Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes

A fluoroquinolone and drug resistance gene technology, applied in the detection of four tuberculosis drug resistance genes of fluoroquinolone drugs, isoniazid, rifampicin, can solve the problem of inability to determine the nature of mutation, disease deterioration, scientific, safe, Accurately guide tuberculosis patients and other issues

Inactive Publication Date: 2015-03-25
北京圣谷同创科技发展有限公司
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1. The traditional detection method of tuberculosis drug resistance-related genes can only detect a small number of specific sites at the same time, and cannot determine the mutation nature
[0004] 2. Many drug-resistant strains in actual clinical samples are drug-resistant, and there may be several strains resistant to different drugs in the strain group
Moreover, due to the uneven distribution of bacterial strains, the traditional drug-resistant site detection method can

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes
  • Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes
  • Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0053] In order to enable those skilled in the art to better understand the solution of the present invention, and to make the above-mentioned purpose, features and advantages of the present invention more obvious and understandable, the present invention will be further described in detail below in conjunction with the embodiments and the accompanying drawings of the embodiments.

[0054] The method for detecting four tuberculosis drug-resistant genes for rifampicin, isoniazid, and fluoroquinolones in Example 1 of the present invention includes the following steps:

[0055] 1. DNA quality inspection

[0056] 1) Detect DNA concentration with Qubit.

[0057] 2) For DNA with too low concentration, use agilent to detect whether the DNA is degraded by fragment size.

[0058] 3) To determine the 260 / 280 value of DNA, it is required to be greater than 1.6. In this example, the 260 / 280 value of DNA is greater than 1.6; / 280 value.

[0059] 2. Build the library

[0060] 1) Target ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes. The method comprises the following steps: designing primers capable of covering full lengths of GyrA, lnhA, katG and rpoB genes; merging the four gene primers and forming a primer pool; amplifying amplicons capable of covering full lengths of drug-resistance-related genes; establishing complete an SV-MTB library; and detecting and acquiring drug-resistant sites. By the method, multiple mutation sites of a multiple-drug-resistant strain can be detected at one time and tuberculosis patients can be scientifically, safely and accurately guided to take the medicine.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and more specifically relates to a detection method for four tuberculosis drug-resistant genes of rifampicin, isoniazid and fluoroquinolones. Background technique [0002] At present, in the diagnosis and treatment of tuberculosis, tuberculosis drug resistance test is still an important technology, but there are still some problems in the traditional detection method of tuberculosis drug resistance related genes: [0003] 1. The traditional detection method of tuberculosis resistance-related genes can only detect a small number of specific sites at the same time, and cannot determine the mutation nature. [0004] 2. Many drug-resistant strains in actual clinical samples are drug-resistant, and there may be several strains resistant to different drugs in the strain group. Moreover, due to the uneven distribution of bacterial strains, the traditional drug-resistant site detection method can det...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C40B50/06
CPCC12Q1/6806C12Q1/6869C12Q2531/113C12Q2525/191C12Q2563/159
Inventor 肖璟于丹蔡斌张庆华
Owner 北京圣谷同创科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap