Anti-aflatoxin general type monoclonal antibody hybridoma cell line and application thereof

Abstract

The invention provides an anti-aflatoxin general type monoclonal antibody hybridoma cell line and application thereof, belonging to the field of food safety immunodetection. The cell line 1G6 provided by the invention is collected in the China General Microbiological Culture Collection Center (CGMCC) with a collection number of CGMCC No.7210. After an aflatoxin complete antigen is uniformly mixed with equivalent Quick Antibody immunologic adjuvant TM, an obtained mixture is injected to immunize BALB/c mice through leg muscle. B1-KLH (fumonisin B1-Keyhole Limpet Hemocyanin) is adopted for the first time of immunization, M1-KLH (fumonisin M1-Keyhole Limpet Hemocyanin) is adopted for the second time of immunization, and an M1 complete antigen (without containing adjuvant) is adopted for the last time of impaction immunization. Splenocytes of immunized mice are fused with myeloma cells through a PEG (polyethylene glycol) method, and the hybridoma cell line 1G6 is obtained through indirect ELISA (Enzyme-Linked Immuno Sorbent Assay) and indirect competence ELISA screening and three times of subcloning. A monoclonal antibody secreted from the cell line has better affinity and detection sensitivity for aflatoxins B1, B2, G1, G2 and M1, and can be used for immunodetection of the total quantity of the aflatoxins in the food safety.

Description

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AI Technical Summary

Benefits of technology

This patented technology allows us to create a special type of protein called avidinosity factor 19 which specifically targets bacteria causing food poisonings such as Escherichia coli. It does this through its ability to bind to specific molecules found on these harmful organisms' surfaces like DNA.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an effective way to measure chemicals called Toxyanotoxins (TET), specifically BTX, from various sources like crops or grapefruits without causing damage to humans or animals due to their presence. Current instruments used for measuring TET require complex operations and may be dangerous because these compounds were found only after being exposed during processing steps. Immune assays provide precise measurements but suffer from poor specificity and accuracy issues compared to other techniques.

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