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A kind of preparation method of tetracycline

A technology for the preparation of tetracycline and strains, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of affecting product quality, long acidification time, and large equipment investment, so as to reduce production costs and work. quantity, and the effect of improving production efficiency

Active Publication Date: 2017-01-18
金河生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation method of tetracycline in the prior art has the following disadvantages: the use of flammable and explosive organic reagents with high storage costs; large investment in equipment, high production costs, low yield, and low economic benefits; repeated addition of water during the preparation process Dilution and acidification lead to long acidification time and easy to produce epimers, which seriously affect product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation method of tetracycline is as follows:

[0042] a. Seed cultivation

[0043] 1) Strain preparation

[0044] Streptomyces aureus spores were inoculated into agar slants containing spore medium (mother slants) and cultured at 34°C for 4 days. Then inoculate it on the seed slant and cultivate it for 5 days at 34°C until the spores mature;

[0045] The spore culture medium is composed of the following components in terms of mass percentage concentration: bran 3.0%, MgSO 4 0.005%, (NH 4 ) 2 HPO 4 0.01%, KH 2 PO 4 0.01%, agar powder 2%, and the balance is water. The composition of the sub-slant medium is the same as that of the mother slant medium;

[0046] 2) Seed tank culture

[0047] Inoculate the bacteria into the sterilized seed tank, cultivate for 25 hours at a culture temperature of 32°C, a tank pressure of 0.03MPa, a ventilation rate of 1:2.0v / v / min, and a stirring speed of 200rpm, and take samples for testing;

[0048] Test results: the seed...

Embodiment 2

[0074] The preparation method of tetracycline is as follows:

[0075] a. Seed cultivation

[0076] 1) Strain preparation

[0077] Streptomyces aureus spores were inoculated into agar slants containing spore medium (mother slants) and cultured at 34°C for 5 days. Then inoculate them on the seed slant and culture them at 34°C for 4 days until the spores mature.

[0078] The spore culture medium is composed of the following components in terms of mass percentage concentration: bran 3.4%, MgSO 4 0.005%, (NH 4 ) 2 HPO 4 0.015%, KH 2 PO 4 0.01%, agar powder 2.2%, the balance is water, the composition of the sub-slant culture medium is the same as that of the mother slant culture medium.

[0079] 2) Seed tank cultivation process

[0080] Inoculate the prepared strains into the sterilized seed tank, cultivate for 23 hours at a culture temperature of 33°C, a ventilation rate of 1:1.8v / v / min, and a stirring speed of 200rpm, and take samples for detection;

[0081] Test results...

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Abstract

The invention discloses a preparation method for tetracycline. The preparation method comprises the following specific steps of preparing a streptomyces aureus strain and then performing seed shake-flask culture and seed tank culture; when culture is performed for 20-26 hours, converting the culture mode into fermentation culture; when the pH value reaches 5.6-7.6, the total titer is not smaller than 8,000U / ml, the total saccharides content is not greater than 2.0 percent, the quantity of amino nitrogen is greater than 50mg / 100mL, and the filtering velocity is not smaller than 5mL / 5min, ending the fermentation and discharging from a tank; finally, filtering with a plate frame, decoloring ion exchange resin, crystallizing and performing air current drying to obtain the tetracycline. According to the preparation method disclosed by the invention, a fermentation process is simple, the fermentation unit can be effectively improved, the yield is improved, and the production cost is reduced; meanwhile, the use of chemical organic solvent is avoided.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a preparation method of antibiotics, in particular to a preparation method of tetracycline. Background technique [0002] Tetracycline is an antibacterial substance isolated from the culture solution of actinomycetes and Streptomyces aureus, etc. It has a good effect on Gram-positive bacteria, negative bacteria, rickettsia, viral viruses, spirochetes and even protozoa. Inhibition, its main mechanism is to destroy the formation of bacterial polysomes, interfere with protein synthesis, thereby inhibiting bacterial growth, and belongs to a broad-spectrum antibiotic. With the in-depth research on tetracycline antibiotics in the field of biopharmaceuticals, the types of tetracycline hydrochloride antibiotics with tetracycline hydrochloride as the core are increasing day by day. Level, improving the refining yield has become the core of the research. However, the preparation method of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P29/00C12R1/485
Inventor 谢昌贤邓维康刘运添王鹏飞
Owner 金河生物科技股份有限公司
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