A Fluorescent Detection Method for Flavonoids in Hawthorn

A technology of fluorescence detection and flavonoids, applied in fluorescence/phosphorescence, material excitation analysis, preparation of test samples, etc., can solve the problems of single instrument selection and use, high cost of HPLC, large consumption of mobile phase, etc., to achieve application The effect of wide range, lower production cost, price breakthrough

Active Publication Date: 2016-04-20
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been many literature reports on the detection of flavonoids in hawthorn, but the detection methods and the selection and use of instruments are relatively simple, basically using high-performance liquid chromatography as the main detection system, but HPLC is expensive and requires various methods. Packed column, the mobile phase consumes a lot and is mostly toxic

Method used

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  • A Fluorescent Detection Method for Flavonoids in Hawthorn
  • A Fluorescent Detection Method for Flavonoids in Hawthorn
  • A Fluorescent Detection Method for Flavonoids in Hawthorn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1. The investigation of surfactant kind

[0048] 1.1 Take three clean 30ml jars, numbered 1, 2, and 3, and add 18mg of 3-sulfopropyl dodecyl dimethyl betaine to bottle No. 1, and add 18 mg of 3-sulfopropyl decathene to bottle No. Add 18 mg of 3-sulfopropylhexadecyl dimethyl betaine to tetraalkyl dimethyl betaine and No. 3 bottle, and then add 222 μL of n-butanol, 16.6 μL of ethyl acetate, and 750 μL of 20 mM borax to the three bottles Aqueous solution, 2.037ml of water, ultrasonic (100W, 40kHz) at 30°C for 20 minutes to prepare a microemulsion buffer solution.

[0049] 1.2 Take three 0.22μm organic membrane filters, corresponding to No. 1-3 syringes numbered 1, 2, and 3, and use the filter to filter the buffer solution in the syringe according to the corresponding number, one buffer solution corresponds to two A sample vial was loaded into a total of 6 sample vials. The buffer in the sample bottle is flushed into the capillary column as the buffer for capil...

Embodiment 2

[0054] Embodiment 2. Investigation of surfactant concentration

[0055] 2.1 Take 4 clean 30ml jars, numbered 1, 2, 3, 4, add 9mg of 3-sulfopropyl dodecyl dimethyl betaine and 2.046mL water into No. 1 bottle, add 18mg3 -Sulphopropyldodecyldimethylbetaine and 2.037mL of water, add 27mg of 3-sulfopropyldodecyldimethylbetaine and 2.028mL of water to bottle No. 3, add 36mg of 3-sulfopropyl betaine to bottle No. 4 Dodecyl dimethyl betaine and 2.019mL of water, and then add 222μL of n-butanol, 16.6μL of ethyl acetate, and 750μL of 20mM borax aqueous solution to four bottles, and ultrasonic (100W, 40kHz) at 30°C for 20 minutes. into a microemulsion buffer solution.

[0056] 2.2 Take four 0.22μm organic membrane filters, corresponding to No. 1-4 syringe numbers 1, 2, 3, 4, and use the filter to filter the buffer in the syringe according to the corresponding number, a buffer Corresponding to two sampling vials, a total of 8 sampling vials were loaded. The buffer in the sample bottle ...

Embodiment 3

[0064] Embodiment 3. Co-surfactant type investigation

[0065]3.1 Take three clean 30ml wide-mouth bottles, numbered 1, 2, and 3. Add 229 μL isopropanol to No. 1 bottle, add 221 μL pentanol to No. 2 bottle, and add 190 μL cyclohexanol to No. 3 bottle, respectively. Add 18 mg of 3-sulfopropyldodecyl dimethyl betaine, 16.6 μL of ethyl acetate, 750 μL of 20 mM borax aqueous solution, and 2.037 ml of water into three bottles, and ultrasonically (100W, 40kHz) at 30°C for 20 minutes to form a microemulsion buffer solution.

[0066] 3.2 Take three 0.22μm organic membrane filters, corresponding to No. 1-3 syringes numbered 1, 2, and 3, and use the filter to filter the buffer in the syringe according to the corresponding numbers. One buffer corresponds to two A sample vial was loaded into a total of 6 sample vials. The buffer in the sample bottle is flushed into the capillary column as the buffer for capillary electrophoresis, and the reference solution is injected.

[0067] Capilla...

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Abstract

The invention provides a fluorescence detection method of flavones in hawthorn fruits. The method comprises the following steps: extracting the hawthorn fruits by methanol to obtain a hawthorn fruit extract liquid, diluting to obtain a sample liquid, carrying out capillary electrophoresis fluorescence detection, and calculating according to the standard curve of a reference substance to obtain the content of each flavones in the hawthorn fruits, wherein a capillary column is filled with a micro-emulsified buffer solution, the components of the micro-emulsified buffer solution comprise 3-12mg / mL of a zwitterionic surfactant, 55-70mg / mL of a cosurfactant, 4.5-6mg / mL of ethyl acetate, 2.5-10mmol / L of a buffer salt, and the pH value of the micro-emulsified buffer solution is 8-10. The micro-emulsified buffer solution having the advantages of simple preparation, good dissolving ability, low viscosity and good stability is provided against the disadvantages of present chromatographic techniques, and the neutral zwitterionic surfactant is innovatively used in the buffer solution; and a light emitting diode-induced fluorescence detector having high sensitivity and good repeatability is preferentially used in the invention, so a substantial effect in the flavones detection is achieved.

Description

technical field [0001] The invention relates to a traditional Chinese medicine detection method using a light-emitting diode to induce a fluorescent device, which can realize the quality detection of different flavonoid components in hawthorn, and belongs to the technical field of traditional Chinese medicine analysis and detection. Background technique [0002] Hawthorn, the dry ripe fruit of Crataegus pinnatifida Bge.var.major N.E.Br. or Hawthorn Crataegus pinnatifida Bge. Hawthorn contains sugar, protein, flavonoids, carotene, starch, malic acid, calcium, iron and other substances, but the content of flavonoids is the highest. Hawthorn has been used as medicine for a long time. According to the Pharmacopoeia, hawthorn has the functions of eliminating accumulation and stagnation, promoting blood circulation and removing stasis, dilating blood vessels, increasing coronary blood flow, improving heart vitality, stimulating the central nervous system, softening blood vessels, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/76G01N1/28
Inventor 曹君曹婉胡帅帅庞潇卿
Owner HANGZHOU NORMAL UNIVERSITY
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