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A low-energy ion implantation protective agent and its application

A technology of ion implantation and protective agent, which is applied in the direction of electric/wave energy treatment of enzymes, electric/wave energy treatment of microorganisms, microorganisms, etc., can solve the problems of difficult survival of microorganisms, high mortality, and reduce mortality, so as to improve survival rate effect

Active Publication Date: 2015-09-30
源创吉因(重庆)细胞应用技术研究院有限公司 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ion implantation needs to be completely dry and under the condition of a vacuum of 0.06 Pa. Most microorganisms are difficult to withstand such an extreme environment, and the mortality rate is very high. Even some microorganisms are difficult to survive, and effective protective agents are needed to reduce the mortality rate.

Method used

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  • A low-energy ion implantation protective agent and its application
  • A low-energy ion implantation protective agent and its application
  • A low-energy ion implantation protective agent and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment 1, the protective effect of protective agent on Lactobacillus rhamnosus (Lactobacillus rhamnosus JCM1136)

[0053] The basic protective solution used in the preparation of protective agent 1, protective agent 2 and protective agent 3 in this example is composed of solvent and solute, the solvent is distilled water, and the solute is trehalose, histidine, sodium tripolyphosphate, sodium glutamate The concentration of the trehalose in the basic protection solution is 3.0g / 100ml, the concentration of the histidine in the basic protection solution is 1.6g / 100ml, and the sodium tripolyphosphate is in the basic protection solution The concentration in the protection solution is 1.3g / 100ml, the concentration of the sodium glutamate in the basic protection solution is 0.5g / 100ml, and the pH value of the solution is adjusted with an aqueous solution of 1% hydrochloric acid by volume to 6.0.

[0054] Protection test:

[0055] Lactobacillus rhamnosus JCM1136 (Lactobac...

Embodiment 2

[0065] Embodiment 2, the protective effect of protective agent on plant Lactobacillus subsp. (Lactobacillus plantarum subsp.plantarum JCM1149)

[0066] The basic protective solution used in the preparation of protective agent 1, protective agent 2 and protective agent 3 in this example is composed of solvent and solute, the solvent is distilled water, and the solute is trehalose, histidine, sodium tripolyphosphate, sodium glutamate The concentration of the trehalose in the basic protection solution is 2.0g / 100ml, the concentration of the histidine in the basic protection solution is 1.8g / 100ml, and the sodium tripolyphosphate is in the basic protection solution The concentration in the protective solution is 1.6g / 100ml, the concentration of the sodium glutamate in the basic protective solution is 0.3g / 100ml, and the pH value of the solution is adjusted with an aqueous solution of 1% hydrochloric acid by volume to 6.0.

[0067] The protection test of the protective agent on La...

Embodiment 3

[0075] Embodiment 3, the protective effect of protective agent on Lactobacillus pentosus (Lactobacillus pentosus JCM1558)

[0076]The basic protective solution used in the preparation of protective agent 1, protective agent 2 and protective agent 3 in this example is composed of solvent and solute, the solvent is distilled water, and the solute is trehalose, histidine, sodium tripolyphosphate, sodium glutamate The concentration of the trehalose in the basic protection solution is 4.0g / 100ml, the concentration of the histidine in the basic protection solution is 1.4g / 100ml, and the sodium tripolyphosphate in the basic The concentration in the protective solution is 1.0g / 100ml, the concentration of the sodium glutamate in the basic protective solution is 0.8g / 100ml, and the pH value of the solution is adjusted with an aqueous solution of 1% hydrochloric acid by volume to 6.0.

[0077] The protection test of the protective agent on Lactobacillus pentosus JCM1558 is the same as t...

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Abstract

The invention discloses a low-energy ion implantation protectant and an application thereof. The protectant comprises a solvent and a solute, wherein the solvent is distilled water and the solute comprises trehalose, histidine, sodium tripolyphosphate, sodium glutamate and sodium hyaluronate. The protectant disclosed by the invention can significantly increase the survival quantity of the strain under the ion implantation conditions.

Description

technical field [0001] The invention relates to a protective agent for ion implantation in the field of biotechnology and its application, in particular to a low-energy ion implantation protective agent and its application. Background technique [0002] As a means of biological mutagenesis, ion implantation technology has attracted attention due to its unique mutagenesis mechanism and significant biological effects, and is used in plant and microbial breeding. Low-energy ion beams have a strong mutagenic effect on microorganisms, and have been successfully applied to the research of microbial mutation breeding, which has achieved huge economic and social benefits. However, ion implantation requires complete dryness and a vacuum of 0.06 Pa. Most microorganisms are difficult to withstand such an extreme environment, and the mortality rate is high. Even some microorganisms are difficult to survive, and effective protective agents are needed to reduce the mortality rate. Conte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00
Inventor 谈重芳苏明杰庞会利焦浈王雁萍李宗伟
Owner 源创吉因(重庆)细胞应用技术研究院有限公司