Method for preparing seaweed product for reducing blood pressure via step-by-step extraction and bioconversion coupling
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A biotransformation and blood pressure-lowering technology, applied in peptide preparation methods, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problem of high-value utilization of kelp, low economic value, step-by-step operation Complicated problems, to achieve the effect of improving high-value utilization, shortening extraction time, and improving extraction efficiency
Active Publication Date: 2015-01-07
FUZHOU HAILIN FOODSTUFF
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This patent basically realizes the comprehensive utilization of kelp, and obtains the mixed crude extract of kelp's nutritional components, but has not yet obtained a specific single component, and has not been able to study and utilize the specific effects of nutrient components. The economic value is not high enough to Realize the high-value utilization of kelp, and the steps are complicated and consume a lot of energy
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Embodiment 1
[0026] A method for preparing kelp blood pressure lowering products by coupling stepwise extraction and biotransformation includes the following steps:
[0027] 1) Raw material pretreatment: Fresh kelp or kelp scraps are cleaned and removed, dried at a low temperature of 60°C to constant weight, and crushed into 80 mesh powder for use.
[0028] 2) Alcohol extraction of mannitol: the powder and 95wt.% ethanol in step 1) are subjected to cyclic ultrasonic assisted extraction at a solid-liquid ratio of 1:30, time 55min, temperature 50°C, vacuum suction filtration, vacuum drying of the filter residue for use The filtrate is concentrated and filtered while it is hot, crystallized at 0°C, filtered and dried to obtain the kelp mannitol product;
[0029] 3) Acid extraction of algin and fucoidan: 2) the filter residue and 0.1mol·L -1 The hydrochloric acid solution is subjected to cyclic ultrasonic assisted extraction with a solid-liquid ratio of 1:50, for 45 minutes, at a temperature of 60°C,...
Embodiment 2
[0059] A method for preparing kelp blood pressure lowering products by coupling stepwise extraction and biotransformation includes the following steps:
[0060] 1) Raw material pretreatment: fresh kelp or kelp scraps are cleaned and removed, dried at a low temperature of 60°C to constant weight, crushed into 100 mesh powder for use.
[0061] 2) Alcohol extraction of mannitol: the powder and 95wt.% ethanol in step 1) are subjected to cyclic ultrasonic-assisted extraction at a solid-liquid ratio of 1:25, time 60min, temperature 50℃, vacuum suction filtration, and vacuum drying of the filter residue for use The filtrate is concentrated and filtered while it is hot, crystallized at 0°C, filtered and dried to obtain the kelp mannitol product;
[0062] 3) Acid extraction of algin and fucoidan: 2) the filter residue and 0.1mol·L -1 The hydrochloric acid solution is subjected to cyclic ultrasonic-assisted extraction with a solid-liquid ratio of 1:35 for 50 minutes, at a temperature of 5...
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Abstract
The invention relates to a health care product for assisting blood pressure reduction, provides a method for preparing a health care seaweed product for assisting blood pressure reduction via step-by-step extraction and bioconversion coupling, and belongs to the field of deep processing of ocean foods. The method is carried out by taking high-quality seaweeds as raw materials via an ultrasonic circulation step-by-step extraction process and a bioconversion coupling preparation technology. The preparation method sequentially comprises the following steps: carrying out pretreatment of raw materials, alcohol extraction of mannitol, acid extraction and alcohol precipitation of sodium alginate and fucoidan, bioconversion and ultrafiltration so as to obtain physiological active components for reducing blood pressure; and scientifically blending the active components. According to the method, a large quantity of seafood seaweeds in China are deeply processed and comprehensively utilized so as to obtain five products, namely mannitol, sodium alginate, fucoidan, seaweed dietary fibers and seaweed antihypertensive peptides; and the five products are scientifically blended so as to develop a series of novel functional seaweed products for reducing blood pressure reduction, wherein the series of functional seaweed products have obvious functions of assisting blood pressure reduction. The ACE inhibitory activity IC50 of the seaweed product is within 0.68mg/g to 1.15mg/g, so that the blood pressure of an SHR (Spontaneously Hypertensive Rat) can be obviously lowered (-12.4 mmHg to -17.5mmHg).
Description
technical field [0001] The invention belongs to the field of deep processing of marine food, and in particular relates to a method for preparing kelp products for auxiliary blood pressure lowering and health care through step-by-step extraction and biotransformation coupling. Background technique [0002] Hypertension is an important risk factor for cardiovascular and cerebrovascular diseases, and has become a serious international public health problem. However, most of the antihypertensive drugs sold on the market are chemically synthesized drugs, and long-term use is likely to produce corresponding side effects, causing physical discomfort. Therefore, it will be very important to develop a food-derived hypotensive product with high safety, few side effects and easy absorption. [0003] Kelp is rich in nutrients and has many functions. It is known as the "crown of alkaline plants" and has high economic value. Lowering blood fat and anti-cancer, etc., are valuable raw mat...
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