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A kind of rapid propagation method of Dendrobium fringe seed tissue culture

A technique for tissue culture of Dendrobium tassel, applied in the field of rapid propagation of Dendrobium tassel seed tissue culture, can solve problems such as artificial planting, and achieve the effects of saving energy, saving funds, and improving the efficiency of tissue culture

Active Publication Date: 2016-07-06
嘉兴芸诗娇电子商务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a rapid propagation method of Dendrobium fringe seed tissue culture, which directly takes the seeds of Dendrobium tassels for tissue culture, effectively shortens the cultivation cycle of Dendrobium tassels, reduces the variation probability caused by multiple subcultures, and uses tissue The obtained tissue cultured seedlings of Dendrobium fringe have robust plants and strong growth ability, and are suitable for industrial production to solve the seedling problem of artificial planting of Dendrobium fringe

Method used

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  • A kind of rapid propagation method of Dendrobium fringe seed tissue culture

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Embodiment 1

[0027] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.

[0028] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:

[0029] Step 1. Soak the fruits of Dendrobium tassels in 80% ethanol solution for 20 seconds, sterilize them with mercury chloride for 10 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, the cultivation temperature was 28°C, the light intensity was 2600 lux, and the light time was 15h / d, and cultured for 10 days to obtain small buds of Dendrobium nodii, wherein the induction medium was: MS-H Add 2.0 mg / L indole acetic acid, 2.0 mg / L naphthalene acetic acid, 0.5 mg / L thidi...

Embodiment 2

[0034] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.

[0035] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:

[0036] Step 1. Soak the fruits of Dendrobium tassels in 80% ethanol solution for 30 seconds, disinfect them with mercuric chloride for 20 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, cultured for 15 days under the condition that the culture temperature was 30°C, the light intensity was 2800 lux, and the light time was 14h / d, and the small shoots of Dendrobium fringe were obtained, wherein the induction medium was: MS-H Add 1.0 mg / L indole acetic acid, 3.0 mg / L naphth...

Embodiment 3

[0041] The invention discloses a rapid propagation method through tissue culture of Dendrobium fringe seeds. The seeds of Dendrobium tassels are taken as materials for in vitro induction culture to obtain tissue culture seedlings of Dendrobium tassels.

[0042] In the described Dendrobium fringe seed tissue culture rapid propagation method, comprise the following steps:

[0043] Step 1. Soak the fruits of Dendrobium tassels in 85% ethanol solution for 40 seconds, disinfect them with mercuric chloride for 20 minutes, cut the fruits of Dendrobium tassels after cleaning to obtain pretreated seeds of Dendrobium tassels, and sow the pretreated seeds of Dendrobium tassels in On the induction medium, cultured for 14 days at a culture temperature of 32°C, a light intensity of 3000 lux, and a light time of 13 h / d to obtain small buds of Dendrobium fringe, wherein the induction medium is: MS-H Basic medium, add 3.0mg / L indoleacetic acid, 4.0mg / L naphthaleneacetic acid, 1.0mg / L thidiazur...

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Abstract

The invention discloses a dendrobium fimbriatum seed tissue culture and rapid propagation method. Dendrobium fimbriatum seeds are sown in inducing culture media and induced to sprout, then dendrobium fimbriatum budlets are sequentially transplanted into successive transfer culture media and rooting culture media to continue to be cultured, and dendrobium fimbriatum rooting tissue culture seedlings are obtained and can be transplanted to a greenhouse for seedling hardening. The tissue culture method is simple and convenient, steps are easy, operation is convenient, dendrobium fimbriatum rooting tissue culture seedling plants are robust and have high growing capacity, and the method is suitable for factory production.

Description

technical field [0001] The invention relates to a method for rapid propagation of plants, in particular to a method for rapid propagation of dendrobium fringe seed tissue culture. Background technique [0002] Dendrobium is the fresh or dried stem of Dendrobium nobile Lindl., Dendrobium candidum Wall. ex Lindl. or Dendrobium fimbriatum Hook. var. oculatum Hook. and its similar species. In my country's traditional medicine, Dendrobium is a commonly used precious medicinal material, which was first recorded in "Shen Nong's Herbal Classic" and listed as top grade. [0003] Dendrobium fimbriatum Hook.var.oculatumHook. is a plant of the genus Dendrobium fimbriatum Hook.var.oculatumHook., with thick stems, oblique or drooping, hard texture, cylindrical or sometimes slightly spindle-shaped above the base, two rows of leaves, leathery, oblong or Oblong-lanceolate, racemes 5-15 cm long, 6-12 flowers sparsely, golden-yellow, thin, spreading, slightly fragrant, flowering period 4-6 mo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 霍丽妮苏钛陈睿李培源苏炜马静
Owner 嘉兴芸诗娇电子商务有限公司
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