Precolumn derivatization and high-efficiency liquid-phase fluorescence detection method for tetrodotoxin and kit based on the method
A technology of tetrodotoxin and pre-column derivatization, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high detection cost and easy false positives in ELISA, and achieve low detection cost, high sensitivity, and avoid reagent errors.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1 The detection of tetrodotoxin in the sample to be tested combined with C18 solid phase extraction column and WCX solid phase extraction column purification
[0041] (1) Extraction and purification of the sample: Weigh 2 g of the homogenized puffer fish sample, add 10 mL of 0.1% acetic acid, ultrasonically extract in a water bath at 85°C for 10 min, then boil in a water bath for 5 min, and centrifuge at 4500 r / min after cooling For 5 min, take all the supernatant and pass it through a C18 solid-phase extraction column (activated with 3mL methanol and 3mL 0.1% acetic acid before use), and then wash with 2mL 0.1% acetic acid; collect all the filtrate and adjust to pH with ammonia water =7.0, all of them passed through a WCX solid-phase extraction column (activated with 3mL of 10% ammonia methanol and 1mL of water before use), washed with 2mL of water, drained the column, and eluted with 5mL of 2% acetic acid-methanol. The eluate was blown dry with nitrogen in a...
Embodiment 2
[0045] Example 2 The detection of tetrodotoxin in the sample to be tested combined with tetrodotoxin affinity chromatography column purification
[0046] (1) Extraction and purification of the sample: Weigh 2 g of the homogenized puffer fish viscera sample, add 10 mL of 0.1% acetic acid, ultrasonically extract in a water bath at 85°C for 10 min, then boil in a water bath for 5 min, and centrifuge at 4500 r / min for 5 min after cooling. min, take all the supernatant, adjust the pH to 7.4 with 0.5mol / L disodium hydrogen phosphate solution, pass through the tetrodotoxin affinity chromatography column (3mL specification) at a speed of 2mL / min, and wash with 3mL PBS (0.01mol / L, pH=7.4), washed with 3mL deionized water, eluted with 5mL 1% acetic acid, and drained the column. The eluate was blown dry with nitrogen in a water bath at 90°C and dissolved in 1 mL of water to obtain a sample solution.
[0047] (2) Fluorescence derivatization: Take 0.4mL of the sample solution, add 0.2m...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com