Method for long-term storage of crassostrea hongkongensis eyespot larvas by critical low temperatures before freezing point

A technology for long-term preservation of Hong Kong oysters, applied in the fields of application, fish farming, climate change adaptation, etc., can solve the problems of long-term preservation of Hong Kong oyster eye spot larvae and other problems that have not yet been seen, and achieves strong practicability, easy operation, and improved seedling harvesting. The effect of efficiency

Active Publication Date: 2014-07-23
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

Although there are individual reports on the short-term preservation of oyster larvae at low temperature (Yu Rui

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] a. Larvae collection: On May 30, 2010, in the Dalian Zhuanghe marine shellfish nursery, 60m of larvae were concentrated using 80 sieve silk nets (the maximum aperture of the mesh is 250μm). 3 A total of 60 million eye-spot larvae were obtained from the Hong Kong oyster eye-spot larvae in the cement pond, and the shell height was 320-360 μm.

[0021] b. Refrigeration of larvae: pack the eye-spot larvae in step a with 500-mesh sieve tulle (the maximum aperture of the mesh is 20 μm) and pack them into bags, each bag ≤ 1kg, and then wrap the outside with a sponge moistened with seawater One layer, put it in a 2L incubator and seal it, and then place it in a refrigerator at 0-1°C to refrigerate.

[0022] c. Larvae management: Spray seawater on the outer layer of the sponge with a small sprayer every 7 days for the eye-spot larvae packaged in step b, so that it is always in a moist state.

[0023] d. Thawing larvae: After refrigerated for 60 days, take it out of the refriger...

Embodiment 2

[0025] a. Larvae collection: On June 10, 2011, in the nursery of Shenzhen Xulian Marine Biology Co., Ltd., 30m of larvae were concentrated using 80 sieve silk nets (the maximum aperture of the mesh is 250μm). 3 A total of 15 million eye-spot larvae were obtained from the Hong Kong oyster eye-spot larvae in the cement pond, and the shell height was 320-360 μm.

[0026] b. Refrigeration of larvae: pack the eye-spot larvae in step a with 500-mesh sieve tulle (the maximum aperture of the mesh is 20 μm) and pack them into bags, each bag ≤ 1kg, and then wrap the outside with a sponge moistened with seawater One layer, put it in a 2L incubator and seal it, and then place it in a refrigerator at 0-1°C to refrigerate.

[0027] c. Larvae management: Spray seawater on the outer layer of the sponge with a small sprayer every 7 days for the eye-spot larvae packaged in step b, so that it is always in a moist state.

[0028] d. Thawing larvae: After refrigerating for 36 days, take it out of...

Embodiment 3

[0030] a. Larvae collection: On June 15, 2012, in the Zhanjiang Marine Economic Animal Experimental Nursery of the South China Sea Institute of Oceanology, Chinese Academy of Sciences, 20m was concentrated using 80 sieve silk nets (the maximum aperture of the mesh was 250μm). 3 A total of 10 million eye-spot larvae were obtained from Hong Kong oyster eye-spot larvae in cement ponds, and the shell height was 320-360 μm.

[0031] b. Refrigeration of larvae: pack the eye-spot larvae in step a with 500-mesh sieve tulle (the maximum aperture of the mesh is 20 μm) and pack them into bags, each bag ≤ 1kg, and then wrap the outside with a sponge moistened with seawater One layer, put it in a 2L incubator and seal it, and then place it in a refrigerator at 0-1°C to refrigerate.

[0032] c. Larvae management: Spray seawater on the outer layer of the sponge with a small sprayer every 7 days for the eye-spot larvae packaged in step b, so that it is always in a moist state.

[0033] d. Th...

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Abstract

The invention dislcoses a method for long-term storage of crassostrea hongkongensis eyespot larvas by critical low temperatures before the freezing point. The method comprises the steps that a, the larvas are stored in a refrigeration mode, the concentrated crassostrea hongkongensis eyespot larvas are collected and are packaged and bundled into packages by bolting-silk meshes which is wetted by seawater, and then the packages are wrapped with sponges wetted by seawater and are placed at the temperature of 0-1 DEG C for refrigeration; b, according to the refrigerated eyespot larvas in the step a, seawater is sprayed on the sponges, and the eyespot larvas are made to keep wet; c, the larvas are unfrozen, the refrigerated eyespot larvas in the step b are taken out from the low temperature environments and are wetted by normal-temperature seawater, then the larvas are put in a container and evenly stirred, and the eyespot larvas can be obtained after the larvas are completely restored to be at the normal temperature. The eyespot larvas stored in the method almost do not suffer from frostbite, the seedling collecting efficiency can be effectively improved, and 90% and more of the eyespot larvas can achieve metamorphosis. The method provides a theory and practice foundation for studying cryobiology of the crassostrea hongkongensis larvas, dry larvas transportation, large-scale seedling synchronous production and the like. The method for long-term storage of the crassostrea hongkongensis eyespot larvas by the critical low temperatures before the freezing point has the advantages that operation is simple and convenient, and the practicability is high.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish seed breeding in modern marine agriculture, and in particular relates to a method for long-term preservation of Hong Kong oyster eye-spot larvae by using a critical low temperature before freezing point. Background technique: [0002] Cryobiology is a new discipline developed in the 1950s. At present, this discipline has been widely used in the preservation of gametes and embryos of humans and animals (Chang Wancun et al., 1998). Although ultra-low temperature freezing technology has been widely used in marine organisms, because the eggs of some marine organisms are relatively large, and contain a large number of yolks, oil droplets, etc., which increases the difficulty of cryopreservation, research in this area has focused on marine life. Research on cryopreservation of biological sperm (Li Yongren et al., 2005). [0003] At present, the cryopreservation sperm technology has been successfully...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 张跃环肖述张扬李军向志明喻子牛
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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