Unlock instant, AI-driven research and patent intelligence for your innovation.

Compound used as Wnt signal pathway activator, and preparation and application thereof

A technology of compounds and signals, applied in medical preparations containing active ingredients, anti-inflammatory agents, drug combinations, etc., can solve the problem that there is no obvious progress in research.

Active Publication Date: 2014-09-10
CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI +1
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since the Wnt / β-catenin signaling pathway is related to many cancers and diseases, the out-of-control Wnt / β-catenin signaling pathway can be used as a good way to treat diseases related to the Wnt / β-catenin signaling pathway, but at this stage There has been no significant progress in the research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound used as Wnt signal pathway activator, and preparation and application thereof
  • Compound used as Wnt signal pathway activator, and preparation and application thereof
  • Compound used as Wnt signal pathway activator, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] 1. Experimental materials

[0083] 1) Plasmid

[0084] TOPFlash plasmid was purchased from Millipore Corporation.

[0085] GFP plasmid was purchased from Millipore Corporation.

[0086] 2) Antibody

[0087] The antibody of β-actin (sc-47778) was purchased from Santa Cruz; the antibody of SP1 (S9809) was purchased from Sigma; the antibody of β-catenin (610154) was purchased from BD Biosci.Pharmingen.

[0088] 3) Reagents

[0089] Protease inhibitors (complete protease inhibitor mixed tablet) and NP-40 were purchased from Roche; Lipofectamine and PLUS transfection reagent were purchased from Invitrogen;

[0090] Wnt3a conditioned medium was prepared from Wnt3a cell line (CRL-2647, purchased from ATCC cell bank in the United States): Wnt3a cells were cultured in DMEM cell medium supplemented with 10% calf serum for 4 days, and then the supernatant medium was collected by centrifugation.

[0091] The control conditioned medium was prepared from the L cell line (CRL-2648,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides compound used as a Wnt signal pathway activator, and a preparation and an application thereof. The compound is a compound of formula (I) or formula (II). The compound can be used for preparing medicines for adjusting a classic Wnt signal pathway, and can also be used for researching the classic Wnt signal pathway. The compound also provides a new idea for screening the medicines for adjusting the Wnt signal pathway and medicines for preventing and treating classic Wnt signal pathway related diseases, and researching the application of the Wnt signal pathway in stem cells.

Description

technical field [0001] The present invention relates to a compound that can be used as an activator of Wnt signaling pathway and its preparation and application. Background technique [0002] The Wnt signal transduction pathway is a highly conserved signal transduction pathway in the evolution of organisms, regulating and controlling many life activities. In the early development of animals, Wnt signaling determines a series of important events such as the formation of the dorsal-ventral axis, germ layer establishment, somite differentiation, tissue or organ formation, and directly controls proliferation, differentiation, polarization, apoptosis and anti-apoptosis [Clevers, H. and R. Nusse, Wnt / beta-Catenin Signaling and Disease. Cell, 2012.149(6): p.1192-205]. More than a dozen members of Wnt proteins are involved in different signal transduction pathways by interacting with different receptors on the cell membrane. These pathways are mainly divided into the canonical Wnt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07D491/056C07D519/00A61K31/4741A61K31/473A61P25/28A61P19/02A61P29/00A61P19/10A61P7/00
CPCA61K31/4741C07D491/056C07D519/00
Inventor 李林汪胜郝小江潘巍峻尹俊林聂芬陈铎之
Owner CENT FOR EXCELLENCE IN MOLECULAR CELL SCI CHINESE ACAD OF SCI