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Judging method, judging device, judging system and program

A judging method and technology for detecting intensity, which is applied in measuring devices, image data processing, material inspection products, etc., and can solve problems such as changes in detection intensity and uneven selection of binding substances

Active Publication Date: 2016-08-17
TORAY IND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Here, although the selective binding amount of the test substance such as gene expression level is required to be reliable as data, for example, when the selective binding substance such as complementary DNA immobilized on the carrier as spots is not uniform and spots exist, There is a possibility that the detection intensity may change due to non-biological factors, such as when pollutants are attached to the spots.

Method used

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  • Judging method, judging device, judging system and program
  • Judging method, judging device, judging system and program
  • Judging method, judging device, judging system and program

Examples

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Embodiment 1

[0135] Hereinafter, Example 1 in which an appropriate threshold value was examined will be described. First, the same test substance was hybridized to two DNA chips, and it was confirmed that there was no large difference in detection intensity. Figure 11 This is a scattergram showing the detection intensity when the same test substance was hybridized to two DNA chips. The horizontal axis represents the detection intensity of each spot in one DNA chip, and the vertical axis represents the detection intensity of each spot in the other DNA chip.

[0136] Regarding its results, such as Figure 11 As shown, it was confirmed that there was no large difference in detection intensity between the two DNA chips and it was normal. Additionally, if Figure 12 As shown, there are uneven spots on the inner side, but when the median is used, it is considered that it can be used sufficiently as data and cannot be excluded.

[0137] Next, among these spots, when the detection intensity i...

Embodiment 2

[0144] Hereinafter, Example 2 in which appropriate offset values ​​are studied will be described. In Example 2, two chips of 3D-Gene (registered trademark) Human Ver1.1 (manufactured by Toray) were used as carriers. In addition, human reference RNA manufactured by Stratagene was used as a test substance, and it was carried out as described in the protocol until hybridization. In addition, 3D-Gene (registered trademark) Scanner (manufactured by Toray) was used as a detection means.

[0145] For one of the two hybridized chips, the setting of the photomultiplier tube (PMT) of the 3D-Gene (registered trademark) Scanner (manufactured by Toray) was changed to 40%, 55%, and 70% for measurement, For each spot, the values ​​of Expression 1 and Expression 2 were calculated. If the setting of the PMT is changed, the control voltage of the PMT changes proportionally. Therefore, the gain of the photomultiplier tube also changes. here, Figure 15 For a scatter plot showing the results...

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Abstract

The problem to be solved by the present invention is to provide a method for specifying the position of a speckle in image data obtained by imaging the detected intensity in a carrier, extracting a pixel group corresponding to the speckle, and calculating the detected intensity in the extracted pixel group. The ratio or difference between the median value and the median value of the detected intensities in the group after removing the upper predetermined proportion and / or the lower predetermined proportion of pixels from the pixel group is based on the calculated ratio or difference and the predetermined reference Value, to determine the reliability of good or bad.

Description

technical field [0001] The present invention relates to a determination method, a determination device, a determination system, and a program. Background technique [0002] In recent years, with the development of technologies such as microarray experiments and macroarray experiments, it is possible to comprehensively analyze a huge amount of genes, proteins, and the like. For example, in a DNA microarray, hundreds to tens of thousands of DNAs are arranged in a matrix on a carrier such as a glass slide in advance and immobilized as spots. Hybridization is performed so that the level of gene expression can be determined. [0003] That is, since a test substance such as labeled cDNA selectively binds to the complementary DNA on the carrier, the detection intensity of the label is obtained, and the gene expression level can be estimated. Here, although the selective binding amount of the test substance such as gene expression level is required to be reliable as data, for exam...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N33/53G01N37/00
CPCG01N33/5308G01N21/6458G06T7/0012G06T2207/10056G06T2207/10064G06T2207/30072G06V20/695
Inventor 薙野邦久佐佐本裕方铃江茂
Owner TORAY IND INC
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