Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for identifying Wuzhishan miniature pig inbred line by applying SNP (single nucleotide polymorphism) of MLANA gene

A technology for miniature pigs and inbred lines, which is applied in the directions of DNA/RNA fragmentation, recombinant DNA technology, and the determination/inspection of microorganisms, and can solve problems such as the establishment standards and identification methods of non-inbred lines.

Active Publication Date: 2014-07-16
GRAND LIFE SCI & TECH
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since there is no report on the successful breeding of inbred pigs in the world, there is no standard and identification method for the establishment of inbred pigs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for identifying Wuzhishan miniature pig inbred line by applying SNP (single nucleotide polymorphism) of MLANA gene
  • Method for identifying Wuzhishan miniature pig inbred line by applying SNP (single nucleotide polymorphism) of MLANA gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Discovery of the genetic law of the "never homozygous" gene

[0027] Using the Illumina pig high-density SNP chip (60,000 SNPs), F in Wuzhishan minipig inbred lines 17 to F 22 A total of 6 generations and a total of 112 samples of Hainan Wuzhishan pig were genotyped systematically.

[0028] 1. Homozygosity analysis

[0029] Among the 61,565 effective SNPs distributed on 18 pairs of autosomes of the Wuzhishan minipig inbred line, F 20 The proportion of SNPs that has been fixed is as high as 80.0%. With the advancement of generations, some regions on almost all chromosomes are slowly fixed, and the heterozygosity is from large to small until it is 0, and these regions change with generations. The propulsion tends to become larger, and these phenomena are in line with the unique genetic laws of inbred animals. Almost all chromosomal regions of Hainan Wuzhishan pig population were highly heterozygous, and 61565 effective SNPs were diffusely, uniformly and irr...

Embodiment 2

[0033] Example 2, the analysis of the heterozygosity of 1 locus, and further verification of the "never homozygous" gene

[0034] The analysis found that 7646 SNP sites in F 20 The above are still heterozygous, and these SNP sites can be mapped to 2238 genes. The analysis results show that more than 80% of the genes are involved in metabolic process (750 genes in total, accounting for 45%) and cellular process (643 genes in total, accounting for 38.6%), these genes are necessary to maintain life activities, Consistent results with whole genome sequencing. The reason for not being homozygous by the inbreeding process is revealed again.

[0035] Further analysis of the heterozygous SNP sites found that the inbred line had a site with a heterozygosity of 1, but it was not observed in the corresponding Hainan Wuzhishan pig site, and this phenomenon was observed in each inbred line. There is still an irregular and mosaic-like change trend in the cross-generation, that is: F 17 ...

Embodiment 3

[0037] Example 3. Identification of Wuzhishan Miniature Pig Inbred Lines and Non-Wuzhishan Miniature Pig Inbred Lines Using Never Homozygous MLANA Gene

[0038] Function of MLANA gene: involved in cellular immunity. The MLANA gene is located on chromosome 1, and the SNP involved in the present invention is located at the 241959813 (Sscrofa10.2) site on chromosome 1 (the 206th nucleotide from the 5' end of sequence 1 in the sequence table), which is an A / G mutation .

[0039] The sample to be tested is F from the inbred strain of Wuzhishan miniature pigs 17 to F 22 A total of 96 pigs from 6 generations (16 pigs per generation), and 34 Hainan Wuzhishan pigs. Each sample to be tested is carried out as follows:

[0040] 1. Take a blood sample and extract genomic DNA from the blood sample.

[0041] 2. Using the genomic DNA extracted in step 1 as a template, perform PCR amplification with a primer pair composed of F1 and R1 (the annealing temperature is 55° C.), and obtain a PC...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for identifying a Wuzhishan miniature pig inbred line by applying SNP (single nucleotide polymorphism) of an MLANA gene. The invention provides a method for identifying whether the pig population to be tested is the Wuzhishan miniature pig inbred line population in an auxiliary manner. The method comprises the following steps of obtaining samples to be tested through random sampling from the pig population to be tested, then detecting the genotypes of ALGA0007899SNP sites based on the MLANA genes of the samples to be tested, determining the pig population to be tested to be the candidate Wuzhishan miniature pig inbred line population if all the samples to be tested are hybrid, and determining the pig population to be tested to be the candidate non-Wuzhishan miniature pig inbred line population if more than one of all the samples to be tested is homozygous. The method is of great value to identification of germplasm resources of the Wuzhishan miniature pig inbred line.

Description

technical field [0001] The invention relates to a method for identifying Wuzhishan miniature pig inbred lines by using SNP of MLANA gene. Background technique [0002] From 1989 to 2003, "Wuzhishan Inbred Line Cultivation, Germplasm Characteristics and Development and Utilization" was included in the Ministry of Agriculture's "Seventh Five-Year, Eighth Five-Year, and Ninth Five-Year Plan" industry research projects, as well as the Ministry of Science and Technology's "863" and the National Natural Science Foundation of China. With the support of 7 subjects, including 1 male and 1 female Wuzhishan pigs (also known as Wuzhishan miniature pigs) as the ancestors, the continuous use of "little pigs mating mothers", "full compatriots" "Comprehensive measures such as mating, and gradually overcome the adverse effects of inbreeding, which leads to extremely low reproductive survival rates and less than 20%, and established F 17 The population and pedigree of the inbred strain were ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 冯书堂牟玉莲李奎吴添文刘岚黄雷
Owner GRAND LIFE SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com