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Functional nano-selenium and preparation thereof as well as application of functional nano-selenium in preparation of anti-bacteria and sterilization drugs

A technology of nano-selenium and functionalization, applied in antibacterial drugs, organic chemistry, pharmaceutical formulations, etc., can solve the problems of undiscovered functionalized nano-selenium antibacterial and bactericidal drugs, so as to increase drug intake and improve targeting , reduce the effect of efflux

Inactive Publication Date: 2015-03-25
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no report has been found that the functionalized nano-selenium of the present invention is used for the preparation of antibacterial and bactericidal drugs

Method used

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  • Functional nano-selenium and preparation thereof as well as application of functional nano-selenium in preparation of anti-bacteria and sterilization drugs
  • Functional nano-selenium and preparation thereof as well as application of functional nano-selenium in preparation of anti-bacteria and sterilization drugs
  • Functional nano-selenium and preparation thereof as well as application of functional nano-selenium in preparation of anti-bacteria and sterilization drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: the preparation of the nano-selenium modified by quercetin

[0050] (1) Weigh 604 mg of quercetin and dissolve it in 20 mL of methanol (analytical grade) to prepare a 0.01 mol / L storage solution, and weigh 13.5 mg of sodium selenite and dissolve it in 5 mL of distilled water to prepare a 0.01 mol / L storage solution. Dissolve 19 mg of sodium borohydride in 5 mL of distilled water to prepare a 0.01 mol / L stock solution. When in use, the above storage solution can be diluted with corresponding solvents respectively.

[0051](2) the quercetin methanol solution of 5mMol / L obtained by 10mL dilution, under constant stirring, it is added drop-wise in the sodium selenite solution of 5mMol / L obtained by 5mL dilution, mix uniformly, final quercetin and The molar ratio of sodium selenite is 2:1, add 5 mL of sodium borohydride solution with a concentration of 5 mMol / L, and stir slowly at room temperature to form a red quercetin-modified nano-selenium sol. Observed by...

Embodiment 2

[0052] Embodiment 2: Preparation of nano-selenium modified by quercetin

[0053] The 5mMol / L quercetin methanol solution obtained by 20mL dilution was added dropwise to the 5mMol / L sodium selenite solution obtained by 5mL dilution under constant stirring, and mixed evenly, finally quercetin and selenite The molar ratio of sodium is 4:1, add 5mL concentration and be the sodium borohydride solution of 5mMol / L, stir at normal temperature until the nano-selenium sol of red quercetin is formed, obtain the dispersed system that the nano-particle exists stably, its particle diameter At 80-100 nm, it is centrifuged, separated, washed and dried to obtain quercetin-modified nano-selenium (QuSeNPs).

Embodiment 3

[0054] Embodiment 3: the preparation of the nano-selenium modified by acetylcholine chloride

[0055] (1) Dissolve acetylcholine chloride in water to prepare a 0.01Mol / L stock solution.

[0056] (2) The 5mMol / L acetylcholine chloride aqueous solution obtained by 20mL dilution, under constant stirring, is added dropwise to the 5mMol / L sodium selenite solution obtained by 5mL dilution and mixed uniformly, wherein, acetylcholine chloride and The molar ratio of sodium selenite is 4:1, add a few drops of glacial acetic acid, stir vigorously in an ice bath for 10min, and mix well. Keep stirring and add 5 mL of sodium borohydride solution with a concentration of 5 mMol / L, and continue to stir slowly for 30 min until the solution turns red, that is, a red acetylcholine chloride-modified nano selenium sol is obtained. Its morphology was observed by TEANAI-10 transmission electron microscope (TEM), such as figure 2 shown. Its particle size is about 50nm. The nanometer particle can ...

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Abstract

The invention belongs to the technical field of preparation of nano-selenium, and discloses functional nano-selenium and preparation method thereof as well as application of functional nano-selenium in preparation of anti-bacteria and sterilization drugs. The functional nano-selenium is at least one of nano-selenium modified by quercetin, nano-selenium modified by acetylcholine chloride and nano-selenium modified by quercetin-acetylcholine chloride. The functional nano-selenium has the activity for killing bacteria and multidrug resistant bacteria, also has the biological activity for inhibiting the growth of bacteria and multidrug resistant bacteria, and is suitable for preparing drugs for inhibiting the growth of bacteria and multidrug resistant bacteria and sterilization drugs. The functional nano-selenium is simple in preparation method, can be obtained through in-situ reduction modification, and can be directly stored and used. The functional nano-selenium is modified through various functions, so that the targeting property of the functional nano-selenium is improved, the transportation and transmembrane absorption of the functional nano-selenium in a bacteria cell membrane are improved, the medicine intake of bacteria can be increased, and the discharge is reduced, so that the fact that drugs in bacteria cells are kept at a higher level is ensured.

Description

technical field [0001] The invention belongs to the technical field of nano-selenium preparation, and in particular relates to a functionalized nano-selenium, a preparation method thereof and an application in preparation of bacteriostatic and bactericidal drugs. Background technique [0002] The treatment of bacterial infections is one of the most challenging tasks in the biomedical field. Antibiotics have been in development for more than 70 years and have long been considered the most effective type of drug used to treat bacterial infections. However, the misuse of antibiotics will lead to more and more multi-drug resistant bacteria. [0003] Currently, multidrug-resistant superbugs are emerging at an unprecedented rate worldwide and causing public health threats, including Gram-positive vancomycin-resistant enterococci (VRE), methicillin-resistant Staphylococcus aureus (MRSA) and Gram-negative multidrug-resistant (MDR) Klebsiella pneumoniae, Acinetobacter baumannii, Ps...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07C391/00A61K31/352A61K31/221A61P31/04A61K33/04
CPCA61K33/04A61K47/545A61K47/55C07C219/06C07C391/00C07D311/30
Inventor 刘杰黄小全刘亚楠陈旭
Owner JINAN UNIVERSITY
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