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Urchin yellow polysaccharide with liver protecting function and application thereof

A technology of echinacea and polysaccharides, which is applied in the field of biomedicine, can solve the problems that the hepatoprotective pharmacological effects of echinacea polysaccharides have not been recorded and reported, and achieve good hepatoprotective effect, increase survival rate, and increase the effect of IL-10

Inactive Publication Date: 2015-04-08
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hepatoprotective pharmacological effects of echinacea polysaccharides based on anti-inflammation and anti-oxidation have not been recorded or reported yet.

Method used

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  • Urchin yellow polysaccharide with liver protecting function and application thereof
  • Urchin yellow polysaccharide with liver protecting function and application thereof
  • Urchin yellow polysaccharide with liver protecting function and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: the preparation method of sea urchin yellow polysaccharide

[0048] Cut the fresh sea urchin, carefully peel off the sea urchin, and filter to remove the water in the sea urchin. Add an equal volume of acetone to the collected sea urchin yellow, stir to make it fully contact with acetone, let stand for 1h, discard the upper layer of acetone, repeat several times until the acetone layer is basically colorless, and finally evaporate the acetone under reduced pressure to obtain Sea urchin yellow acetone powder. Take 2.5kg of sea urchin yellow acetone powder and place it in a 50L glass reactor, add 25L of distilled water, extract in hot water at 90°C for an hour, repeat the extraction 3 times, combine the extracts to a total of 75L, and concentrate under reduced pressure at 50°C to 15L.

[0049] Add 90 g of papain to the concentrate, bathe in water at 60°C for 20 hours, heat at 90°C for 15 minutes to deform and inactivate the enzyme, and collect the supernata...

Embodiment 2

[0053] Embodiment 2: the mensuration of echinacea yellow polysaccharide molecular weight

[0054] Waters 600 high-performance liquid chromatography system, using Shodex KS-805 series KS-802 chromatographic column, 2414 differential refractive index detector, mobile phase is 0.1M NaNO3, sample concentration is 5.0mg / ml, injection volume is 20ul, flow rate is 1.0ml / min, the column temperature is 30°C, and the sample chromatographic curve is recorded.

[0055] The results show (attached Figure 5 ), the peak eluting time of echinacea yellow polysaccharide is 13.125min, and the purity reaches more than 99%, and the average relative molecular weight calculated according to the standard curve is 6.78×105Da.

[0056] 2. Pharmacological Experimental Research

[0057] 1. Drugs and reagents

[0058]AST, ALT, MDA, SOD, CAT, GSH-Px, GSH, NO and MPO kits were purchased from Nanjing Jiancheng Bioengineering Institute; BCA protein concentration assay kits were purchased from Beyontien Bi...

Embodiment 3

[0065] Example 3: Protective effect of echinacea polysaccharide preventive treatment on liver function in fulminant hepatitis induced by LPS / D-GalN

[0066] Methods: Male ICR mice, weighing 18-20g, were randomly divided into 12 groups according to body weight after one week of adaptive feeding, and a batch of treatment 6 hours after modeling: solvent control group (Control, 9 mice / group); LPS / D-GalN model group (Model, 9 rats / group); echinacea yellow polysaccharide low, medium and high preventive administration groups (5, 10, 20mg / kg, 9 rats / group); positive control group (silibinin, 100mg / kg , 10 / group). A batch processed 24 hours after modeling: solvent control group (Control, 9 / group); LPS / D-GalN model group (Model, 9 / group); , 20mg / kg, 9 rats / group); positive control group (silibinin, 100mg / kg, 10 rats / group). The prophylactic administration group was injected with echinacea polysaccharide through the tail vein once a day for a total of one week; the positive control gr...

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Abstract

The invention discloses an urchin yellow polysaccharide with liver protecting function, and a preparation method and application thereof. The extraction separation technique of urchin yellow polysaccharide is improved: on the basis of the existing preparation method, a DEAE Sepharose Fast Flow filler with preferable performance is adopted, ultrafiltration is carried out before the DEAE Sepharose Fast Flow ion-exchange column chromatography to perform impurity removal, and meanwhile, ultrafiltration is adopted after Sephacryl-400 gel column chromatography instead of the existing dialysis. The invention lays emphasis on application of the urchin yellow polysaccharide in preparing drugs for preventing and / or treating fulminant hepatitis. The pharmacological experimental research indicates that the urchin yellow polysaccharide disclosed by the invention can obviously enhance the survival rate of mice with fulminant hepatitis, obviously lowers the contents of the serum AST and ALT and the hepatic tissue MDA of the mice with fulminant hepatitis, enhances the liver SOD, GSH-Px and CAT activity and GSH activity, obviously lowers the level of pro-inflammatory factors TNF-alpha, IL-1beta, IL-6 and NO, enhances the level of the anti-inflammatory factor IL-10, has obvious protective action on liver injury, and is hopeful to be developed into new drugs or health-care products with liver protecting function.

Description

technical field [0001] The present invention relates to a polysaccharide, in particular to an echinacea yellow polysaccharide extracted and separated from Strongylocentrotus nudus with hepatoprotective effect and its application in the preparation of drugs or health care products for the prevention or treatment of fulminant hepatitis. It belongs to the technical field of biomedicine. Background technique [0002] Fulminant hepatitis is also called acute necrotizing hepatitis, clinically also called acute severe hepatitis or acute liver failure (acute liver failure, ALF). Immune response leads to damage and necrosis of liver cells, and its incidence accounts for 0.2-0.4% of the incidence of hepatitis. The clinical mortality rate is high, and there is still a lack of specific treatment measures. Acute hepatitis is the main cause of fulminant hepatitis is the abnormal immune response of the body, and changes in the function of the human immune system are closely related to the...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P1/16A61P37/02
Inventor 奚涛邢莹莹邱军陆圆圆周长林
Owner CHINA PHARM UNIV
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