A kind of platinum nanomaterial with four kinds of simulated enzyme activities and its preparation method

A platinum nanometer and enzyme-simulating technology, applied in the field of nanotechnology and bionics, can solve the problems of difficult purification, high price, harsh operating conditions, etc., and achieve the effects of simple and fast preparation process, small particle size, high dispersibility and stability

Inactive Publication Date: 2016-03-30
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the sources of natural enzymes are limited, difficult to purify, and expensive
At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications.

Method used

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  • A kind of platinum nanomaterial with four kinds of simulated enzyme activities and its preparation method
  • A kind of platinum nanomaterial with four kinds of simulated enzyme activities and its preparation method
  • A kind of platinum nanomaterial with four kinds of simulated enzyme activities and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Mix 1ml of 16mmol / L chloroplatinic acid aqueous solution with 1ml of 40mmol / L trisodium citrate, dilute with 38ml of double distilled water, and stir for 30 minutes in the dark. Then add 0.2ml of sodium borohydride aqueous solution with a concentration of 50mmol / L (the addition time is controlled within 2 minutes), the color of the reaction solution changes from light yellow to brownish yellow, and the stirring is continued for 1 hour in the dark. The concentration of the obtained platinum nanomaterial solution was 78mg / L, and it was stored at 4°C. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.

Embodiment 2

[0037] The solution obtained in Example 1 was drip-coated on a copper grid for detection by a transmission electron microscope. Such as figure 1 As shown, the average particle size of platinum nanoparticles is 2.5±0.4nm. Such as figure 2 As shown, the interplanar spacing of the obtained nanoparticles is 0.223 nm, which corresponds to the 111 crystal plane of the platinum crystal.

Embodiment 3

[0039] The solution obtained in Example 1 was freeze-dried to obtain a powder, and the obtained powder was subjected to X-ray photoelectron spectroscopy. Such as image 3 As shown, the obtained platinum nanoparticles consist of 86% Pt 0 and 14% Pt 4+ composition.

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Abstract

The invention discloses a Platinum nanomaterials with four kinds of simulated enzyme activities and their preparation methods, which are Citric acid-modified platinum nanomaterials were prepared by reducing chloroplatinic acid with sodium borohydride using citrate ion as a modifier. The preparation of platinum nanomaterials is simple and fast, the particle size is small, and the aqueous solution has high dispersibility and stability. The obtained platinum nanomaterial simultaneously has four kinds of simulated enzyme activities, including peroxidase, oxidase, catalase and superoxide dismutase. The four simulated enzyme activities of platinum nanomaterials can be tuned by the concentration of citrate ions used in the preparation process.

Description

technical field [0001] The invention relates to a platinum nano material with four simulated enzyme activities (peroxidase, oxidase, catalase, superoxide dismutase) and a preparation method thereof, belonging to the field of nanotechnology and bionic technology. Background technique [0002] Enzymes are very important proteins with catalytic activity in organisms. They have high catalytic efficiency, strong specificity, and mild reaction conditions. The growth, development, reproduction and other life activities of organisms are inseparable from the catalysis of enzymes. However, the sources of natural enzymes are limited, difficult to purify, and expensive. At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications. The artificial nano-enzyme has the characteristics of high c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/00B22F9/24B82Y30/00B82Y40/00
Inventor 陈伟何少斌吴钢伟邓豪华彭花萍
Owner FUJIAN MEDICAL UNIV
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