Method for detecting concentration of synthetic peptide antigen

Abstract

A method for detecting concentration of a synthetic peptide antigen comprises the following steps: (1) preparing standard synthetic peptide solutions, namely diluting a synthetic peptide solution with a known concentration with distilled water to a concentration of 1 mg / mL, then continuing to dilute into different gradient concentrations; (2) preparing a synthetic peptide sample, namely employing distilled water to continuously dilute the synthetic peptide sample with an unknown concentration to different gradient concentrations; (3) putting an A reagent in a colorimetric ware, adding a to-be detected sample and mixing uniformly; (4) continuing to adding a B reagent, performing lucifugal reaction at room temperature for 20 min, and utilizing a spectrophotometer to measure the light absorption value at OD750 after the reaction is completely finished; and (5) according to the data corresponding to the gradient samples of the standard synthetic peptide solution samples to draft a standard curve, and performing conversion on the light absorption value of the synthetic peptide sample based on the standard curve, and calculating out the corresponding concentration value. Compared with the prior art, the method has the advantages of high sensitivity, small sample usage amount, wide detection scope, high pertinency and the like.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for detecting the concentration of a synthetic peptide antigen. Background technique [0002] Protein is a very important biomacromolecule, with many types, heterogeneous structure, great difference in molecular weight, and different functions, which brings many specific difficulties to the establishment of an ideal and general protein quantitative analysis method. . At present, there are many methods for determining the protein content of proteins, all of which are established according to the different properties of proteins, including: ultraviolet spectrophotometry, Kjeldahl method, biuret method, Folin phenol reagent method (Lowry method), bicinchoninic acid method (BCA method), colloidal gold method, Coomassie brilliant blue staining method, silver staining method, fluorescence method, etc. [0003] There are many methods for protein determination, but each method h...

AI Technical Summary

Problems solved by technology

For example, the Kjeldahl method is the most accurate, but the operation is complicated, and it is not qualified for the test of a large number of samples; the biuret method is simple to operate and has a good linear relationship, but the sensitivity is poor, the sample needs a large amount, and the measurement range is narrow. Therefore, the application in scientific research is limited; and the Folin phenol reagent method makes up for its shortcomings, so it is widely used in scientific research, but it has many interference factors; the Coomassie brilliant blue staining method was started because of its sensitivity and simplicity. It has received attention again; the BCA method is popular for its stable reagents, strong anti-interference ability, stable results, and high sensitivity; the colloidal gold method has high sensitivity, which can reach nanogram levels, and is used for the determination of trace proteins