Composition of anticancer agent using extract or active substance thereof

A kind of technology of Aphrodisiac sativa and its composition, which is applied in the field of anticancer agent composition

Active Publication Date: 2016-06-08
济州岛科技园
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently used anticancer agents can be roughly divided into biological agents such as enzyme preparations or vaccines, chemically synthesized drugs, and drugs from natural organisms. Among them, biological agents such as enzymes and vaccines have not yet been applied, while chemically synthesized drugs Since the drug has different pharmacological effects depending on the type of cancer (Gillman., et al., Maxwell Macmillan. 18, pp1202, 1986), side effects due to toxicity appear, so there are problems in the treatment of cancer (Chung., et al., J. Wonkwang Medical Sci., 3, pp13-34, 1987)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composition of anticancer agent using extract or active substance thereof
  • Composition of anticancer agent using extract or active substance thereof
  • Composition of anticancer agent using extract or active substance thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Manufacture of extracts and fractions of A.

[0059] Put 1 kg of finely chopped Azalea (whole herb) into 80% ethanol, soak for 48 hours, extract repeatedly three times, filter to obtain the extract, concentrate the extract under reduced pressure to remove the solvent, and freeze-dry to obtain a powder extract.

[0060] In addition, after suspending and dissolving the obtained ethanol extract in 10 wt. 2 Cl 2 ), ethyl acetate (EtOAc) butanol (BuOH) for fractional distillation to obtain fractions of a hexane layer, a dichloromethane layer, an ethyl acetate layer, a butanol layer and the remaining aqueous layer.

Embodiment

[0062] Hereinafter, the present invention will be described with reference to examples and experimental examples. However, the scope of the present invention is not limited by these Examples and Experimental Examples.

[0063] Manufacture of the extract and fraction of A. sativa and the separation of its effective substances

[0064] Manufacture of extracts and fractions of A.

[0065] Put 1 kg of finely chopped Azalea (whole herb) into 80% ethanol, soak for 48 hours, extract repeatedly three times, filter to obtain the extract, concentrate the extract under reduced pressure to remove the solvent, and freeze-dry to obtain a powder extract.

[0066] In addition, after suspending and dissolving the obtained ethanol extract in 10 wt. 2 Cl 2 ), ethyl acetate (EtOAc) butanol (BuOH) for fractional distillation to obtain fractions of a hexane layer, a dichloromethane layer, an ethyl acetate layer, a butanol layer and the remaining aqueous layer.

Embodiment 2

[0067] Separation and identification of effective substances

[0068] 8 g of the above-mentioned ethyl acetate fraction was mixed with Celite 545, and the resulting mixture was concentrated under reduced pressure, followed by open column chromatography. Using n-Hex., MC (methylenechloride, dichloromethane), DE (Diethylether, diethyl ether), EA (Ethylacetate, ethyl acetate), Methanol (MeOH, methanol) as mobile phases, a total of five small fractions were obtained. However, silica gel column chromatography was performed on the DE (800 mg) fraction. Using chloroformate / methanol (9 / 2) as mobile phase at a flow rate of 2.5 ml / min, a total of 18 small fractions were obtained. The No. 14 fraction (DE-14) was simply purified to obtain a single compound (compound1).

[0069] To analyze the structure of the isolated substance, 10 mg of a single compound was dissolved in methanol-d to measure NMR spectrum. In 1HNMR spectrum ( figure 1 and image 3) in which three methyl groups we...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention provides an anticancer agent composition using an extract of A. japonicus or its active substance, which exhibits anticancer activity in cell experiments and animal experiments.

Description

technical field [0001] The present invention relates to an anticancer drug composition using an extract of Adonisamurensis or its active substance. Background technique [0002] In general, cancer refers to a malignant tumor in which the cycle of cells constituting human tissues is abnormal, resulting in abnormal differentiation of cells, regulation of growth, and enlargement of cells. [0003] According to the known situation, cancer occurs through three steps of initiation, promotion, and progression. Cell mutations are caused by carcinogens contained in the environment or food. Such cells survive the persistence of carcinogens. Under the stimulation, abnormal proliferation continues to form cancerous tissue. [0004] The research methods of anticancer agents for the treatment of cancer include the method of searching for direct cytotoxic substances to cancer cells, the method of searching for substances that can regulate the immune ability of the human body, the method o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/35A61P35/00A61K36/71A61K9/20
CPCA61K31/352A61K36/71A23L33/105A61P35/00A61K2300/00A61K9/20A61K31/35
Inventor 咸瑛旻金佶男郑龙焕尹源种朴修永吴大株
Owner 济州岛科技园
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap