Saponin compound with hepatoprotective effect and application of saponin compound

A compound and action technology, applied in the field of medicine, can solve the problems of inconvenient use, high price, and use restrictions, and achieve the effects of enhancing SOD activity, liver protection effect, and good effect.

Inactive Publication Date: 2014-07-16
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used liver-protecting drugs in clinical practice are limited in use due to high price, inconvenient use, or relatively large side effects.

Method used

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  • Saponin compound with hepatoprotective effect and application of saponin compound
  • Saponin compound with hepatoprotective effect and application of saponin compound
  • Saponin compound with hepatoprotective effect and application of saponin compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0025] The preparation of embodiment 1 triterpene saponin compound

[0026] Weigh 80 kg of licorice slices, add 10 times the amount of 95% ethanol to heat and reflux to extract twice, each time for 2 hours, filter, add 10 times the amount of 50% ethanol to the dregs and repeat the extraction twice, filter, combine the extracts and decompress Concentrate until there is no alcohol smell; add appropriate amount of water to the extract to form a suspension, extract the suspension with petroleum ether and ethyl acetate; prepare the extraction residue with an aqueous solution to a certain concentration, elute through D101 macroporous resin, and first use 5 column volumes were eluted with pure water with a pH of 1-2, and then 5 column volumes were eluted with 10% ethanol with a pH of 1-2, and finally eluted with 50% ethanol with a pH of 1-2, and 50% ethanol The total licorice saponin extract was obtained by dehydration and drying (yield 4.2kg).

[0027] Total saponin part by C 18 M...

Embodiment 2

[0031] Experimental Study on Anti-hepatic Injury of Triterpenoid Saponins

[0032] 1. Experimental materials and drugs

[0033] 1. Drugs and reagents

[0034] AST, ALT, MDA, SOD kits and Coomassie brilliant blue protein kits were purchased from Nanjing Jiancheng Bioengineering Institute; carbon tetrachloride (CCl 4 , analytically pure), used to prepare 0.1% peanut oil solution with peanut oil; galactosaminoside (D-GalN, Sigma company), dissolved in distilled water before use and diluted to 70mg / mL solution; bifendate tablets (Jiangsu Peng Harrier Pharmaceutical Co., Ltd.), grind it into a fine powder and add saline to make a suspension before use.

[0035] 2. Experimental animals

[0036] Clean-grade male ICR mice, weighing 18-22 g, were provided by the Experimental Animal Center of Zhejiang Province, certificate number SCXK (Zhejiang) 2008-0033.

[0037] 3. Experimental equipment

[0038] ELISA (Bio-Tek, USA); UV-2000 ultraviolet spectrophotometer (Beijing Labtech Instru...

Embodiment 3

[0069] Example 3 Cytotoxicity test

[0070] Human hepatocyte L02 cell line was cultured in DMEM medium containing 10% (volume fraction) inactivated standard fetal bovine serum, 100 U / mL penicillin, and 100 μg / mL streptomycin at 37°C, 5% CO 2 , cultured in an incubator with saturated humidity, and passaged once every 2-3 days. Cells in logarithmic growth phase were used in the experiments. Cells in the logarithmic growth phase were taken, and after trypsinization, the cell suspension was prepared with DMEM medium containing 10% fetal bovine serum, and the cell concentration was about 1×10 5 1 / ml, inoculated in a 96-well culture plate, 180 μL per well; take the saponin compound uralsaponinG prepared in Example 1 of the present invention, respectively set 2 μM, 5 μM, 10 μM, 20 μM, 50 μM, 100 μM 6 concentrations, and then add them to each well 20 μL dimethyl sulfoxide, set 4 duplicate wells for each group, set at 37°C, 5% CO 2 After culturing in the incubator for 72 hours, add ...

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Abstract

The invention discloses a new saponin compound with the hepatoprotective effect. The new triterpenoid saponin compound is obtained and separated from licorice root through spectrum and mass spectrometric data analysis after systematic study on chemical components of liquorice. Pharmacological experimental study shows that by utilizing the saponin compound, serum ALT, AST activity and liver tissue MDA content of CCl4 induced hepatic injury model mouse, ethyl alcohol induced hepatic injury model mouse and D-galactosamine induced hepatic injury model mouse can be obviously reduced, SOD activity of liver can be strengthened, and the saponin compound has obvious protective effect on liver injury. Toxicity experimental study shows that the new saponin compound with the hepatoprotective effect does not have significant toxicity and can be developed to a new medicine or health care product with the hepatoprotective effect.

Description

technical field [0001] The invention relates to a compound, in particular to a new triterpene saponin compound with hepatoprotective effect extracted from traditional Chinese medicine licorice and its application in the preparation of hepatoprotective medicine or health care product, belonging to the technical field of medicine. Background technique [0002] The liver is one of the most important organs in the human body, and liver disease is a relatively common clinical disease. Therefore, liver disease has always been one of the key research objects of medical science. Liver injury is a pathological state with outstanding characteristics shared by many liver diseases, which seriously threatens human health. Various harmful factors such as drugs, viruses, alcohol, organisms, etc. may cause liver function to be damaged to varying degrees, thereby reducing liver detoxification, excretion function, reserve and regeneration capacity, reducing liver blood flow, and aggravating ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P1/16A23L1/29A23L33/00
Inventor 陶伟伟段金廒唐于平
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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