A joint method of remediation of petroleum-contaminated soil by Alkaline grass and microbiome

A technology for oil pollution and joint restoration, applied in the field of environmental pollution restoration, can solve the problems of secondary pollution, unsuitable for large-scale application, high cost, and achieve the effects of no secondary pollution, small amount of engineering, and reduced water evaporation.

Active Publication Date: 2015-11-04
TIANJIN UNIV OF SCI & TECH
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Problems solved by technology

Traditional physical and chemical methods are not suitable for large-scale application due to high cost and easy to produce secondary pollution

Method used

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  • A joint method of remediation of petroleum-contaminated soil by Alkaline grass and microbiome
  • A joint method of remediation of petroleum-contaminated soil by Alkaline grass and microbiome
  • A joint method of remediation of petroleum-contaminated soil by Alkaline grass and microbiome

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Experimental program
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preparation example Construction

[0026] The preparation method of the oil-degrading bacterial agent is as follows: transfer the two strains stored in the slant culture medium to 100mL beef extract peptone medium for activation and culture, and inoculate the activated strains into the fermentation medium at a ratio of 1%. Expand the culture in the tank, sterilize the wheat bran in a sterilization box with high-pressure steam, after cooling, mix the obtained fermentation liquid and wheat bran at a ratio of V (mL): W (g) at 1:1, air dry naturally, and pack into bags Sealed to make biological solid bacterial agent.

[0027] Activation medium composition: 0.5% beef extract, 1% peptone, 0.5% NaCl, pH 7.0-7.2, steam sterilization at 121°C for 20 minutes.

[0028] Composition of fermentation medium: hydrolyzed sugar 2.5%, corn steep liquor 2.5%, dipotassium hydrogen phosphate 0.15%, magnesium sulfate 0.04%, urea 0.4%, pH 6.8-7.0.

[0029] The soil used for planting S. chinensis is polluted soil with a mass fraction ...

Embodiment 1

[0032] The experiment site is located in Tianjin University of Science and Technology’s Estuary and Coastal Zone Environment and Ecology Research Laboratory. The soil for the potting test is a combination of oil-contaminated soil and clean soil, that is, the oil-contaminated soil collected near the oil storage tank of CNOOC Environmental Technology Service Branch. (0-20cm) and the natural pollution-free soil (0-20cm) collected in the flower pond of Tianjin University of Science and Technology TEDA Campus.

[0033] Plant tolerance test to petroleum hydrocarbons: a total of five soil controls were set up, namely CK (clean soil), petroleum hydrocarbon contaminated soil soil A (5000mg / kg), soil B (10000mg / kg), soil C (20000mg / kg ) Soil D (30000mg / kg).

[0034] Contaminated soils with total petroleum hydrocarbon mass fractions of 5000mg / kg, 10000mg / kg, 20000mg / kg and 30000mg / kg were prepared by weight ratio. Add 200g of soil samples to each petri dish. After hydroponic expansion a...

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Abstract

The invention relates to a method for repairing petroleum-contaminated soil through the combination of alkali grass and microbes. The method includes the steps of evenly mixing a petroleum degrading microbial agent with the soil according to the weight ratio of 1:5, scattering alkali grass seeds into the soil, conducting watering, waiting for plant sprouting, and conducting management according to the requirements of normal growth of plants, wherein the total mass fraction of petroleum hydrocarbon in the soil used for alkali grass plantation is 10000+5000mg / kg. According to the method, the microbial agent is added to the oil-contaminated saline alkali soil; the microbial agent and the petroleum-contaminated saline alkali soil are evenly mixed; then, the alkali grass seeds are sown; watering is regularly conducted; and the plants and microorganisms cooperate to degrade the petroleum contaminant in the soil. The alkali grass has the effect of promoting degrading of petroleum hydrocarbon and has the greening effect; both the degradation rate and the degradation speed are increased by adding the microbial agents; organic matter obtained after microorganism degradation can serve as a vegetable fertilizer; the plants and microorganisms benefit each other and grow together; the degrading time is long; and therefore a good removal effect is achieved for contaminants such as petroleum hydrocarbon.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution remediation, and relates to a method for jointly remediating oil-polluted soil by Alkalima and microbiome. Background technique [0002] Petroleum is one of the main energy sources in modern society, known as "industrial blood". Now there are more than 150 oil-producing countries and regions, and more than 40,000 oil and gas fields have been discovered. Among them, my country has found more than 400 oil-producing countries with an annual output of nearly 200 million tons. It is one of the top ten oil-producing countries in the world. However, in the process of oil extraction, smelting and transportation, sudden accidents, leakage or improper operation will cause a lot of pollution to the surrounding soil. After oil enters the soil, it will be adsorbed on the surface of soil particles, block soil pores, reduce soil water permeability, change soil organic matter composition, struct...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/00B09C1/10
Inventor 刘宪斌李厦田胜艳赵兴贵
Owner TIANJIN UNIV OF SCI & TECH
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