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Method for manufacturing blood stagnation erectile dysfunction animal model

A technology for erectile dysfunction and its production method, which is applied in the fields of medicine and animal behavior, can solve problems such as model instability and easy reversal, and achieve the effect of convenient implementation, easy quantification, and strengthening the effect of blood stasis

Inactive Publication Date: 2014-09-24
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing models of traumatic blood stasis, heat-toxin blood stasis, blood deficiency and blood stasis mostly use acute modeling stimuli, which are inconsistent with the chronic pathogenesis of ED; The stimulation model has the shortcomings of only local stimulation, the model only changes biochemical indicators and cannot produce the biological signs of blood stasis syndrome in animals, the model is unstable, and easy to reverse [Yan Linlin, Zhou Lihong, Li Qi. Research overview of animal models of blood stasis syndrome in traditional Chinese medicine. Traditional Chinese Medicine Journal, 2014,55(3):255-258.]

Method used

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  • Method for manufacturing blood stagnation erectile dysfunction animal model
  • Method for manufacturing blood stagnation erectile dysfunction animal model
  • Method for manufacturing blood stagnation erectile dysfunction animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Electric shock: the rats were placed in the electric shock equipment covered with wire mesh, and given continuous electric stimulation (current 1mA) for 80s, repeated at intervals of 15min, for a total of 8 stimulations. Do it every morning and afternoon.

[0028] (2) Noise: the rats were placed in a closed noise device, given high-frequency noise stimulation (100dB, 10000Hz) for 80s, repeated every 15min, and stimulated 8 times in total. Do it every morning and afternoon.

[0029] (3) Ice swimming: subcutaneously inject 0.1% epinephrine hydrochloride 0.08ml / kg before stimulation, and after an interval of 1h, put the rats into a swimming bucket mixed with ice and water, add water to the swimming bucket to about 2 / 3 height of the bucket body, and Add about 400 g of crushed ice (keep temperature at 0°C). Perform ice swimming for 5 minutes, and quickly dry it with a dry towel after the end of the ice swimming. One hour after ice swimming, another epinephrine injectio...

Embodiment 2

[0031] (1) Electric shock: the rats were placed in the electric shock equipment covered with wire mesh, and given continuous electric stimulation (current 0.8mA) for 100s, repeated at intervals of 10min, for a total of 6 stimulations. Do it every morning and afternoon.

[0032](2) Noise: the rats were placed in a closed noise device, and given high-frequency noise stimulation (100dB, 10000Hz) for 160s, repeated at intervals of 10min, a total of 6 stimulations. Do it every morning and afternoon.

[0033] (3) Ice swimming: subcutaneously inject 0.1% epinephrine hydrochloride 0.05ml / kg before stimulation, and after an interval of 0.5h, put the rats into a swimming bucket mixed with ice water, add water to the swimming bucket to about 2 / 3 height of the bucket body, And add about 400g crushed ice. Perform ice swimming for 10 minutes, and quickly dry it with a dry towel after the end of the ice swimming. After 0.5 hours of ice swimming, give another injection of epinephrine, the d...

Embodiment 3

[0035] (1) Electric shock: the rats were placed in the electric shock equipment covered with wire mesh, and given continuous electric stimulation (current 1.2mA) for 60s, repeated every 20min, for a total of 10 stimulations. Do it every morning and afternoon.

[0036] (2) Noise: the rats were placed in a closed noise device, and given high-frequency noise stimulation (150dB, 20000Hz) for 80s, repeated every 20min, for a total of 10 stimulations. Do it every morning and afternoon.

[0037] (3) Ice swimming: subcutaneously inject 0.1% epinephrine hydrochloride 0.1ml / kg before stimulation, and after an interval of 1.5h, put the rats into a swimming bucket mixed with ice water, add water to the swimming bucket to about 2 / 3 height of the bucket body, And add about 400g crushed ice. Perform ice swimming for 10 minutes, and quickly dry it with a dry towel after the end. 1.5 hours after ice swimming, give another injection of epinephrine, the dose is the same as before. Do it every...

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Abstract

The invention relates to a method for manufacturing a blood stagnation erectile dysfunction animal model. The method comprises the following steps that S1, a rat is placed in electric shock equipment and is given with continuous electrophotoluminescence; S2, the rat is placed in noise equipment and given with high-frequency noise stimulation; S3, the rat is placed in swimming equipment with ice and water mixed and made to swim. The blood stagnation ED rat model is successfully built by adopting composite factors of electric shocks, noise and ice swimming, the situations that the stimulation strength is weak, model forming time is prolonged, and model forming efficiency is low are avoided, and meanwhile the problem that adaptation easily occurs due to single weak stimulated animals is solved. In addition, the composite factors of the method are convenient to implement, quantification is easy, currents and the noise can be controlled within a proper range, and the model forming efficiency is high.

Description

technical field [0001] The invention relates to the fields of medicine and animal behavior, and relates to a method for establishing an animal model of erectile dysfunction of blood stasis syndrome. Background technique [0002] Erectile dysfunction (erectile dysfunction, ED, known as "impotence" in traditional Chinese medicine) is a common male disease and has always been a research hotspot in urology and andrology. In the theory of etiology and pathogenesis of traditional Chinese medicine, it is believed that stagnation of liver Qi stagnates due to emotional stimulation affecting the dredging function of the liver, or due to sexual indulgence, sexual labor, chronic illness and physical weakness, the deficiency of essence and blood, and the consumption of kidney qi, resulting in stagnation of blood stasis. Blood stasis is the important pathological factor of impotence [Li Liuji, Yan Jilan. Brief introduction to the etiology and pathogenesis of impotence. Jilin Traditional...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B19/00
Inventor 戴继灿陈翔胡超崔杰峰
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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