Method for performing cross breeding on water chestnut and Dongkui waxberry for waxberry cultivating variety

Abstract

The invention discloses a method for performing cross breeding on water chestnut and Dongkui waxberry for waxberry cultivating variety. The method comprises the following steps: by taking water chestnut as a female parent and taking male flower of Dongkui waxberry as a male parent, pollinating during water chestnut full-blossom period; collecting seeds after the fruits are ripened, storing the seeds at the temperature of 5 DEG C, seeding to obtain F1 plants; and screening true hybrid plants from the F1 plants. Intervarietal cross breeding between the waxberry cultivating varieties such as water chestnut and Dongkui waxberry is realized for the first time, true hybrid plants with certain genotypes are obtained, and a novel technological approach is provided for breeding novel waxberry variety. Moreover, the filial generation is subjected to hybrid identification by amplifying specific expression SSR markers of the F1 generation, the identification process is simple and rapid and the cost is low, the identification result is stable and reliable, and the gap of the molecular biological identification technology of waxberry intervarietal hybridization is filled.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for hybrid breeding of bayberry cultivars water chestnut and dongkui. Background technique [0002] Red bayberry (Myrica rubra Sieb. & Zucc.) is a plant of the genus Myrica rubra in the Myricaceae family, and is a characteristic fruit in southern my country. In 2013, the national waxberry cultivation area reached 300,000 hectares, with an output of 1.2 million tons and an output value of 10 billion yuan. It is mainly distributed in Zhejiang, Jiangsu, Fujian, Jiangxi, Hunan, Guangdong, Guizhou and Yunnan provinces, of which Zhejiang has the largest area and output. , reaching 80,000 hectares and 388,000 tons respectively. Red bayberry fruit is rich in anthocyanins, which has high nutritional value and has been widely concerned. In addition, as one of the "pioneer tree species" for afforestation in mountainous areas, bayberry has the functions of maintaining wate...

AI Technical Summary

Problems solved by technology

However, the hybridization between cultivars has not been reported at present, and its main reason is that it is difficult to realize the interspecific hybridization of cultivars because the cultivars of Myrica rubra are all female plants.

If ordinary male plants are used for hybridization, the fruit varieties of hybrid offspring cannot be predicted, and there will be a large proportion of male plant offspring, and the breeding efficiency is low

[0006] For how to distinguish F 1 As a hybrid, it mainly relied on various morphological markers to classify bayberry in the early stage. However, as a perennial plant, bayberry has a highly complex genotype, and the sexual time is too long. It takes too long to screen and classify through morphological markers

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