytoplasmic male sterile plant in Hainan wild rice. In 1973, the technical system of hybrid seed breeding of sterile line and maintainer line and hybrid seed breeding of sterile line and restorer line was successfully researched, that is, three-line matching. With the large-scale promotion of three-line hybrid rice, it has made great contributions to my country's food security. However, because the cytoplasmic male sterility used by the three-line method is restricted by the restoration relationship, it is difficult to breed new CMS lines and new restorer lines, and the utilization rate of rice resources is lower than 10%. With the successful research of photothermo-sensitiv...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 陈良碧梁满中田炜戴小军
Owner HUNAN NORMAL UNIVERSITY
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