A kind of nucleic acid aptamer of glycosylated hemoglobin and its preparation method

A technology of glycosylated hemoglobin and nucleic acid aptamer, applied in the field of protein detection, can solve the problems such as the screening preparation method has not been reported yet, the lack of research on the efficient and specific identification of glycosylated hemoglobin GHb, etc. Effects of Specificity and Sensitivity
CN104109675BActive Publication Date: 2016-07-06广西安仁欣生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广西安仁欣生物科技有限公司
Publication Date
2016-07-06

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Abstract

The invention discloses a nucleic acid aptamer of glycosylated hemoglobin GHb with high specificity and high affinity and a preparation method thereof. The nucleotide sequence of the glycosylated hemoglobin GHb nucleic acid aptamer includes any one of SEQ ID NO: 1 to SEQ ID NO: 4. The nucleic acid aptamer preparation method of glycosylated hemoglobin GHb includes: construction of random sequence oligonucleotide library, preparation of glycosylated hemoglobin-micromagnetic bead complex, initial screening of target substance-nucleic acid complex, and preparation of secondary single-stranded DNA oligonucleotide library , Screening and identification of glycosylated hemoglobin nucleic acid aptamers. The prepared nucleic acid aptamer is non-toxic, has a small molecular weight, is easy to synthesize and label, only specifically recognizes glycosylated hemoglobin GHb, does not recognize and bind to other homologous non-glycosylated hemoglobin Hb, and can become a molecular beacon for detecting the content of glycosylated hemoglobin GHb.
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Description

[0001] This application is a divisional application of "a nucleic acid aptamer for glycosylated hemoglobin and its preparation method". Nucleic acid aptamer and its preparation method". technical field

[0002] The invention belongs to the technical field of protein detection and relates to a nucleic acid, in particular to a nucleic acid aptamer of glycosylated hemoglobin GHb with high specificity and high affinity and a preparation method thereof. Background technique

[0003] Diabetes is a worldwide public health problem of human health. Diabetes is a lifelong disease, and its complications are the main cause of disability and death. Therefore, people hope that diabetes can be detected and treated as early as possible. Traditional diabetes diagnosis and treatment monitoring use fasting blood glucose, postprandial blood glucose and oral glucose tolerance test, etc., but blood glucose parameters only represent the instantaneous blood glucose level when blood is drawn, and gl...

Claims

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