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Dual-fluorescence functional polymer nanometer microsphere with pH response and application thereof in tumor tissue detection

A technology of nano-microspheres and polymers, which is applied in the field of fluorescent materials, can solve problems such as interference and easy errors in detection results, and achieve the effect of good response function

Active Publication Date: 2014-08-27
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the detection of traditional fluorescent markers, imaging materials with a single fluorescent color are mainly used, and the background fluorescent signal in the biological environment interferes with it to a large extent, resulting in errors in the detection effect.

Method used

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  • Dual-fluorescence functional polymer nanometer microsphere with pH response and application thereof in tumor tissue detection
  • Dual-fluorescence functional polymer nanometer microsphere with pH response and application thereof in tumor tissue detection
  • Dual-fluorescence functional polymer nanometer microsphere with pH response and application thereof in tumor tissue detection

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024](1) Weigh 4.42mmol of NIPAM monomer and dissolve it in 185mL deionized water, add it to a 500mL three-necked bottle, add 1.4×10 -6 mol of rare earth complex Eu(TTA) 3 Phen, 5mL of styrene (St), at room temperature and under nitrogen protection, mechanically stirred (400rpm) for 30min, then gradually warmed up to 70°C, added 15mL of an aqueous solution containing 0.3mmol of initiator potassium persulfate (KPS) in the system, and initiated polymerization. The reaction was carried out for 12h to end. The polymer seed microspheres containing the rare earth complex are obtained. Impurities such as unpolymerized monomers and initiators in the liquid phase were removed by high-speed centrifugation (18500 r / min), and then dissolved in 100 mL of deionized water for use.

[0025] (2) Slowly pour the seed microsphere solution obtained in step (1) into a 500mL three-neck bottle, use this as the seed, synthesize EuPS / PNIPAM-PAA core-shell microspheres by seed emulsion polymerizati...

Embodiment 2

[0029] (1) Weigh 5 mmol of NIPAM monomer and dissolve it in 100 mL of deionized water, add it to a 500 mL three-necked bottle, add 1.5×10 -6 mol of CdTe, 5mL of fluorostyrene (F-St), under the protection of nitrogen at room temperature, mechanical stirring (400rpm) for 30min, then gradually warming up to 70°C, adding 10mL of sodium persulfate (Na 2 S 2 o 8 ) 0.3mmol aqueous solution in the system to initiate polymerization, and the reaction was carried out for 12h to end. The obtained CdTe-containing polymer seed microspheres were centrifuged at a high speed (18500r / min) to remove impurities such as unpolymerized monomers and initiators in the liquid phase, and then dissolved in 100 mL of deionized water for use.

[0030] (2) Slowly pour the seed microsphere emulsion obtained in step (1) into a 500mL three-necked bottle as a seed, synthesize CdTe-PFS / PNIPAM-PMAA core-shell microspheres by seed emulsion polymerization, and add 45mmol NIPAM, 3.5mmol MAA, at room temperature a...

Embodiment 3

[0034] (1) Weigh 5 mmol of NIPAM monomer and dissolve it in 190 mL of deionized water, add it to a 500 mL three-necked bottle, add 2×10 -6 mol of RhB, 5mL of styrene (St), at room temperature and under the protection of nitrogen, stir mechanically (400rpm) for 30min, then gradually raise the temperature to 70°C, add 10mL of an aqueous solution containing 0.3mmol of initiator ammonium persulfate (APS) to the system , Initiate polymerization, and the reaction ends after 12h. The obtained RhB-containing polymer seed microspheres. Impurities such as unpolymerized monomers and initiators in the liquid phase were removed by high-speed centrifugation (18500 r / min), and then dissolved in 100 mL of deionized water for use.

[0035] (2) Slowly pour the seed microsphere solution obtained in step (1) into a 500mL three-necked bottle, and use this as a seed to synthesize RhB-PS / PNIPAM-PMAA core-shell microspheres by seed emulsion polymerization, and inject them into the system Add 40mmol...

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Abstract

The invention discloses a dual-fluorescence functional polymer nanometer microsphere with pH response and application thereof in tumor tissue detection and belongs to the technical field of high polymer materials. The method comprises the following steps: firstly synthesizing an emulsion microsphere of a composite fluorescence molecule A, introducing a polymer with a pH response function into a shell layer of the microsphere by adopting a seed emulsion polymerization method, and thus preparing the emulsion microsphere with a core-shell structure; further compounding a fluorescence molecule B into the shell layer of the microsphere, and thus obtaining the dual-fluorescence-emission functional polymer nanometer microsphere with pH response. The microsphere presents different fluorescence intensity and fluorescence color under different pH value conditions from acidity to alkalinity. The microsphere is applied to tissue detection based on the pH response function of the nanometer microsphere fluorescent property, the microsphere presents violet fluorescence in the normal tissue (pH about 7.4), the microsphere presents pink fluorescence in the tumor tissue (pH of less than 6.5), and the microsphere can be used for preparing a fluorescent material and has wide application prospects in the fields of tumor cell and tissue detection, drug sustained release and the like.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a pH-responsive double fluorescent functional polymer nanometer microsphere and a fluorescent material used in the preparation of tumor tissue detection. Background technique [0002] Smart materials are new materials that can sense, respond to the environment, and have the ability to discover functions. There are mainly temperature sensitive, pH sensitive, electrical sensitive, light, magnetic and biosensitive hydrogel materials. Although the history of research and development of intelligent materials is not long, due to their unique properties, they are not only widely used in industrial and agricultural production and daily life, but also play an important role in the development of high-tech. In recent years, the research work on smart materials has been unprecedentedly active, among which new polymers with smart behavior and their hydrogels are develo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F212/08C08F212/14C08F220/54C08F220/06C08F220/14C08F2/44C08F2/22C09K11/02C09K11/06C09K11/88G01N33/52
Inventor 林权杨旭东陈洁陈阳孙源卿杨雪杨柏董凤霞
Owner JILIN UNIV
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