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Method for in vitro somatic embryo induction and plant regeneration of blue spruce hoopsii

A somatic embryo, spruce technology, applied in the field of planting, can solve the problems of embryo abortion, unable to form natural seeds, unable to reproduce, etc., and achieve the effect of reasonable design, good market application prospect and promotion value

Inactive Publication Date: 2016-07-20
山西鑫中大生物科技有限公司 +2
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Problems solved by technology

However, the natural growth plants of this variety have serious embryo abortion problems, that is, this variety cannot form normal zygotic embryos and natural seeds under natural conditions, so it cannot be reproduced by seeds

Method used

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  • Method for in vitro somatic embryo induction and plant regeneration of blue spruce hoopsii
  • Method for in vitro somatic embryo induction and plant regeneration of blue spruce hoopsii
  • Method for in vitro somatic embryo induction and plant regeneration of blue spruce hoopsii

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Embodiment Construction

[0031] Specific embodiments of the present invention will be described in detail below.

[0032] The process of induction of somatic embryo formation and plant regeneration from immature zygotic embryos of naturally growing mother plants can be divided into the following stages:

[0033] The first stage: Embryogenic callus induction by suitable explant material.

[0034] The second stage: somatic embryo development and maturation. The development and maturation of somatic embryos in the present invention can be observed in four distinct stages: ① globe-shaped stage, ② torpedo-shaped stage, ③ early cotyledonary stage, and ④ cotyledon fully The mature embryo stage that develops and is ready for sowing (mature-embryostage).

[0035] The third stage: planting and germination of mature somatic embryos in vitro.

[0036] The fourth stage: the growth of test-tube seedlings, somatic embryos that have just germinated in 4-6 weeks can grow into 1-2cm high seedlings.

[0037] The fif...

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Abstract

This patent relates to plant propagation technology, specifically the selection of the best explant material collection time for immature sexual embryos in the field of the new blue spruce variety Hoopsii, the induction of somatic embryogenic callus in vitro, the in vitro maturation of somatic embryos, Somatic embryo germination in vitro, somatic embryo seedling transplantation and a series of supporting technologies. The present invention has successfully completed the whole process of germination and growth of the mature somatic cell embryos of this variety in test tubes until they grow into seedlings for the first time; Outdoor transplanting of large trees; providing a complete set of technologies for the large-scale factory production of future artificial seeds of the new species of Hoopsii blue spruce.

Description

technical field [0001] The invention relates to plant in vitro propagation technology, in particular to screening of optimal explant material collection time for immature sexual embryos of the new blue spruce variety Hoopsii in the field, optimal medium formula and optimal method for each link of in vitro somatic embryo induction and maturation. Screening of suitable culture conditions, including embryogenic callus induction, somatic embryo development and maturation, somatic embryo germination, test tube seedling growth and transplantation and a series of supporting technologies. Background technique [0002] Hoopsii is a budding variety of the blue spruce tree species, which was first reported in Canada in 1994. In the traditional blue spruce species, only the new leaves of the year are blue, and the old leaves after the second year of life begin to turn green. The new and old leaves of this variety are uniform blue, the tree body is beautiful and tall, and it is tower-sh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 毛静琴意利莫·欧塔萨阿拉·珀斯特崔增毅米文精刘英翠吕皎田建华张素华
Owner 山西鑫中大生物科技有限公司
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