32results about How to "Raise the isoelectric point" patented technology

The invention relates to a preparation method of an efficient calcium-based sepiolite phosphorus adsorbent. The preparation method comprises the following steps of mechanically pulverizing natural calcium-enriched sepiolite ore into powder, drying the powder at constant temperature, and then directly heating and modifying the powder to obtain the calcium-based sepiolite phosphorus adsorbent. According to the preparation method provided by the invention, inertia calcium in the natural calcium-enriched sepiolite is activated through a heating and modification method, a part of active calcium enters into a sepiolite lattice system, and the alcium-based sepiolite adsorbent having high absorption capacity to phosphorus is generated by the original ore through recombination and reaction. The calcium-based sepiolite phosphorus adsorbent provided by the invention is used for eliminating phosphorus in polluted water or bottom sediment, and is capable of efficiently and rapidly eliminating phosphate in the water and efficiently translating labile phosphorus in the polluted bottom sediment into inert calcium phosphorus, thereby achieving the aim of controlling nutrition enrichment of lakes. The calcium-based sepiolite phosphorus adsorbent prepared by the preparation method has the advantages of wide raw material distribution, low cost, simple preparation process, obvious phosphorus fixation effect, and wide application prospect and use value.

The invention relates to a guided tissue regeneration membrane and a preparation method thereof. The method comprises: a pretreatment step, i.e. collecting a mammalian peritoneum, conducting flowing water washing, hair removal and preliminary degreasing for standby use; an antigen removal treatment step, i.e. carrying out further degreasing on the standby peritoneum by means of an enzyme treatment process, an alkali treatment process, an acid treatment process and a hypertonic treatment process, and removing immunogenicity; a collagen fiber contraction treatment step, i.e. carrying out dehydration, non-hydrophilic substance removal and washing treatment on the peritoneum subjected to the antigen removal treatment so as to contract the collagen fibers of the peritoneum; and a drying step, spreading the contracted peritoneum on a culture dish surface to make the compact layer of the peritoneum contact the culture dish, rapidly transferring the peritoneum into a refrigerator at a temperature ranging from -60 to -80DEG C to undergo pre-freezing for 1-8h, then freeze-drying the peritoneums in a freeze dryer for 24-36h, thus obtaining a collagen membrane able to guide tissue regeneration. The guided membrane provided by the invention has a compact layer and a loose layer, also has good biocompatibility, hydrophilicity and bone induction ability, and has a long in-vivo degradation time.

The invention provides a light modified filtering material, a preparation method and application of the light modified filtering material. The light modified filtering material is prepared from the following components in parts by weight: 75-100 parts of waste glass, 5-20 parts of a metal oxide modifier and 1-10 parts of a foaming agent. The light modified filtering material has the advantages oflight weight, large surface area, high isoelectric point, porosity, and the like, the isoelectric point of the filtering material is added and the service life of the filtering material is prolonged.According to the preparation method, the metal oxide is added for combining with SiO2 in the glass and forming Si-O-Me (Me metal ion) which enters a glass network so as to increase the degree of polymerization of the structure, so that the glass isoelectric point is added while the toughness and hardness of the glass are promoted, the service life of the foam glass filtering material is prolongedand the practicability and economical benefit are promoted.

The invention discloses a fusion protein and a method for preparing a semeglutide intermediate polypeptide from the fusion protein, and belongs to the technical field of gene engineering and polypeptide preparation. The fusion protein comprises a segment of fusion peptide, a protease restriction enzyme cutting site and a target main molecule sequence. By optimizing the sequence of the fusion peptide and changing the isoelectric point, hydrophilicity and other properties of the protein, the expression quantity of the fusion protein is effectively improved, and the highest expression quantity can reach 13.1 g/L; meanwhile, the character of the fusion protein is also improved, the development of subsequent extraction, enzyme digestion and purification processes is facilitated, and the amount of the intermediate polypeptide after enzyme digestion is 3.62 g/L. The production cost of the semeglutide intermediate polypeptide Arg34GLP-1 (9-37) is reduced from the source, and the method is beneficial to industrial amplification and suitable for industrial production.

The invention discloses a packaged noble metal catalyst for liquid-phase catalytic hydrogenation reduction of Cr (VI) under a neutral condition and a preparation method of the packaged noble metal catalyst. The preparation method comprises the following steps: by taking mesoporous silica SBA-15 as a template, synchronously filling pores of SBA-15 with a carbon source aniline and a Pt source chloroplatinic acid, roasting, carbonizing and reducing noble metal platinum, carbonizing aniline to obtain a nitrogen-doped carbon skeleton, and removing the SBA-15 template to obtain the encapsulated noble metal catalyst. According to the catalyst, precious metal platinum is encapsulated in the nitrogen-doped ordered mesoporous carbon skeleton, the activity can be improved through nitrogen doping, small Pt particles and large-area catalytic active sites, the stability can be improved through an encapsulation type structure, and the catalyst has high activity and stability in Cr (VI) liquid-phase catalytic hydrogenation reduction reaction under the neutral condition.

The invention relates to a preparation method of a magnetic flocculant and application of the magnetic flocculant in removal of microcystis aeruginosa, the magnetic flocculant is prepared by the steps of setting environment temperature, regulating and controlling a Fe < 2 + >/Fe < 3 + > mixed solution, magnetically stirring and homogenizing and the like, and the magnetic flocculant is used for flocculation and magnetic separation of the microcystis aeruginosa. The magnetic flocculant is easy to prepare, high in flocculation efficiency and reusable, and can efficiently remove microcystis aeruginosa in water within 5 minutes.

