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Preparation technology for enzymolysis bioactive peptide nanometer selenium

A preparation process and nano-selenium technology are applied in the field of preparation technology of enzymatically hydrolyzed active peptide nano-selenium, which can solve the problems of single function of protein powder, poor taste of protein powder, difficult operation, etc., achieve low process requirements, improve body Immunity, easy to digest and absorb

Inactive Publication Date: 2015-06-10
HUZHOU WUFU AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the step-by-step enzymatic hydrolysis, the process is complicated, the operation is difficult, and the cost is high. The protein powder produced has a poor taste and can only be used as feed, and does not maximize the utilization of the protein in the intestinal mucosa.
And the function of the obtained protein powder is single

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A preparation process of enzymolysis active peptide nano-selenium, the steps are as follows: (1) in enzymolysis irrigation, the powder of the by-product after extracting heparin from the pig intestinal mucosa is dissolved in water 5 times its mass to obtain a solution, and the temperature is raised to 65 ℃, add the compound enzyme, adjust the pH to 7.0 with baking soda, enzymolyze the pig intestinal mucosa for 6 hours, then raise the enzymatic hydrolysis temperature to 90°C to inactivate the enzyme for half an hour, obtain the enzymatic hydrolysis solution, and keep it at 60°C; the compound enzyme is A mixture of papain, neutral protease and flavor enzyme in a mass ratio of 100:50:10, the mass ratio of its total mass to the lysate is 6:100; (2) carboxymethyl chitosan, selenous Sodium bicarbonate and vitamin C were mixed according to the mass ratio of 80:1:10, and the nano-selenium solution was prepared in the water phase, the temperature was controlled at 60 ° C, and the...

Embodiment 2

[0025] A preparation process of enzymolysis active peptide nano-selenium, the steps are as follows: (1) in enzymolysis irrigation, the powder of the by-product after extracting heparin from the pig intestinal mucosa is dissolved in water six times its mass to obtain a solution, and the temperature is raised to 60 ℃, add the compound enzyme, adjust the pH to 7.8 with baking soda, and enzymolyze the pig intestinal mucosa for 6 hours, then raise the enzymatic hydrolysis temperature to 90°C to inactivate the enzyme for half an hour, and obtain the enzymatic hydrolysis solution, which is kept at 55°C; the compound enzyme is A mixture of papain, neutral protease and flavor enzyme in a mass ratio of 150:100:10, the mass ratio of its total mass to the lysate is 7:100; (2) carboxymethyl chitosan, selenous Sodium bicarbonate and vitamin C were mixed according to the mass ratio of 100:1:15, and the nano-selenium solution was prepared in the water phase, the temperature was controlled at 6...

Embodiment 3

[0027] A preparation process for enzymolysis of active peptide nano-selenium, the steps are as follows: (1) In enzymolysis irrigation, the powder of the by-product after extracting heparin from the pig intestinal mucosa is dissolved in water 5 times its mass to obtain a solution, and the temperature is raised to 60 ℃, add the compound enzyme, adjust the pH to 6.5 with baking soda, enzymolyze the pig intestinal mucosa for 6 hours, then raise the enzymatic hydrolysis temperature to 95°C to inactivate the enzyme for 20 minutes, obtain the enzymatic hydrolysis solution, and keep it at 55°C; the compound enzyme is A mixture of papain, neutral protease and flavor enzyme in a mass ratio of 120:80:10, the mass ratio of its total mass to the lysate is 5:100; (2) carboxymethyl chitosan, selenous Sodium bicarbonate and vitamin C are mixed according to the mass ratio of 90:1:12, and the nano-selenium solution is prepared in the water phase, and the temperature is controlled at 65 ° C for 2...

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Abstract

The invention relates to a preparation technology for enzymolysis bioactive peptide nanometer selenium. The preparation technology comprises the following steps: (1) a by-product which is formed by extracting heparins from animal intestine mucosas is in enzymolysis, enzymes are deactivated, enzymolysis liquid is obtained, and the mass ratio of added composite enzymes to chitlins mucosa liquid is 5-7:100; (2) chitosan, selenium compounds and vitamins C or citric acids are mixed in a definite proportion, and a nanometer selenium solution is prepared in an aqueous phase; (3) the nanometer selenium solution is added into enzymolysis liquid of which the temperature is 55-65 DEG C, to obtain mixed liquid, and the mixed liquid is heated to 45-60 DEG C to be reacted for 1-2 hours to obtain finished liquid; and (4) the finished liquid is concentrated and dried to obtain the enzymolysis bioactive peptide nanometer selenium. The preparation technology disclosed by the invention makes full use of protein resources, and at the same time, pollution to environment is also avoided. At the same time, the chitosan is used as a medium to absorb the nanometer selenium and polypeptide, so that multifunctional bioactive peptide albumen powder which is rich in the nanometer selenium, short peptide chains and balanced amino acids is formed, the nutritive value is high, the bioactive peptide nanometer selenium is easy to absorb, and various diseases can also be prevented.

Description

technical field [0001] The invention relates to a method for producing protein powder, in particular to a preparation process for enzymatically hydrolyzing active peptide nano-selenium. Background technique [0002] Intestinal mucosa is rich in protein resources, but it has not been fully developed and utilized. Domestic intestinal mucosa is mainly used to extract heparin, which is then treated as waste, causing pollution and waste. The Chinese patent No. CN101209082 disclosed a preparation method of functional feed protein intestinal membrane protein powder on August 31, 2011, using pig intestinal mucosa or its leftovers after extracting heparin as raw materials, using neutral protease, After step-by-step enzymolysis with papain, adding carriers such as soybean hulls or wheat bran and finally drying to obtain the protein powder. The method can effectively utilize discarded intestinal mucosa, reduce pollution and waste, and also solve the current situation of shortage of pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/305A23L1/304A23L1/30C12P21/06
CPCA23L33/16A23L33/17A23V2002/00C12P21/06A23V2200/30A23V2250/1626A23V2250/542
Inventor 陆杰坤
Owner HUZHOU WUFU AGRI TECH