Improving drought resistance in plants: UPL3
A technology of drought resistance and plants, applied in plant products, plant genetic improvement, testing plants/trees, etc., can solve problems such as growth retardation
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Embodiment 1
[0128] Embodiment 1 Drought experiment
[0129] Functional UPL3 protein produced by transformation of Arabidopsis (At) seeds with the Agrobacterium tumefaciens vector pROK2 was obtained from the Nottingham Arabidopsis Seed Center (NASC; School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, LE125RD United Kingdom) Deleted (NASC ID: N670558, AGI codes At4g38600 and SALK_037636C; hereinafter referred to as mutant seeds or mutant plants). At Col-0 (Columbia, N60000) was used as a control; hereinafter referred to as control seeds or plants.
[0130] Growth medium:
[0131] A soil mixture containing one part sand and vermiculite to two parts compost (sand:vermiculite:compost = 1:1:2) was used. The mixture increases water penetration, thus promoting even water absorption and better drainage in each pot. Seeds were stratified by keeping at 4°C for 3 days in dark and humid conditions before sowing.
[0132] Both mutant and control seeds were plan...
Embodiment 2
[0136] Embodiment 2 Drought experiment
[0137] Growth medium:
[0138] In the pre-screening experiments, the same mutant and control plants as in Example 1 were grown in a similar tray setup as described above. Plants were stressed by interruption of water supply from 15DAG until control plants reached their PWP. During this period, every other day, the pots were shuffled within the tray to reduce position effects and allow for uniform evaporation. On day 15 DOD, the control plants reached PWP and did not recover when water was added. Starting at 15DOD, one pot replicate from the mutant was watered daily and checked for drought stress recovery. Take pictures and rate the recovery. The mutants showed recovery from drought stress at least 3 days after the control reached its PWP.
[0139] figure 1 Shown are pictures comparing mutants and controls (left), indicating the superior effect of the mutants (right column) with regard to resistance to drought stress.
Embodiment 3
[0140] Embodiment 3 Drought experiment
[0141] Plant material. A TDNA insertion line with a disrupted AT4G38600 gene (SALK_037636C) was obtained from the Nottingham Arabidopsis Seed Center (NASC). Complementation lines were generated by stably transforming Arabidopsis plants using floral dip transformation (Bent et al., 2006. Methods Mol. Biol. Vol. 343:87-103). Homologs of the Arabidopsis (AT4G38600) UPL3 gene were identified from several crop species including tomato (tomato) and rice, and the model species Arabidopsis (UPL4; AT5G02880).
[0142] Table 1. Homologs of the Arabidopsis UPL3 gene
[0143]
[0144] Table 2. Arabidopsis UPL3 cDNA sequence (SEQ ID NO:1) and Arabidopsis (At5g02880(UPL4)); SEQ ID NO:3), tomato (Slg98247; SEQ ID NO:7 and Slg81026; SEQ ID NO:5 ) and rice (Os05g03100; SEQ ID NO:9) the percentage of nucleotide sequence identity between the homologue cDNA sequence (first column); and Arabidopsis UPL3 protein sequence (SEQ ID NO:2) and Arabidopsis ...
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