Method for improving preservation effect of cymbidium type protocorm
A kind of protocorm and Cymbidium-like technology is applied in the field of improving the preservation effect of Cymbidium-like protocorms, which can solve the problems of insufficient production and scientific research, and achieve the improvement of relative survival rate, the optimization of preservation effect, and the improvement of viscosity and The effect of thermal conductivity
Inactive Publication Date: 2015-01-07
SHANGHAI JIAO TONG UNIV
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Problems solved by technology
The relative survival rate of the vitrified cryopreservation system of Cymbidium protocorms that has been initially established is only 10%, which is not enough to meet the needs of production and scientific research
Method used
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Experimental program
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Effect test
Embodiment 1
[0047] 1) Precultivating Cymbidium protocorms under light for 5 days on MS solid medium containing 60g / L sorbitol;
[0048] 2) Transfer to the loading solution and soak at room temperature for 40 minutes;
[0049] 3) Transfer to vitrification solution and dehydrate at 0°C for 50 minutes;
[0050] 4) Finally, store in liquid nitrogen at ultra-low temperature.
[0051] After step 3), without removing the vitrification solution, the Cymbidium-like protocorms soaked in the vitrification solution are directly stored in liquid nitrogen at ultra-low temperature.
[0052] Cymbidium protocorms were divided into experimental group and control group according to the above steps.
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The invention discloses a method for improving a preservation effect of cymbidium type protocorm. According to the invention, the cymbidium type protocorm is processed by adopting a vitrification solution which contains a carbon nanometer material so as to improve the preservation effect of the cymbidium type protocorm. The method specifically comprises the steps of pre-culture, contained liquid treatment, vitrification solution treatment and liquid nitrogen preservation, wherein the vitrification solution contains 0.1-0.5 g / L of carbon nano tubes. The method disclosed by the invention optimizes the preservation effect of the cymbidium type protocorm remarkably; as the carbon nano tubes are added to serve as an allogenic material, the vitrification ultra-low temperature preservation of plants is facilitated.
Description
technical field [0001] The invention relates to the field of preservation of plants or parts thereof, in particular to a method for improving the preservation effect of protocorms of Cymbidium grandis. Background technique [0002] Cryopreservation is a modern in vitro preservation technology for germplasm resources developed in the 1970s. Usually stored in liquid nitrogen, the substance metabolism and growth activities in the preserved material cells are almost completely stopped, and they are in a relatively stable biological state, achieving the purpose of long-term preservation of germplasm. Ultra-low temperature preservation is currently the only method that does not require continuous subculture. Medium and long-term preservation method. Vitrification cryopreservation is to place cells or tissues in a vitrification solution composed of a certain proportion of permeable and non-permeable protective agents, so that the material and its vitrification solution solidify in...
Claims
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Inventor 张亚非王路尧任丽陈冠群申晓辉
Owner SHANGHAI JIAO TONG UNIV
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