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Preparation and application of electrochemiluminescence immunosensor for detecting tumor markers

A tumor marker, electrochemical technology, applied in the field of immune marker detection, can solve the problems of operators and immune substances, health hazards of operators, expensive instruments, etc., to facilitate on-site detection, reduce impact, The effect of enhancing conductivity

Inactive Publication Date: 2016-06-22
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these immunoassay methods generally take a long time and have cumbersome operations such as adding samples, incubating, and washing, and the operators have more contact with immune substances, which may cause harm to the health of the operators.
Moreover, some detection methods with fast determination speed, high sensitivity and low detection limit require expensive instruments and cannot be popularized in developing countries.

Method used

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  • Preparation and application of electrochemiluminescence immunosensor for detecting tumor markers
  • Preparation and application of electrochemiluminescence immunosensor for detecting tumor markers
  • Preparation and application of electrochemiluminescence immunosensor for detecting tumor markers

Examples

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preparation example Construction

[0034] A method for preparing an electrochemiluminescent sensor for detecting an immune marker, comprising the following steps:

[0035] (1) Cut the conductive glass into the required size and perform pretreatment;

[0036] (2) Use existing methods to polymerize polyaniline in the ITO square area and synthesize g-C 3 N 4 , Silver-gold core-shell nanospheres and metal oxides, metal oxides include zinc oxide, copper oxide, manganese dioxide;

[0037] (3) Carboxylated g-C 3 N 4 , immobilized on polyaniline with a linker for immobilizing the primary antibody;

[0038] (4) Silver-gold core-shell nanospheres are modified on the metal oxide, and then the secondary antibody is modified on the silver-gold core-shell nanospheres;

[0039] (5) Combine the prepared electrochemiluminescence sensor with an electrochemiluminescence instrument to detect tumor markers.

[0040] The polyaniline of the present invention / g-C 3 N 4 , the preparation of metal oxide / silver-gold and Konanometer...

Embodiment 1

[0053] Example 1 (embryonic antigens, such as alpha-fetoprotein, AFP)

[0054] (1) Select AFP with higher clinical incidence for determination;

[0055] (2) First cut the conductive glass into strips of 4cm×1cm, wash them with ethanol, acetone and secondary water for 15min in sequence, make a mold for vaseline printing template through Adobe Illustrator, and apply Vaseline on the ITO conductive glass specific area of

[0056] (3) Preparation of polyaniline: The preparation of polyaniline is prepared by electropolymerization. It is carried out in a three-electrode system composed of conductive glass as the working electrode, platinum electrode as the counter electrode, and Ag / AgCl electrode as the reference electrode. Composed of 4.00mL 3M nitric acid and 0.40mL aniline, the voltage applied on the working electrode is 0.1V·s -1 The scanning speed is cycled between -0.2-1.2V, thus producing polyaniline;

[0057] (4) g-C 3 N 4 Preparation: Weigh 10g of melamine in a crucib...

Embodiment 2

[0070] Example 2 (Carbohydrate markers, such as CA125)

[0071] (1) Select CA125 with higher clinical incidence for determination;

[0072] (2) First cut the conductive glass into strips of 4cm×1cm, wash them with ethanol, acetone and secondary water for 15min in sequence, make a mold for vaseline printing template through Adobe Illustrator, and apply Vaseline on the ITO conductive glass specific area of

[0073] (3) Preparation of polyaniline: The preparation of polyaniline is prepared by electropolymerization. It is carried out in a three-electrode system composed of conductive glass as the working electrode, platinum electrode as the counter electrode, and Ag / AgCl electrode as the reference electrode. Composed of 4.00mL 3M nitric acid and 0.40mL aniline, the voltage applied on the working electrode is 0.1V·s -1 The scanning speed is cycled between -0.2-1.2V, thus producing polyaniline;

[0074] (4) g-C 3 N 4 Preparation: Weigh 10g of melamine in a crucible and burn i...

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Abstract

The invention discloses a sensor for electrochemiluminescence detection of tumor markers. The preparation of the sensor includes the following steps: synthesizing polyaniline on conductive glass by electropolymerization; and preparing g-C3N4, silver-gold core-shell nanospheres and zinc oxide or copper oxide or manganese dioxide according to the existing method; G-C3N4 is connected to polyaniline with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide mixture as linking agent, and The primary antibody is modified on the conductive glass electrode prepared above; the surface of zinc oxide is covered with silver-gold core-shell nanospheres, and the secondary antibody is modified on the silver-gold nanospheres coated on the surface of zinc oxide; electrochemiluminescence is used for detection. The sensor of the invention has strong specificity, high sensitivity, simple operation, wide linear range and low detection limit.

Description

technical field [0001] The invention relates to the technical field of detection of immune markers, more specifically to the preparation of an electrochemiluminescence immunosensor, and also relates to a method for detecting tumor markers using the electrochemiluminescence sensor. Background technique [0002] For a long time, malignant tumors, also known as cancer, have been a serious threat to human health and life safety. Cancer cells refer to a new organism produced by malignant transformation and clonal proliferation of some cells with division potential under the action of carcinogenic factors. In addition to their own uncontrolled growth, cancer cells can also invade surrounding normal tissues and even transfer to other parts of the body through the body's circulatory system or lymphatic system, thereby causing body disease or even death. Therefore, early diagnosis and early treatment of cancer are of great significance. However, it is difficult to detect the low an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N21/76G01N27/26
CPCG01N21/66G01N21/76G01N33/574G01N33/57473
Inventor 颜梅王秀于京华申蕾邓文平葛慎光张彦刘海云杨春蕾
Owner UNIV OF JINAN