A kind of preparation method of tetracycline

Abstract

The invention discloses a preparation method for tetracycline. The preparation method comprises the following specific steps of preparing a streptomyces aureus strain and then performing seed shake-flask culture and seed tank culture; when culture is performed for 20-26 hours, converting the culture mode into fermentation culture; when the pH value reaches 5.6-7.6, the total titer is not smaller than 8,000U / ml, the total saccharides content is not greater than 2.0 percent, the quantity of amino nitrogen is greater than 50mg / 100mL, and the filtering velocity is not smaller than 5mL / 5min, ending the fermentation and discharging from a tank; finally, filtering with a plate frame, decoloring ion exchange resin, crystallizing and performing air current drying to obtain the tetracycline. According to the preparation method disclosed by the invention, a fermentation process is simple, the fermentation unit can be effectively improved, the yield is improved, and the production cost is reduced; meanwhile, the use of chemical organic solvent is avoided.

Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals, and relates to a preparation method of antibiotics, in particular to a preparation method of tetracycline. Background technique

[0002] Tetracycline is an antibacterial substance isolated from the culture solution of actinomycetes and Streptomyces aureus, etc. It has a good effect on Gram-positive bacteria, negative bacteria, rickettsia, viral viruses, spirochetes and even protozoa. Inhibition, its main mechanism is to destroy the formation of bacterial polysomes, interfere with protein synthesis, thereby inhibiting bacterial growth, and belongs to a broad-spectrum antibiotic. With the in-depth research on tetracycline antibiotics in the field of biopharmaceuticals, the types of tetracycline hydrochloride antibiotics with tetracycline hydrochloride as the core are increasing day by day. Level, improving the refining yield has become the core of the research. However, the preparation method of...