Method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes

A fluoroquinolone and drug resistance gene technology, applied in the detection of four tuberculosis drug resistance genes of fluoroquinolone drugs, isoniazid, rifampicin, can solve the problem of inability to determine the nature of mutation, disease deterioration, scientific, safe, Accurately guide tuberculosis patients and other issues
CN104450877AInactive Publication Date: 2015-03-25北京圣谷同创科技发展有限公司
4 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
北京圣谷同创科技发展有限公司
Publication Date
2015-03-25
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a method for detecting four tuberculosis rifampicin, isoniazide and fluoroquinolones-resistant genes. The method comprises the following steps: designing primers capable of covering full lengths of GyrA, lnhA, katG and rpoB genes; merging the four gene primers and forming a primer pool; amplifying amplicons capable of covering full lengths of drug-resistance-related genes; establishing complete an SV-MTB library; and detecting and acquiring drug-resistant sites. By the method, multiple mutation sites of a multiple-drug-resistant strain can be detected at one time and tuberculosis patients can be scientifically, safely and accurately guided to take the medicine.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biomedical detection, and more specifically relates to a detection method for four tuberculosis drug-resistant genes of rifampicin, isoniazid and fluoroquinolones. Background technique

[0002] At present, in the diagnosis and treatment of tuberculosis, tuberculosis drug resistance test is still an important technology, but there are still some problems in the traditional detection method of tuberculosis drug resistance related genes:

[0003] 1. The traditional detection method of tuberculosis resistance-related genes can only detect a small number of specific sites at the same time, and cannot determine the mutation nature.

[0004] 2. Many drug-resistant strains in actual clinical samples are drug-resistant, and there may be several strains resistant to different drugs in the strain group. Moreover, due to the uneven distribution of bacterial strains, the traditional drug-resistant site detection method can det...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More