The invention relates to organic polymer compound technical field, and provides a bi-color antibacterial EVA footwear and a preparation method thereof, two different colors of antibacterial EVA material grains are prepared from raw material EVA, ethylene-butene copolymer, 1, 2-polybutadiene, an antibacterial agent, chlorinated polyethylene, a foaming agent, a crosslinking agent, zinc stearate, stearic acid, a wear-resisting agent, talcum powder and a pigment by smelting, open milling and granulation, two different colors of antibacterial EVA material grains are respectively pouring into different material absorption barrels of an EVA injection machine table for once ejection to obtain the bi-color antibacterial EVA footwear, the echerichia coli and staphylococcus aureus antibacterial rates of the bi-color antibacterial EVA footwear are more than 99%, the bi-color antibacterial EVA footwear has good water-proof weather-proof lasting antibacterial properties, has the characteristics of light weight, good comprehensive performance, high production efficiency, high product qualified rate and the like, and is especially suitable for production of sandals, slippers, indoor shoes and other EVA shoes which are in direct contact with human skin, and is lightweight and comfortable, and cost-effective.