Clostridium butyricum and its application

A technology of Clostridium butyricum and strains, applied in the fields of application, bacteria, microorganisms, etc., can solve the problems of few research reports on Clostridium butyricum and less application research, so as to improve animal production performance, improve the structure of flora, The effect of reducing usage

Active Publication Date: 2014-11-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] There are not many domestic research reports on Clostridium butyricum, and the research mainly focuses on

Method used

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  • Clostridium butyricum and its application
  • Clostridium butyricum and its application
  • Clostridium butyricum and its application

Examples

Experimental program
Comparison scheme
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[0031] Implementation case 1 Isolation and identification of strains

[0032] 1. Experimental method

[0033] 1.1 Experimental materials 10 pieces of healthy piglet manure from a pig farm in Zhejiang Province

[0034] 1.2 Medium

[0035] ① Clostridium proliferation medium (both mass concentration g / L): tryptone 20, beef extract 10, yeast extract 6, glucose 4, taurocholate 5, compound salt 1 part (K 2 HPO 4 2. KH 2 PO 4 1. MgSO 4 0.4, CaCl 2 0.2, FeSO 4 0.1, cysteine ​​hydrochloride 0.5, the same below); pH7.2, 121℃, 15min sterilization

[0036] ② Clostridium counting medium (all mass concentration g / L): Tryptone 10, Beef Extract 5, Yeast Extract 3, Glucose 4, Agar 16; One part of complex salt, pH7.2, 121℃, 15min sterilization

[0037] ③ Clostridium culture medium (both mass concentration g / L): Tryptone 20, Beef Extract 10, Yeast Extract 6, Glucose 4, 1 part of complex salt; pH 7.2, 121°C, 15min sterilization

[0038] 1.3 Specific experimental methods

[0039] 1) Take a stool sample and add...

Example Embodiment

[0058] Implementation case 2

[0059] Clostridium butyricum preparation (Clostridium butyricum) ZJU-F1 and its microbial compound preparation instead of antibiotics to reduce the application of zinc oxide in weaned piglets

[0060] Purpose and significance: Weaned piglets were selected as the test subjects to explore the effects of Clostridium butyricum microecological preparations on the growth performance, diarrhea rate, and intestinal microecology of weaned piglets under the conditions of no antibiotics and low-dose zinc oxide. Probiotics can reduce the impact of antibiotics and high-dose zinc oxide applications on animal growth performance, alleviate weaning stress, and intestinal microflora.

[0061] Test animals: 120 healthy piglets of 23±2 days old Du×Long×Large hybrids were selected, weighing 7.8±0.2Kg.

[0062] Clostridium butyricum preparation: Clostridium butyricum (Clostridium butyricum) ZJU-F1 is spray-dried after liquid fermentation, corn meal is the carrier, and the num...

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Abstract

The invention discloses a Clostridium butyricum and its application. The Clostridium butyricum is Clostridium butyricum ZJU-F1 specifically, is separated from the intestinal tract of a healthy piglet, and has strong stress resistance and high temperature resistance. A fermentation product is emulsified to prepare a Clostridium butyricum preparation, and the preparation can used for preparing animal feeds as a feed additive, and can substitute antibiotics or/and reduce the use of zinc oxide. The above micro-ecological preparation can regulate the micro-ecological balance in the intestinal tracts of animals, promotes the growth of beneficial bacteria, enhances the nonspecific immune function of animals, secretes trophic factors and protease, promote s the digestion and absorption of nutrients, and promotes animal production to improve the feed pay.

Description

technical field [0001] The invention relates to the field of feed additive-microbial preparations, in particular to a high-efficiency and safe Clostridium butyricum and its application. Background technique [0002] Feed antibiotics have significant effects in promoting animal growth, improving feed conversion efficiency, reducing food loss, and increasing pork production. However, with the widespread use of antibiotics as animal growth-promoting agents in feed, its disadvantages are becoming increasingly prominent, such as causing animal gastrointestinal flora imbalance, drug resistance and drug residues, etc., causing a series of public safety and environmental issues. Conservation and livestock product quality issues. It has become one of the hotspots in the field of animal nutrition to search for green and safe feed additives that are non-toxic, efficient, and do not produce drug resistance. [0003] Feed microbial preparations are beneficial to balance the intestinal ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/145A23K10/18A23K50/30A23K50/60
Inventor 汪以真王腾浩
Owner ZHEJIANG UNIV
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