A method for inducing diapause of budworm

A technology of budworm and diapause, which is applied in animal husbandry and other fields, to achieve good effect, convenient operation and shorten the overall lifespan

Active Publication Date: 2016-08-17
JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although some insects have experienced and released diapause, if they are affected by environmental factors again, they will enter diapause again

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The newly hatched larvae of P. tabaci were raised in a 10cm-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 20°C, the light-to-dark ratio was 10:14, and the light intensity was 33%. . Then move the worms to an artificial climate box at 4°C with a relative humidity of 78% for 10 days, then place them in a refrigerator at 0°C for 15 days, repeat the treatment twice, and finally move the treated worms to 25°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.

Embodiment 2

[0020] The newly hatched larvae of P. tabaci were raised in a 15m-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 25°C, the light-to-dark ratio was 10:14, and the light intensity was 31%. . Then move the worms to 4°C, 86% relative humidity in an artificial climate box for 10 days, then place them in a refrigerator at 0°C for 15 days, repeat the treatment 3 times, and finally move the treated worms to 32°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.

Embodiment 3

[0022] The newly hatched larvae of P. tabacum were raised in a 13m-diameter petri dish with artificial feed until the third instar, and then raised in a 1.2cm×10cm glass test tube. The temperature was pre-controlled at 21°C, the light-to-dark ratio was 10:14, and the light intensity was 29%. . Then move the worms to 4°C, 80% relative humidity in an artificial climate box for 10 days, then put them in a refrigerator at 0°C for 15 days, repeat the treatment 3 times, and finally move the treated worms to 27°C Process 3d. Then cultivate normally, and then the diapause of P. tabaci can be made.

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PUM

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Abstract

The invention provides a method for inducing diapause of oriental tobacco budworms, and belongs to the technical field of insect pest prevention and control. The method comprises the following steps that (1) newly hatched larvae of the oriental tobacco budworms are bred to three ages in a culture dish with the diameter being 10-15 cm by artificial feed, then the oriental tobacco budworms are bred in a 1.2cm*10cm glass test tube, the temperature is pre-controlled to be 20-25 DEG C, and the light dark ratio is 8-11: 13-16, the light intensity is 25-35%, and the budworms are in standby application; (2) the budworms in the (1) step are transferred to a manual climatic box to be bred for 10-12 days, the temperature of the manual climatic box is 4-5 DEG C, the relative humidity of the manual climatic box is 78-86%, then the budworms are bred in a 0 DEG C refrigerator for 15-18 days, processing is repeated two to three times, and standby application is conducted; (3) at last, the budworms which are processed in the (2) step are transferred to a 25-32 DEG C box to be bred for three to four days, then normal breeding is conducted, and diapause of the oriental tobacco budworms can be achieved. According to the method for inducing diapause of the oriental tobacco budworms, operation is convenient, cost is low, the effect is good, and any bad effect can not happen.

Description

technical field [0001] The invention belongs to the technical field of pest control, and in particular relates to a method for inducing diapause of the tobacco budworm. Background technique [0002] Tobacco budworm, also known as tobacco budworm or tobacco spider moth, belongs to Lepidoptera and Noctuidae in classification. Tobacco budworm has a wide distribution, miscellaneous feeding habits, strong fecundity, strong stress resistance, and diapause characteristics, and is the most serious harm to tobacco. In recent years, serious hazards have occurred in many tobacco producing areas, such as Guizhou, Sichuan, Central China and Huanghuai tobacco areas. Hazarded by budworms, tobacco yields will lose 5%-10% in normal years, and more than 15% in major years. The harm of the tobacco budworm is caused by its larvae feeding on the leaves, flowers, buds, fruits, etc. of the host, so the larval age and generation number directly affect the degree of damage to crops. Due to variou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
Inventor 李兴彪
Owner JIYANG COLLEGE OF ZHEJIANG A & F UNIV
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