The invention discloses a chrome-free tanning process for ecological furs. The chrome-free tanning process is characterized in that a'TWS + sodium lignin sulfonate + TWLZ 'ternary combined tanning process system is adopted. Sodium lignin sulfonate has a fixing effect on the TWS tanning agent, so that the TWS tanning agent cannot be subjected to detanning under a low pH condition, and the dispersion effect of the TWS tanning agent on collagenous fibers is enhanced, so that the TWLZ tanning agent can more uniformly permeate and be stably combined in a leather plate, and the defects of low isoelectric point and poor fullness of tanned furs of an existing TWS tanning system are effectively overcome; the fur which is full and uniform in tanning, high in isoelectric point and good in fullness is obtained, the application range of the chromium-free ecological fur is greatly widened, and development of the chromium-free tanning technology of the ecological fur is promoted.

The invention discloses a preparation method of a double-protein co-emulsified Pickering emulsion, and belongs to the technical field of food processing. The food-grade Pickering emulsion is preparedfrom soybean protein isolate nanoparticles and lactoferrin nanoparticles, so that the food-grade Pickering emulsion is more stable in the day and has excellent anti-aggregation stability. The preparation method provided by the invention is of great significance to design and manufacture of a formula of the protein-stabilized Pickering emulsion and even development of soybean protein products withcertain unique functions. In addition, 7s and 11s proteins in the soybean protein used in the method have good surface activity characteristics, so that the Pickering emulsion prepared by the method has good stability. The bovine lactoferrin is high in isoelectric point and is positively charged under a neutral condition, so that the bovine lactoferrin can interact with other biological macromolecules to improve the stability of the Pickering emulsion.

The present invention relates to a pharmaceutical preparation of a stable anti-human PD‑1 antibody, a combined drug and its application. The pharmaceutical preparation contains an anti-human PD‑1 monoclonal antibody, a stabilizer, a buffer and a surfactant. The pharmaceutical preparation of the anti-human PD‑1 antibody of the present invention can effectively inhibit the aggregation and deamidation of the antibody, thereby preventing the degradation of the antibody protein and obtaining a stable pharmaceutical preparation. The combined drug of the present invention is the combined use of the pharmaceutical preparation of the anti-human PD-1 antibody and other additional therapeutic agents, and the use of the pharmaceutical preparation or the combined drug in the preparation of anti-tumor drugs.

The invention belongs to the technical field of leather processing, and discloses an ecological leather chrome-free tanning process which is characterized in that a'TWS + sodium lignin sulfonate + TWLZ 'ternary tanning process system is adopted. By adding the sodium lignin sulfonate, a fixing effect is formed on the TWS tanning agent, so that the TWS tanning leather cannot be subjected to detanning under the condition of low pH, the distribution uniformity of the TWLZ tanning agent in the leather can be remarkably improved, the TWLZ tanning agent is promoted to form a stable cross-linked network structure in leather collagenous fibers, the defects of the tanning performance of an existing tanning system are effectively overcome, and the tanning performance of the tanning system is improved. And the leather which is uniform and sufficient in tanning, high in isoelectric point, strong in positive electricity, good in dyeing and finishing material absorption and soft in leather body is obtained, and the leather is endowed with wider application range and better quality.

The invention provides hydrophobic decorative base paper and a preparation method thereof, and belongs to the field of decorative paper, and the hydrophobic decorative base paper is prepared by using softwood pulp and hardwood pulp as main materials, using straw pulp and hemp pulp to replace a small part of wood pulp, and using chitosan and titanium dioxide as fillers. The hydrophobic decorative base paper is prepared through the procedures of repulping, pulping, pulp mixing, net part forming, squeezing, drying, surface sizing, secondary drying, calendaring, reeling, slitting, inspecting and warehousing, and the obtained hydrophobic decorative base paper is few in flaws, high in opaqueness, good in absorption performance, excellent in tensile strength, antibacterial and mildew-proof.

The invention belongs to the field of cosmetics, and particularly relates to a silicone oil-free shampoo composition with softening and repairing effects as well as a preparation method and application thereof. The silicone oil-free shampoo composition is prepared from the following components in parts by mass: 0.1-0.5 part of acrylamide propyl trimethyl ammonium chloride/acrylamide copolymer, 0.1-0.5 part of polyquaternium-10, 1-5 parts of PEG-75 shea butter glyceride, 1-5 parts of keratin and hydrolyzed keratin, and 0.5-2 parts of myristyl amide propane dimethyl ammonium chloride phosphate.According to the invention, the cationic surfactant is used to drive more polymers/oil flocculants to be adsorbed to damaged parts of hair, meanwhile, protein is tightly combined with the polyquaternium substance, the attachment strength of the protein on the hair is improved, so that as many beneficial components as possible are attached to the hair, and then the purpose of wetting, softening andbrightening the hair is achieved.

The invention discloses positively charged nano yttrium oxide and a preparation method thereof. The isoelectric point of nano Y2O3 with positive charges on the surface is 8-10, the Zeta potential of the surface is 20-30mV when the pH is 7, and the average size of the nano Y2O3 particles is 20-80nm. In the invention, an alcohol-water mixture is used as a reaction solvent, homogeneous nucleation of a yttrium-containing precursor is promoted by use of the solubility difference of the reactant in two solvents, and nano Y2O3 with relatively strong electropositivity and relatively high isoelectric point is obtained through high-temperature thermal decomposition. The method comprises the following specific steps: preparing an alcohol-water mixture according to a certain ratio as a solvent; separately adding a yttrium-containing compound and a precipitator; heating for reacting for homogeneous nucleation to obtain a yttrium-containing precursor; filtering the yttrium-containing precursor, washing and drying; and performing high-temperature thermal decomposition under the protection of a nitrogen atmosphere in a tube furnace to obtain surface positively-charged nano Y2O3. The invention is simple in technology, low in cost and suitable for industrial production.