Test method for detection of tea polyphenol and flavonoid constituents of golden camellia

Abstract

The invention relates to the technical field of solid-phase extraction-gas chromatography-mass spectrum coupling, and provides a test method for detection of tea polyphenol and flavonoid constituents of golden camellia. The test method comprises solid-phase extraction adsorption-gas chromatography-mass spectrum coupling detection, wherein the solid-phase extraction is carried out by a stirring rod in a direct stirring manner; the stirring rod is coated with a graphene-polymer coating on the basis of two-dimension or three-dimension; the materials of thegraphene-polymer coating comprise graphene and a graphene modified material; thegraphene modified material comprises graphene oxide, graphene-polyethylene glycol dimethyl acrylic ester, graphene-polypyrrole, graphene-polymethyl trimethoxy silane and a graphene nanomaterial. The test method for detection of tea polyphenol and flavonoid constituents of golden camellia has the characteristics of correct detection, high sensitivity, good preferential adsorption effect and long service life.

Description

technical field [0001] The invention relates to the technical field of solid phase extraction-gas chromatography-mass spectrometry, in particular to a test method applied to the detection of tea polyphenols and flavonoids in camellia japonica. Background technique [0002] Camellia Camellia belongs to the family Theaceae and Camellia genus, and it is twin sisters with Tea, Camellia, Camellia oleifera, Camellia oleifera, and Camellia sasana. The flowers of Camellia japonica are golden yellow, dazzling, as if coated with a layer of wax, crystal clear and oily, as if translucent. Camellia chrysalis grows solitary in the leaf axils. When the flowers bloom, there are cup-shaped, pot-shaped or bowl-shaped flowers, which are delicate and colorful, beautiful and elegant. Previously, people had not seen species with golden flowers. In 1960, Chinese scientists first discovered a golden yellow camellia in Nanning, Guangxi, which was named Camellia Camellia. The oriental magic tea, w...

AI Technical Summary

Problems solved by technology

The surface of the extraction fiber stirring rod used in traditional stirring rod solid phase microextraction is coated with a thin layer of adsorption polymer materials, mostly perfluorinated resin, polypyrrole, methyl vinyl siloxane, epoxy-polydimethylsiloxane, etc. Oxane, etc. These materials are often expensive and fragile in texture. In actual processing, it is necessary to ensure the adsorption performance by thickening the coating. The thickening of the coating will not only reduce the detection sensitivity, but also improve the actual performance of the stirring rod coating. The probability of fracture during operation, when analyzing some substances such as plant active ingredients, there will also be the phenomenon of sample residue in the solid phase extraction coating material or matrix interference, which seriously affects the accuracy of detection