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Tissue regeneration guiding membrane and preparation method thereof

A tissue regeneration and guiding membrane technology, applied in medical science, surgery, etc., can solve the problems of mismatched bone tissue growth rate, difficulty in large-scale production, and easy collapse of the structure in contact with water, so as to reduce medical costs and reduce raw material sources. , good biocompatibility

Active Publication Date: 2016-01-20
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its poor mechanical properties, the structure is easy to collapse when exposed to water, degrades too fast, and does not match the growth rate of bone tissue.
In order to overcome the defects of collagen material degradation, chemical cross-linking agents such as glutaraldehyde are also used to prolong its degradation time in the body, but their residue in the product will cause the patient's body to reject the product, and its preparation method is complicated. Therefore, large-scale production is difficult

Method used

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  • Tissue regeneration guiding membrane and preparation method thereof

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preparation example Construction

[0029] Such as figure 1 As shown, the present invention provides a method for preparing a tissue regeneration guiding membrane, comprising the following steps:

[0030] 1. Preprocessing steps

[0031] Collect the peritoneum of mammals, wash with running water and depilate, and then use the mechanical method of running water to wash for preliminary degreasing, and then store them in an environment of -20-4°C for later use; in this step, pigs, cattle or sheep that have just been slaughtered are preferably collected peritoneum, especially in young pigs aged 6-7 weeks. The natural collagen membrane of mammals has a smooth surface and a fibrous surface, and the corresponding extracted collagen matrix is ​​isolated not only from the surrounding connective tissue in vivo, but also from cartilage defects.

[0032] Since the location of the subsequent processing step and the slaughterhouse are generally not at the same location, the peritoneum is usually transported from the slaughte...

Embodiment 1

[0060] Step 1: Raw material pretreatment

[0061] The peritoneum is taken from the pig peritoneum of 6-7 weeks old, stored and transported at 4°C to the place where the guide membrane is processed, the surface blood and muscle tissue are removed, the hair is removed, the fat is removed, and the running water is washed for half an hour. Wash with salt water shock 3 times, 15 minutes each time.

[0062] Step 2: Degrease

[0063] First immerse the peritoneum in a mixture containing 1% sodium bicarbonate and 0.1% lipase and stir evenly for 10 hours, then stir evenly with 0.2% pepsin solution for 10 hours, the whole process Maintain pH=7 at room temperature with 2-4 rinses in between.

[0064] Step 3: Alkaline Treatment

[0065] The remaining fat on the peritoneum and the roots and follicles of the hair in the skin are removed. The peritoneum was treated in 0.3% NaOH solution for 12 hours, and the membrane needed to be agitated well during the treatment. Then, the alkali is wa...

Embodiment 2

[0074] Step 1: Raw material pretreatment

[0075] The peritoneum is taken from the peritoneum of 2-3 months old cattle, stored at -20°C and transported to the processing site of the guide membrane. The surface blood and muscle tissue are removed, the hair is removed, and the fat is removed. After half an hour of running water, rinse with normal saline. Shake and wash 3 times, 15 minutes each time.

[0076] Step 2: Degrease

[0077] The peritoneum was immersed in a mixture containing 1% sodium bicarbonate and 1% lipase and stirred evenly for 5 hours, and then stirred evenly with a 2% neutral protease solution for 3 hours. The process maintained pH=7 at room temperature, with 2-4 rinses in between.

[0078] Step 3: Alkaline Treatment

[0079] The remaining fat on the peritoneum and the roots and follicles of the hair in the skin are removed. Treat the peritoneum in a 5% NaOH solution by mass for 10 hours, and the membrane needs to be agitated well during the treatment. Then...

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Abstract

The invention relates to a guided tissue regeneration membrane and a preparation method thereof. The method comprises: a pretreatment step, i.e. collecting a mammalian peritoneum, conducting flowing water washing, hair removal and preliminary degreasing for standby use; an antigen removal treatment step, i.e. carrying out further degreasing on the standby peritoneum by means of an enzyme treatment process, an alkali treatment process, an acid treatment process and a hypertonic treatment process, and removing immunogenicity; a collagen fiber contraction treatment step, i.e. carrying out dehydration, non-hydrophilic substance removal and washing treatment on the peritoneum subjected to the antigen removal treatment so as to contract the collagen fibers of the peritoneum; and a drying step, spreading the contracted peritoneum on a culture dish surface to make the compact layer of the peritoneum contact the culture dish, rapidly transferring the peritoneum into a refrigerator at a temperature ranging from -60 to -80DEG C to undergo pre-freezing for 1-8h, then freeze-drying the peritoneums in a freeze dryer for 24-36h, thus obtaining a collagen membrane able to guide tissue regeneration. The guided membrane provided by the invention has a compact layer and a loose layer, also has good biocompatibility, hydrophilicity and bone induction ability, and has a long in-vivo degradation time.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a tissue regeneration guiding membrane and a preparation method thereof. Background technique [0002] Guided tissue regeneration (GTR) has been widely used in the treatment of severe periodontal disease. GTR membrane materials can be divided into non-absorbable membrane materials and absorbable membrane materials. [0003] Non-absorbable membrane materials (such as GTR membranes made of PTFE) are extremely painful for patients because they must be removed by secondary surgery, and cannot be used for regeneration of larger defect areas because they are not bioactive , Therefore, most of the non-degradable and absorbable membrane materials on the market have basically been eliminated. [0004] At present, there are few degradable and absorbable tissue regeneration guiding membrane products on the market, and all of them have some defects. The GTR membrane made of co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/12A61L31/04A61L31/14
Inventor 谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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