Preparation method of ceramide

A technology of ceramide and ethanol, which is applied in the field of extraction of plant active ingredients, can solve problems such as not being suitable for large-scale production, and achieve the effects of increasing product added value, rapid separation, and simple methods

Pending Publication Date: 2018-12-07
三原利华生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this method, a medium-pressure C18 column is used, and the medium-pressure C18 is only suitable for small-scale preparation, not suitable for large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of ceramide, comprising the following preparation steps:

[0033] Step 1, take 5000g of konjac powder, add 90% ethanol with 5 times the mass of konjac powder, stir at room temperature for 2 hours to extract, extract twice, filter to obtain ethanol extract and filter residue, and set aside.

[0034] Step 2: Concentrate the ethanol extract for 3.5 hours at 47.5°C to obtain 147g of concentrated cream.

[0035] Step 3, take 100g of concentrated paste, add 106mL of 5% potassium hydroxide, stir at 20°C for 2 hours to obtain a stirred liquid, add petroleum ether with the same volume as the stirred liquid to the stirred liquid, and extract twice under normal temperature conditions, each The time of the second extraction was 2 hours. The petroleum ether phases after the two extractions were combined and concentrated at 42.5° C. for 2.5 hours to obtain 16.3 g of crude ceramide with a content of 49.3%.

[0036] Step 4, take 10 g of crude ceramide, add 30 ml o...

Embodiment 2

[0038] A preparation method of ceramide, comprising the following preparation steps:

[0039] Step 1, take 5000g konjac powder, add 95% ethanol with 3 times the mass of konjac powder, stir at room temperature for 3 hours to extract, extract twice, filter to obtain ethanol extract and filter residue, set aside.

[0040] Step 2, concentrate the ethanol extract at 45°C for 5 hours to obtain 165 g of concentrated paste.

[0041] Step 3, take 100g of the concentrated paste, add 101mL of 5% potassium hydroxide, stir at 35°C for 3 hours to obtain a stirred liquid, add petroleum ether with the same volume as the stirred liquid to the stirred liquid, and extract twice under normal temperature conditions, each The time of the second extraction was 3 hours. The petroleum ether phases after the two extractions were combined and concentrated at 40° C. for 3 hours to obtain 18.1 g of crude ceramide with a content of 46.9%.

[0042] Step 4, take 10 g of crude ceramide, add 30 ml of chlorofo...

Embodiment 3

[0044] A preparation method of ceramide, comprising the following preparation steps:

[0045] Step 1, take 5000g of konjac powder, add 99% ethanol with 5 times the mass of konjac powder, stir at room temperature for 5 hours to extract, extract twice, filter to obtain ethanol extract and filter residue, set aside.

[0046] Step 2, concentrate the ethanol extract at 50°C for 2 hours to obtain 167g of concentrated paste.

[0047] Step 3, take 100g of the concentrated paste, add 113mL of 5% potassium hydroxide, and stir at 50°C for 4 hours to obtain a stirred liquid. Add petroleum ether with the same volume as the stirred liquid to the stirred liquid, and extract twice under normal temperature conditions. The time of the second extraction was 1 hour. The petroleum ether phases after the two extractions were combined and concentrated at 40° C. for 3 hours to obtain 17.3 g of crude ceramide product with a content of 50.9% of crude ceramide.

[0048] Step 4, take 10g of crude ceramide...

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PUM

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Abstract

The invention discloses a preparation method of ceramide. The preparation method comprises the following steps: step 1, adding ethanol into konjac flying powder, carrying out stirring and filtering toobtain an ethanol extracting solution and a filter residue for later use; step 2, concentrating the ethanol extracting solution to obtain a concentrated paste; step 3, adding potassium hydroxide intothe concentrated paste, carrying out stirring to obtain a stirred liquid, adding petroleum ether into the stirred liquid, and carrying out extracting and concentrating to obtain a crude ceramide product; and step 4, adding an organic solvent into the crude ceramide product for dissolution, carrying out eluting by adopting an eluting agent to obtain an eluent, and carrying out drying to obtain theceramide. According to the preparation method, soaking is carried out by using the ethanol for removing peculiar-smell substances in the konjac flying powder, solid-liquid separation is carried out by adopting filtration, then an obtained solid is dried and used as feed, and an obtained liquid is used for preparing ceramide. Acidic substances such as fatty acids and the like in the crude ceramideproduct is neutralized through the addition of potassium hydroxide, so that the ceramide is promoted to exist in a free form, the purity of the prepared ceramide can reach 96% or above, and the use amount of organic reagents is low.

Description

technical field [0001] The invention relates to the field of extraction technology of plant active ingredients, in particular to a preparation method of ceramide. Background technique [0002] Konjac (jǔruò) (scientific name Amorphophallus konjac), commonly known as konjac, also known as konjac, is a perennial herb of the Araceae family, and it was also called demon taro in ancient China. Konjac contains 35% starch, 3% protein, and various vitamins, potassium, phosphorus, selenium and other mineral elements. It also contains konjac polysaccharides needed by humans, that is, glucomannan up to 30%. [0003] The nutritional and health care function of konjac is to play the role of dietary fiber in regulating nutritional imbalances, such as preventing and treating constipation, lowering blood fat, lowering blood sugar, weight loss and bodybuilding. In addition, it can also be used to make contact lenses and medical optical products; adding konjac glucomannan to eye care solutio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/24C07C233/20
CPCC07C231/24C07C233/20
Inventor 张永利罗庚寇玉锋
Owner 三原利华生物技术有限公司
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