Anti-swine fever virus infection siRNA (small interfering ribonucleic acid) and application thereof

A technology of swine fever virus and adenovirus, which is applied in the direction of DNA / RNA fragments, application, recombinant DNA technology, etc., can solve the problem that there is no anti-swine fever virus siRNA, etc., and achieve the effect of strong interference or inhibition activity and effective prevention and control

Inactive Publication Date: 2014-09-24
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The exact function of NS5A in the CSFV life cycle is poorly understood, and no anti-CSFV siRNAs targeting CSFV P7 and NS5A genes have been reported so far

Method used

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  • Anti-swine fever virus infection siRNA (small interfering ribonucleic acid) and application thereof
  • Anti-swine fever virus infection siRNA (small interfering ribonucleic acid) and application thereof
  • Anti-swine fever virus infection siRNA (small interfering ribonucleic acid) and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Design and Screening of Anti-Swine Fever Virus siRNA

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PUM

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Abstract

The invention discloses an anti-swine fever virus infection siRNA (small interfering ribonucleic acid) and application thereof, belonging to the field of identification and application of anti-swine fever virus infection siRNA. The invention firstly discloses an anti-swine fever virus infection siRNA for Npro, P7, NS5A and NS5B genes in targeted swine fever virus genome, and the nucleotide sequences are respectively disclosed as SEQ ID NO.1-12. The constructed recombinant swine fever virus is further utilized to screen out the siRNA with the highest interference or inhibition activity for swine fever virus, and the nucleotide sequence is disclosed as SEQ ID NO.1, SEQ ID NO.7 or SEQ ID NO.12. The invention also discloses an expression vector containing the anti-swine fever virus infection siRNA and application thereof. The anti-swine fever virus infection siRNA has high inhibition activity for swine fever virus, and has the potential to be prepared into drugs or reagents for preventing or treating swine fever.

Description

technical field [0001] The present invention relates to anti-CSF virus siRNA, especially relates to the anti-CSF virus infection siRNA targeting CSF virus genome, the present invention further relates to the anti-CSF virus infection siRNA targeting CSF virus genome in preparation prevention or The application of the medicine or reagent for treating swine fever belongs to the field of identification and application of anti-swine fever virus siRNA. Background technique [0002] Classical swine fever (CSF) is a contact and fatal infectious disease caused by classical swine fever virus (CSFV), characterized by high fever and hemorrhage, and extremely high morbidity and mortality , is listed as an animal disease that must be declared by the World Organization for Animal Health (OIE). [0003] Classical swine fever virus (CSFV) is a member of the genus Pestivirus in the Flaviviridae family and is a single-stranded positive-strand linear RNA molecule. The CSFV genome is 12.3kb in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113A61K48/00A61P35/00C12N15/861C12N15/867C12N15/85
CPCC12N15/1131C12N2310/14
Inventor 仇华吉申梁李永锋孙元李素罗玉子
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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