A kind of long sporogenous yeast and its application

A technology of S. longiflorum yeast and shredded tobacco, which is applied in the field of applied microorganisms, can solve the problems that have not yet been found in public reports, and achieve the effects of promoting the healthy development of the cigarette industry, improving the grade and quality of tobacco leaves, and accelerating fermentation

Active Publication Date: 2016-09-07
CHINA TOBACCO YUNNAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But through document retrieval, do not find yet the public report of identical document with the content of the present invention

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  • A kind of long sporogenous yeast and its application
  • A kind of long sporogenous yeast and its application
  • A kind of long sporogenous yeast and its application

Examples

Experimental program
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Embodiment 1

[0025] - Collection of test tobacco leaf samples

[0026] The tobacco leaf samples used in this study were all collected from the tobacco aging workshop of the raw material department of Hongyun Tobacco (Group) Co., Ltd. The specific conditions of the tobacco leaves are shown in Table 1.

[0027] Table 1. Tobacco leaf samples tested

[0028]

[0029] -The cultivation method of L. elongisporus OF-08 (L. elongisporus OF-08).

[0030] 1) Test tube slant culture

[0031] Yeast Extract Peptone Dextrose Medium (YPD) medium was used, and the formula was (gram / liter, g / v): 20 grams of peptone, 20 grams of glucose, 10 grams of yeast extract, 20 grams of agar, and distilled water to 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate L.elongisporus OF-08 and culture at 28°C for 24 hours to obtain test tube seeds.

[0032] 2) Preparation of bacterial agent

[0033] A. Seed culture (YPD medium), the formula is (gram / liter, g / v): 20 grams of peptone, 20 grams...

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Abstract

The invention discloses a longosporin yeast and its application, belonging to the technical field of microbial application. The bacterial strain of the present invention is classified as Lodderomyces elongisporus OF-08; it was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on October 8, 2012, and the preservation number is CGMCC No.6645. The physiological and biochemical characteristics of the OF-08 strain and the 26S rDNA gene sequence (the sequence number of this sequence in the GeneBank database is JQ916053) are the main characteristic basis for identifying the strain. Spraying the strain preparation can increase the aroma quality and reducing sugar content of tobacco leaves, and decrease the content of protein, cellulose, total nitrogen and nicotine. Therefore, the ratios of sugar-to-nitrogen ratio, sugar-to-alkaline ratio and nitrogen-to-alkaline ratio tend to increase, which makes the chemical quality more harmonious and improves the smoking quality of tobacco leaves.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms, and more specifically, the invention relates to a spore-forming yeast capable of improving the quality of flue-cured tobacco. Simultaneously, the present invention also relates to the use of the said S. longitum in tobacco production. Background technique [0002] Tobacco is one of the important pillars of our country's finances. In the past two decades, tobacco taxation accounts for about 10% of the country's fiscal revenue every year, and has made great contributions to my country's social and economic development. In order to promote the progress of tobacco in our country and realize the sustainable development of the cigarette industry, we must take the road of tobacco technology innovation. The State Tobacco Monopoly Administration clearly stated in Article 7 of the "Eleventh Five-Year" China Cigarette Science and Technology Development Outline that it is necessary to "overc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A24B15/20C12R1/645
Inventor 段焰青冯斌杨培香者为夏建军朱保昆党立志王文元
Owner CHINA TOBACCO YUNNAN IND
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