Method for evaluating cleaning effect of food production equipment
A technology for producing equipment and cleaning effects, which is applied in the direction of analysis, measuring devices, instruments, etc. through chemical reactions of materials, which can solve problems that cannot reflect the cleaning effect of equipment, microorganisms do not have enough growth and reproduction time, and cannot detect CIP in time, etc. question
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Embodiment 1
[0053] Utilize the evaluation method provided by the present invention to evaluate the cleaning effect of the milking equipment (sampling points include metering bottle, breast milker, milk liner, milk suction tube, filter tube, milk tank, etc.) after cleaning and disinfection for 5 hours. The traditional microbial smear method is verified, and the specific steps are as follows:
[0054] Inorganic substance residue detection: After rinsing with deionized water on the surface of the designated part, wipe the sample with a cleaning verification cotton swab dipped in dilute nitric acid (concentration: 0.1mol / L), and the sampling area is 20-30cm 2 ;After sampling, put the cotton swab into a small test tube, add 3mL inorganic substance residue detection reagent, react at room temperature for 3-5 minutes, compare the reaction solution with the inorganic substance residue color card to determine the residue level;
[0055] Organic matter residue detection: on the surface of the desig...
Embodiment 2
[0064] The detection and analysis results for milking equipment in embodiment 1 are respectively as follows Figure 3a-Figure 3f As shown, among them, Figure 3a It is the detection and analysis result at the lower mouth of the metering bottle, Figure 3b It is the detection and analysis result at the lower plate of the breast milker, Figure 3c It is the detection and analysis result of the inner wall of the milk liner, Figure 3d It is the detection and analysis result at the lower mouth of the breast pump, Figure 3e is the detection and analysis result of the inner wall of the milk tank, Figure 3f It is the detection and analysis result at the lower port of the filter tube. From Figure 3a-Figure 3f According to the results shown, except for the lower mouth of the breast pump, the growth of microorganisms on the surface of the other equipment has a certain correlation with the level of organic matter residues, that is, when the level of organic matter residues is 3 o...
Embodiment 3
[0066] Stability test of organic residue detection reagent:
[0067] According to the calculation of 80% protein content in WPC80, accurately weigh 0.625g WPC80, prepare 100mL standard solution I with a concentration of 5mg / mL with distilled water; take 10mL standard solution I, prepare 100mL standard solution I with a concentration of 500μg / mL with distilled water Solution II; dilute 20mL of standard solution I to 100mL with distilled water to make working solution III with a concentration of 1mg / mL; use working solution II, III and distilled water to prepare protein content of 10, 30, 60, 90, 120 , 150, 210, and 240 μg of the solution (see Table 6 for the volume of the required standard solution), add distilled water to 0.3 mL, add 1 mL of organic matter residue detection reagent A and 20 μL organic matter residue detection reagent B, and measure the absorbance after incubation at 60 ° C for 10 min. And measure the absorbance after incubating at 60° C. for 10 minutes and the...
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