The invention discloses a preparation method of 
ceramide. The preparation method comprises the following steps: step 1, adding 
ethanol into konjac flying 
powder, carrying out stirring and filtering toobtain an 
ethanol extracting solution and a filter residue for later use; step 2, concentrating the 
ethanol extracting solution to obtain a concentrated paste; step 3, adding 
potassium hydroxide intothe concentrated paste, carrying out stirring to obtain a stirred liquid, adding 
petroleum ether into the stirred liquid, and carrying out extracting and concentrating to obtain a crude 
ceramide product; and step 4, adding an 
organic solvent into the crude 
ceramide product for 
dissolution, carrying out eluting by adopting an eluting agent to obtain an eluent, and carrying out 
drying to obtain theceramide. According to the preparation method, soaking is carried out by using the ethanol for removing peculiar-smell substances in the konjac flying 
powder, 
solid-liquid separation is carried out by adopting 
filtration, then an obtained 
solid is dried and used as feed, and an obtained liquid is used for preparing ceramide. Acidic substances such as fatty acids and the like in the crude ceramideproduct is neutralized through the addition of 
potassium hydroxide, so that the ceramide is promoted to exist in a 
free form, the purity of the prepared ceramide can reach 96% or above, and the use amount of organic reagents is low.