Tissue regeneration guiding membrane and preparation method thereof
A tissue regeneration and guiding membrane technology, applied in medical science, surgery, etc., can solve the problems of mismatched bone tissue growth rate, difficulty in large-scale production, and easy collapse of the structure in contact with water, so as to reduce medical costs and reduce raw material sources. , good biocompatibility
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[0029] Such as figure 1 As shown, the present invention provides a method for preparing a tissue regeneration guiding membrane, comprising the following steps:
[0030] 1. Preprocessing steps
[0031] Collect the peritoneum of mammals, wash with running water and depilate, and then use the mechanical method of running water to wash for preliminary degreasing, and then store them in an environment of -20-4°C for later use; in this step, pigs, cattle or sheep that have just been slaughtered are preferably collected peritoneum, especially in young pigs aged 6-7 weeks. The natural collagen membrane of mammals has a smooth surface and a fibrous surface, and the corresponding extracted collagen matrix is isolated not only from the surrounding connective tissue in vivo, but also from cartilage defects.
[0032] Since the location of the subsequent processing step and the slaughterhouse are generally not at the same location, the peritoneum is usually transported from the slaughte...
Embodiment 1
[0060] Step 1: Raw material pretreatment
[0061] The peritoneum is taken from the pig peritoneum of 6-7 weeks old, stored and transported at 4°C to the place where the guide membrane is processed, the surface blood and muscle tissue are removed, the hair is removed, the fat is removed, and the running water is washed for half an hour. Wash with salt water shock 3 times, 15 minutes each time.
[0062] Step 2: Degrease
[0063] First immerse the peritoneum in a mixture containing 1% sodium bicarbonate and 0.1% lipase and stir evenly for 10 hours, then stir evenly with 0.2% pepsin solution for 10 hours, the whole process Maintain pH=7 at room temperature with 2-4 rinses in between.
[0064] Step 3: Alkaline Treatment
[0065] The remaining fat on the peritoneum and the roots and follicles of the hair in the skin are removed. The peritoneum was treated in 0.3% NaOH solution for 12 hours, and the membrane needed to be agitated well during the treatment. Then, the alkali is wa...
Embodiment 2
[0074] Step 1: Raw material pretreatment
[0075] The peritoneum is taken from the peritoneum of 2-3 months old cattle, stored at -20°C and transported to the processing site of the guide membrane. The surface blood and muscle tissue are removed, the hair is removed, and the fat is removed. After half an hour of running water, rinse with normal saline. Shake and wash 3 times, 15 minutes each time.
[0076] Step 2: Degrease
[0077] The peritoneum was immersed in a mixture containing 1% sodium bicarbonate and 1% lipase and stirred evenly for 5 hours, and then stirred evenly with a 2% neutral protease solution for 3 hours. The process maintained pH=7 at room temperature, with 2-4 rinses in between.
[0078] Step 3: Alkaline Treatment
[0079] The remaining fat on the peritoneum and the roots and follicles of the hair in the skin are removed. Treat the peritoneum in a 5% NaOH solution by mass for 10 hours, and the membrane needs to be agitated well during the treatment. Then...
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