Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Secondary utilization method of rooting medium for stevia rebaudiana

A rooting medium and rooting culture technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as insufficient utilization of nutrients, and achieve the effects of reducing consumption and increasing income

Inactive Publication Date: 2014-07-23
有限会社林平
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to solve the problem of insufficient utilization of nutrients in the culture medium of stevia rooting seedlings, and propose a secondary method of stevia rooting medium How to use

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Secondary utilization method of rooting medium for stevia rebaudiana

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0013] An example of repeated utilization of rooting medium for three strains of Stevia rebaudiana

[0014] Carry out rooting culture to 3 Stevia rebaudiana lines B1, +3, 4, culture condition: culture medium

[0015] 1 / 2MS rooting medium, temperature 24°C, light 2100lux / cm 2 , adopt 250ml culture bottle, inoculate 5 Stevia rebaudiana stem segments in each bottle, 10 bottles of each line, cultivate for 30 days. When the first batch of rooted shoots was cultivated to 30 days, the rooted shoots of the first crop were pulled out from the bottle under the aseptic condition of the ultra-clean workbench, and then 5 new rooted shoots were inserted into the residual medium in each culture bottle. The Stevia rebaudiana stem section was cultured for 30 days in a second crop. When the period expires, the rooted seedlings are pulled out in the same way, and the stem segment of the third crop is inserted, and cultivated for 30 days.

[0016] investigation method:

[0017] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a secondary utilization method of a rooting medium for stevia rebaudiana. After tissue cultured rooted seedlings of stevia rebaudiana are cultured, the rooted seedlings are pulled out of a culture bottle, and single-node stems of stevia rebaudiana are reconnected to an original medium to be subjected to secondary rooting culture, so that residual nutrient substances in the medium can be fully used, and a crop of rooted seedlings can be re-obtained. The measure can save the tissue culture cost of stevia rebaudiana, thereby improving the economic benefits.

Description

technical field [0001] The invention relates to the technical field of stevia rebaudiana tissue culture, in particular to a method for secondary utilization of stevia rebaudiana rooting medium. Background technique [0002] Stevia tissue culture is an important platform for the rapid propagation of stevia and a series of scientific research. Stevia rebaudiana is rooted and cultured, and the inoculated stem section grows into a rooted stevia rooted seedling plant with complete roots, stems and leaves. In order to expand the multiple of reproduction, it is usually pulled out from the culture medium after the branches of the rooted seedling are full of culture bottles, and then The branches were cut into single-node stem segments for outdoor cutting and seedling cultivation, and the culture medium was disposed of as waste. However, according to our analysis, the culture medium in the tissue culture bottle has undergone a rooting culture, and the nutrients therein have not all ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 林平丁雪丰何克勤
Owner 有限会社林平
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com