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A kind of liquid film and preparation method thereof

A technology for liquid mulch and seeds, applied in the agricultural field, can solve the problems of poor biodegradability of liquid mulch, poor soil water and fertilizer retention capacity, and lower soil pH, and achieve excellent soil physical and chemical properties, increase crop yield, and improve soil. Effect

Active Publication Date: 2016-08-17
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is that the current soil salinization is serious, the use of chemical fertilizers causes soil compaction, the soil water and fertilizer retention capacity in some areas is poor, and the existing liquid mulch film has poor film production, biodegradability, and incomplete degradation, etc. Problem, using pullulan and polyglutamic acid as a liquid mulch, after spraying, it can increase soil temperature, inhibit water evaporation, increase soil water content, reduce soil salinity, and reduce soil pH

Method used

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  • A kind of liquid film and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A liquid mulching film, the weight percentage of each component is: pullulan 5%, polyglutamic acid 5%, and the balance is water.

[0038] The pullulan polysaccharide is prepared by the following method: fermentation medium composition (1L): sucrose 150g, peptone 5g, sodium chloride 3g, dipotassium hydrogen phosphate 7g, magnesium sulfate 0.4g, ferrous sulfate 0.5g, all the other are distilled water , initial pH5.5.

[0039] The CGMCC.NO.7055 strain was inoculated into the seed medium in an amount of 3% (v / v) for shake flask activation, the rotation speed was 200 rpm, the temperature was 28° C., and the incubation time was 32 hours. The activated seed culture solution was transferred to a new seed shake flask in an amount of 3% (v / v) for expanded cultivation, the rotation speed was 200 rpm, the temperature was 28° C., and the cultivation time was 18 hours to obtain a seed solution. The composition of the seed medium (100 mL): 10 g of sucrose, 0.3 g of yeast extract powd...

Embodiment 2

[0048] The weight percent content of each component of the liquid film is: 5% of pullulan, 5% of polyglutamic acid and the balance of water.

[0049] The preparation method of the liquid film comprises the following steps: dispersing 5% pullulan and 5% polyglutamic acid in water at 32-36° C., stirring for 5 minutes, and mixing uniformly to obtain the liquid film.

[0050] Described polyglutamic acid is prepared by following method:

[0051] Activation of bacterial classification: inoculate with the amount of 5% (v / v) on the slant of nutrient-rich solid medium, cultivate at 37 ℃ for 16 hours, prepare mature slant seeds, the composition of solid medium comprises (1L): Peptone 10g, yeast powder 5g, sodium chloride 10g, agar 20g, pH7.2;

[0052] Preparation of seed liquid: transfer one ring of the above seeds into an Erlenmeyer flask containing nutrient-rich liquid medium, cultivate at 37°C and 220rpm for 16 hours to logarithmic growth phase; prepare seed medium according to the ...

Embodiment 3

[0056] A liquid mulching film, the weight percentage of each component is: 1% of pullulan, 1% of polyglutamic acid, and the balance is water.

[0057] Described pullulan is prepared by the following method: fermentation medium composition (1L): sucrose 100g, peptone 10g, sodium chloride 5g, dipotassium hydrogen phosphate 10g, magnesium sulfate 0.6g, ferrous sulfate 1g, all the other are distilled water , initial pH6.5.

[0058] The CGMCC.NO.7055 strain was inoculated into the seed medium for shake flask activation, the rotation speed was 150 rpm, the temperature was 28°C, and the culture time was 30 hours. The activated seed culture solution was transferred to a new seed shake flask in an amount of 2% (v / v) for expanded cultivation, the rotation speed was 150 rpm, the temperature was 28° C., and the cultivation time was 20 hours. The composition of the seed medium (100 mL): 10 g of sucrose, 0.3 g of yeast extract powder, 0.25 g of sodium chloride, 0.2 g of dipotassium hydroge...

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Abstract

The invention discloses a liquid plastic film, and belongs to the technical field of agriculture. The liquid plastic film consists of the following components in percentage by weight: 1%-10% of pullulan polysaccharide, 1%-10% of polyglutamic acid and the balance of water, wherein the pullulan polysaccharide is prepared by fermenting aureobasidium pullulans of which the preservation No. is CGMCC No.7055, and the polyglutamic acid is prepared by fermenting bacillus licheniformis. The invention further discloses a preparation method and a use method of the liquid plastic film. The liquid plastic film is biodegradable and does not pollute environment, and after the plastic film is sprayed onto the surface of soil, the temperature of the soil can be raised, the evaporation of water is restrained, water content of the soil is increased, the salt content of the soil is reduced, and the soil can also be improved, so that the physicochemical property of the soil is better, and the growth of plants is promoted; besides, the liquid plastic film has the advantages of simple preparation method, low cost, good convenience in use, saving of labors and material, low cost and easiness in obtaining of raw materials, simple production equipment and technology, zero pollution in a production process, and the like.

Description

technical field [0001] The invention belongs to the field of agricultural technology. In particular, it relates to a biodegradable and non-polluting liquid mulch film and a preparation method of the mulch film. Background technique [0002] The wide application of plastic mulch has brought a technological change to my country's traditional agricultural production and laid the foundation for a substantial increase in agricultural production. However, with the progress of society, the increasing scarcity of petroleum resources, and the harm caused by plastic films to soil and the environment, It degrades in nature and soil for as long as 200-300 years, destroying the soil structure and causing "white pollution". In response to this situation, the development of liquid mulch improves soil properties, reduces labor intensity, and is easy to spray, so it is the key to solving the problem. [0003] Some of the liquid mulches currently used are made of humic acid, bio-glue or chit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L5/00C08L77/04A01G13/02C12P19/10C12P13/02C12R1/10C12R1/645
CPCY02A40/28
Inventor 乔长晟任友花李雪
Owner 天津北洋百川生物技术有限公司
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