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Anti-stress agent for piglet and preparation method of anti-stress agent

An anti-stress, piglet technology, applied in the direction of anti-toxic agent, application, digestive system, etc., can solve the problems of ineffective effect, unstable anti-stress effect, etc., achieve good anti-stress effect, inhibit intestinal harmful bacteria, The effect of improving immunity

Inactive Publication Date: 2014-12-10
HUNAN DANWEI BILOGICALTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these patented products have certain effects on piglets' anti-stress, many products are limited to Chinese herbal medicine or its extracts, vitamins and other compositions, and have shortcomings such as unstable anti-stress effects and ineffective effects

Method used

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  • Anti-stress agent for piglet and preparation method of anti-stress agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The strains used in this example are Clostridium butyricum, Pediococcus lactis, and Bacillus natto. The steps are:

[0032] (1) Preparation of liquid strains: inoculate a ring of slant strain cells with 1 strain of Bacillus natto in the nutrient medium of liquid expansion broth, and culture on a shaker at 36°C and 160r / min for 22 hours to make the strain The concentration of bacteria in the seed culture solution reached 5.8×10 8 cfu / mL;

[0033] Inoculate a ring of slant strain cells with a strain of Pediococcus lactis in liquid MRS medium, and culture it statically at 37°C for 36 hours, so that the bacterial concentration in the seed culture solution reaches 3.7×10 8 cfu / mL;

[0034] Inoculate a ring of slanted strain cells of Clostridium butyricum in the liquid seed medium of Clostridium butyricum, and cultivate in an anaerobic incubator at 35°C for 96 hours, so that the bacteria in the seed culture solution The concentration reaches 2.6×10 7 cfu / mL.

[0035] (2...

Embodiment 2

[0042] The strains used in this example are Clostridium butyricum, Pediococcus lactis, and Bacillus natto. The steps are:

[0043] (1) Inoculate one ring of Bacillus natto slant strain cells into the liquid expansion broth nutrient medium, and culture it on a shaking table at 37°C and 170r / min for 20h to make the bacteria in the seed culture solution body concentration reaches 6.5×10 8 cfu / mL; Inoculate a ring of slanted strain cells of Pediococcus lactis into liquid MRS medium, and culture it statically at 40°C for 48 hours, so that the bacterial concentration in the seed culture solution reaches 4.05×10 8 cfu / mL; Inoculate a ring of slanted strain cells of Clostridium butyricum in the liquid seed medium of Clostridium butyricum, and cultivate it in an anaerobic incubator at 32°C for 104 hours to make the seed culture The concentration of bacteria in the liquid reached 2.2×10 8 cfu / mL.

[0044] (2) Take wheat bran 31.5%, corn flour 24%, rice flour 18.2%, zeolite powder 23...

Embodiment 3

[0049] The strains used in this example are Clostridium butyricum, Pediococcus lactis, and Bacillus natto. The steps are:

[0050] (1) Inoculate one ring of Bacillus natto slant strain cells into the liquid expansion broth nutrient medium, and culture it on a shaking table for 24 hours at 35°C and 180r / min to make the bacteria in the seed culture solution body concentration reaches 4.2×10 9 cfu / mL; Inoculate a ring of slanted strain cells of Pediococcus lactis into liquid MRS medium, and culture it statically at 38°C for 40 hours, so that the bacterial concentration in the seed culture solution reaches 3.93×10 7 cfu / mL; Inoculate a ring of slanted strain cells of Clostridium butyricum in the liquid seed medium of Clostridium butyricum, and culture it in an anaerobic incubator at 30°C for 120 hours to make the seed culture The concentration of bacteria in the liquid reached 3.12×10 7 cfu / mL.

[0051] (2) Take wheat bran 38.5%, corn flour 20.5%, rice flour 16.7%, zeolite pow...

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Abstract

The invention discloses an anti-stress agent for a piglet and a preparation method of the anti-stress agent. The preparation method comprises the following steps: mixing butyrate clostridium powder, lactic acid bacteria powder, bacillus natto powder, vitamin C and / or vitamin E phosphate, organic acid and sylvite, and uniformly stirring. The butyrate clostridium powder, the lactic acid bacteria powder and the bacillus natto powder are prepared by adopting the steps of inoculating various strain liquid seeds in a solid culture medium, fermenting, drying and crushing. The anti-stress agent disclosed by the invention is capable of helping intestinal tracts of the piglet rapidly establish microecological balance, and capable of improving the immunity of the piglet at delactation stage and preventing the stress syndromes of the piglet occurring in ablactation, crowd transferring, transporting and the like, and has the characteristics of good stress resisting effect, wide raw material source, simple production process, low cost, no three-waste (waste gas, waste water and industrial residue) emission and the like.

Description

[0001] technical field [0002] The invention belongs to the field of biological technology in animal breeding, and in particular relates to a compound microecological antistress agent for piglets and a preparation method thereof. Background technique [0003] Porcine stress syndrome refers to a series of neuroendocrine non-specific systemic reactions produced when the pig body is in a state of tension or alert after being strongly stimulated by various stress factors, and the resulting various diseases of the body. Changes in functional and metabolic functions. Examples include impaired growth, decreased disease resistance, reproductive failure, and even death. In recent years, due to the rapid development of my country's pig industry, the introduction and popularization of foreign lean-meat pig breeds, and the rise of intensive and large-scale pig farming, long-distance transportation, high stocking density, and frequent human interference have caused pigs to respond to s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20A61K35/74A61P1/00A61P37/04A61P39/00A61P39/06
Inventor 曾梦良兰时乐
Owner HUNAN DANWEI BILOGICALTECH
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