Digital slide scanner and scanning focus number reducing method thereof

Abstract

The invention provides a scanning focus number reducing method of a digital slide scanner. The method comprises the following steps: placing a slide on a scanning platform; scanning the slide to obtain a slide preview of the slide; adjusting the scanning area to be the entire slide; identifying foci; focusing to obtain focal lengths of foci; calculating focal planes according to the focal lengths. The mounting precision of a scanner slide mounting platform and an optical system can be effectively improved, so that a possibility is provided for clearly scanning ultra-large slides (the pixel value of a digital image can reach 100000 x 100000) with few foci. By dividing the parfocal area of the same color, the focus number is further reduced. According to the method provided by the invention, the scanning time can be entirely reduced by 20%.

Description

technical field [0001] The invention relates to a scanning method based on a digital slice scanner, in particular to a method for reducing the number of focal points based on a digital slice scanner. Background technique [0002] Digital slice scanning is to scan the traditional slices of the pathology department of the hospital through high-precision scanning to generate digital images for network transmission, intelligent analysis, remote training, etc. For digital slice scanning, the image quality of the scan is the first priority. At the same time, the scanning time is also very important. Only when the scanning time reaches a reasonable range can digital slice scanning be practical. [0003] The existing digital slice scanning process is generally divided into the following steps, [0004] 1. Slice preview, get the preview of the slice and the overview of the slice. [0005] 2. Identify the preview image and get the scanning area. [0006] 3. Identify the focus of th...

AI Technical Summary

Problems solved by technology

However, the existing manufacturing, assembly, and debugging processes cannot guarantee absolute horizontality and verticality, and the loading platform will cause a tilt of 0 microns to 50 microns; the optical axis of the optical system and the loading platform cannot be absolutely vertical, and the existing The manufacturing and installation process will cause an offset of 0° to 0.15°; the above two hardware process reasons will cause a focal length deviation of up to 100 microns between one end of the slice and the other end, which is far beyond the range of depth of field, making The focal length of the few focal points cannot represent the focal lengths of all parts of the slide, and there must be enough focal points to be able to scan all corners of the slide clearly

[0012] During the scanning process, it is necessary to ensure that the focal length of each part of the slice is within the thickness of the slice. For a standard 15mm*15mm tissue slice, under the existing optical system, there are generally 1500 to 2500 fields of view. If each field of view is focused, it takes 0.5 seconds to focus on one focus, and 750 to 1,000 seconds to focus on a standard tissue slice, which cannot meet practical requirements at all.

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