Constructing method of transcription expression gene model of ultraconserved region of peripheral blood mononuclear cells of systemic lupus erythematosus, and applications of the transcription expression gene model

An ultra-conserved region, lupus erythematosus technology, applied in blood/immune system cells, animal cells, vertebrate cells, etc.

Active Publication Date: 2014-07-09
中国人民解放军联勤保障部队第九二四医院
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[0004] However, T-UCRs have not been studied in SLE

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  • Constructing method of transcription expression gene model of ultraconserved region of peripheral blood mononuclear cells of systemic lupus erythematosus, and applications of the transcription expression gene model
  • Constructing method of transcription expression gene model of ultraconserved region of peripheral blood mononuclear cells of systemic lupus erythematosus, and applications of the transcription expression gene model
  • Constructing method of transcription expression gene model of ultraconserved region of peripheral blood mononuclear cells of systemic lupus erythematosus, and applications of the transcription expression gene model

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Embodiment Construction

[0018] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described in this specification. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0019] Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used in the description of the present invention in this specification are only for the purpose of describing specific embodiments, and are not used to limit the present invention. The term ...

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Abstract

The invention relates to a constructing method of a transcription expression gene model of a ultraconserved region of peripheral blood mononuclear cells of systemic lupus erythematosus, and applications of the transcription expression gene model. The constructing method includes: a step of setting an SLE group and a normal control with each group comprising a plurality of whole blood samples, and separating to obtain peripheral blood mononuclear cells; a step of measuring the content, the quality and the integrity of the sample RNA and determining that the sample RNA can be used for the next step; a step of analyzing the T-UCRs expression level of human bodies by adoption of a T-UCR chip, and detecting UCRs labeled by a gene specific probe; a step of performing RNA labeling and chip hybridization; a step of analyzing the chip scanning results by a feature extraction method; a step of indentifying differentially-expressed RNA transcriptons of latent T-UCRs and overlapping UCRs of the latent T-UCRs by adoption of FC filtration, and measuring the functions of the differential mRNA related to the UCRs in biological processes by adoption of GO analysis; and a step of comparing changes of the multiple among the samples in the two group and screening the differentially-expressed RNA.

Description

【Technical field】 [0001] The invention relates to the field of systemic lupus erythematosus, in particular to a construction method and application of a transcriptional expression gene model of an ultra-conserved region of peripheral blood mononuclear cells of systemic lupus erythematosus. 【Background technique】 [0002] Systemic lupus erythematosus (SLE) is a typical systemic autoimmune disease associated with diffuse connective tissue, and its important feature is immune inflammation. The pathogenesis of SLE is complex, involving family genetics, immune and environmental factors, etc., which may lead to tissue and organ damage, the most complicated of which is renal function damage. [0003] Recent studies have found that the extremely conserved long non-coding gene regions in human, mouse and rat genes are named ultra-conserved regions (UCRs). UCR is a sequence about 200-779bp in length, which was first discovered by Bejerano in 2004. UCRs are often located in tumor-ass...

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Application Information

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IPC IPC(8): C12Q1/68C12N5/0786
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 眭维国戴勇曹翠辉薛雯
Owner 中国人民解放军联勤保障部队第九二四医院
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